Restriction map for pF1KB4388
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwI        AocI        ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HapII       
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MboI        MfeI        MflI        MluI        MroI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        Sau3AI      SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SmlI        SnaBI       SphI        Sse8387I    SspBI       
SspI        SwaI        Tsp45I      Tth111I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AfaI          968  GTAC                 GT^AC
Alw44I        164  GTGCAC               G^TGCAC
AlwNI         888  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       743  AGCGCT               AGC^GCT
ApaLI         164  GTGCAC               G^TGCAC
AspHI         164  GTGCAC               G[AT]GC[AT]^C
BalI          834  TGGCCA               TGG^CCA
BbsI          478  GTCTTC               GAAGAC(2/6)
BcgI          842  GCAACCGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          773  CTAG                 C^TAG
BmyI          164  GTGCAC               G[AGT]GC[ACT]^C
BpmI          429  CTGGAG               CTGGAG(16/14)
BpuAI         478  GTCTTC               GAAGAC(2/6)
BsaBI          16  GATGGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          724  GTGCAG               GTGCAG(16/14)
BsiHKAI       164  GTGCAC               G[AT]GC[AT]^C
BsmBI         342  CGTCTC               CGTCTC(1/5)
BsmFI         250  GTCCC                GGGAC(10/14)
Bsp1286I      164  GTGCAC               G[AGT]GC[ACT]^C
BsrDI         384  CATTGC               GCAATG(2/0)
BstBI         764  TTCGAA               TT^CGAA
BstXI         306  CCAGATATTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          954  CTCAG                C^T[ACGT]AG
DraII        1023  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI          834  TGGCCA               [CT]^GGCC[GA]
EarI          336  GAAGAG               CTCTTC(1/4)
Eco47III      743  AGCGCT               AGC^GCT
EcoO109I     1023  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FauI          689  CCCGC                CCCGC(4/6)
HgaI          341  GCGTC                GACGC(5/10)
Ksp632I       336  GAAGAG               CTCTTC(1/4)
MaeI          773  CTAG                 C^TAG
MamI           16  GATGGTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         834  TGGCCA               TGG^CCA
MscI          834  TGGCCA               TGG^CCA
NdeI          215  CATATG               CA^TATG
NlaIV         249  GGTCCC               GG[ACGT]^[ACGT]CC
NspV          764  TTCGAA               TT^CGAA
PflMI         204  CCAGACTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         471  GACCCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI          968  GTAC                 GT^AC
SapI          336  GAAGAGC              GCTCTTC(1/4)
SfcI          989  CTACAG               C^T[GA][CT]AG
SfuI          764  TTCGAA               TT^CGAA
SpeI          772  ACTAGT               A^CTAGT
StuI          728  AGGCCT               AGG^CCT
TfiI          767  GAATC                G^A[AT]TC
Van91I        204  CCAGACTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          347  CCATGGCTTCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AluI          241  AGCT                 AG^CT
              826  AGCT                
AvaII         249  GGTCC                G^G[AT]CC
              756  GGTCC               
BseRI         265  CTCCTC               GAGGAG(10/8)
              289  GAGGAG              
CfoI          744  GCGC                 GCG^C
              948  GCGC                
DsaI          347  CCATGG               C^C[GA][CT]GG
              752  CCATGG              
EcoRV         515  GATATC               GAT^ATC
              670  GATATC              
HaeII         743  AGCGCT               [GA]GCGC^[CT]
              947  AGCGCC              
HhaI          744  GCGC                 GCG^C
              948  GCGC                
HinP1I        744  GCGC                 G^CGC
              948  GCGC                
MaeIII        421  GTTAC                ^GT[ACGT]AC
              884  GTAAC               
MseI          403  TTAA                 T^TAA
             1031  TTAA                
NcoI          347  CCATGG               C^CATGG
              752  CCATGG              
SfaNI         647  GCATC                GCATC(5/9)
              853  GCATC               
TaqI          469  TCGA                 T^CGA
              765  TCGA                
Tru9I         403  TTAA                 T^TAA
             1031  TTAA                
Tsp509I        76  AATT                 ^AATT
              906  AATT                
TthHB8I       469  TCGA                 T^CGA
              765  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MboII       MnlI        MslI        MvaI        MwoI        
NlaIII      PleI        Sau96I      ScrFI       StyI        
TseI        TspRI       
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