Restriction map for pF1KB4394
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     AspEI       AspI        BamHI       BfrI        
BglII       BlpI        Bpu1102I    BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrGI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraI        
Eam1105I    Eco47III    EcoO65I     EcoRV       EcoT22I     
FseI        HindIII     HpaI        KspI        MamI        
MfeI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        ScaI        
SexAI       SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        TfiI        Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

Acc65I        911  GGTACC               G^GTACC
AccI         2161  GTCTAC               GT^[AC][GT]AC
AcsI         1951  GAATTC               [GA]^AATT[CT]
AflIII       1518  ACGCGT               A^C[GA][CT]GT
AocI         1632  CCTCAGG              CC^T[ACGT]AGG
ApoI         1951  GAATTC               [GA]^AATT[CT]
Asp718I       911  GGTACC               G^GTACC
AvrII         288  CCTAGG               C^CTAGG
BciVI        1192  GTATCC               GTATCC(6/5)
BclI         1529  TGATCA               T^GATCA
BlnI          288  CCTAGG               C^CTAGG
BspMI        2381  ACCTGC               ACCTGC(4/8)
BsrDI         174  GCAATG               GCAATG(2/0)
BstYI         695  AGATCC               [GA]^GATC[CT]
Bsu36I       1632  CCTCAGG              CC^T[ACGT]AGG
DrdI         1249  GACCTGGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1281  GAAGAG               CTCTTC(1/4)
Eco81I       1632  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1951  GAATTC               G^AATTC
FbaI         1529  TGATCA               T^GATCA
HincII       1246  GTTGAC               GT[CT]^[GA]AC
HindII       1246  GTTGAC               GT[CT]^[GA]AC
KpnI          911  GGTACC               GGTAC^C
Ksp632I      1281  GAAGAG               CTCTTC(1/4)
MflI          695  AGATCC               [GA]^GATC[CT]
MluI         1518  ACGCGT               A^CGCGT
MseI         1937  TTAA                 T^TAA
NcoI         2084  CCATGG               C^CATGG
PaeR7I       2125  CTCGAG               C^TCGAG
PflMI        2458  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI          796  GGCCAGCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          254  GCATGC               GCATG^C
StuI         2409  AGGCCT               AGG^CCT
Tru9I        1937  TTAA                 T^TAA
Tsp509I      1952  AATT                 ^AATT
Van91I       2458  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1485  CCACGTGGCCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         2125  CTCGAG               C^TCGAG
XhoII         695  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Alw44I        384  GTGCAC               G^TGCAC
             2285  GTGCAC              
AlwI          696  GATCC                GGATC(4/5)
              834  GATCC               
ApaI         1922  GGGCCC               GGGCC^C
             2139  GGGCCC              
ApaLI         384  GTGCAC               G^TGCAC
             2285  GTGCAC              
BbrPI         865  CACGTG               CAC^GTG
             1486  CACGTG              
BcgI          200  CGAAGCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1647  CGAGAACCTTGC        
BsaAI         865  CACGTG               [CT]AC^GT[GA]
             1486  CACGTG              
BsiEI         404  CGGCCG               CG[GA][CT]^CG
              788  CGGCCG              
BsmFI        1232  GGGAC                GGGAC(10/14)
             1815  GTCCC               
BsrFI         356  ACCGGC               [GA]^CCGG[CT]
             2300  ACCGGC              
BssSI         481  CACGAG               CACGAG(-5/-1)
              639  CTCGTG              
BstXI         938  CCAGTCGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1416  CCACCTGGATGG        
Cfr10I        356  ACCGGC               [GA]^CCGG[CT]
             2300  ACCGGC              
DraIII        222  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2257  CACAACGTG           
DsaI         2084  CCATGG               C^C[GA][CT]GG
             2469  CCGTGG              
EagI          404  CGGCCG               C^GGCCG
              788  CGGCCG              
EclXI         404  CGGCCG               C^GGCCG
              788  CGGCCG              
Eco52I        404  CGGCCG               C^GGCCG
              788  CGGCCG              
PmaCI         865  CACGTG               CAC^GTG
             1486  CACGTG              
PmlI          865  CACGTG               CAC^GTG
             1486  CACGTG              
SbfI         1146  CCTGCAGG             CCTGCA^GG
             2382  CCTGCAGG            
Sse8387I     1146  CCTGCAGG             CCTGCA^GG
             2382  CCTGCAGG            


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       AviII       
BalI        BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BsrI        
BssHII      BssKI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XmaCI       XmaI        
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