Restriction map for pF1KB4371
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwI        AlwNI       ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BbvI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KspI        MaeI        MaeII       MaeIII      MfeI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        TseI        
Tsp45I      Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I         75  GGTACC               G^GTACC
AfaI           76  GTAC                 GT^AC
AflIII        465  ACATGT               A^C[GA][CT]GT
AluI          287  AGCT                 AG^CT
AocI          409  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       305  AGCGCT               AGC^GCT
Asp718I        75  GGTACC               G^GTACC
AspHI         199  GAGCAC               G[AT]GC[AT]^C
AvaII         406  GGTCC                G^G[AT]CC
BanI           75  GGTACC               G^G[CT][GA]CC
BbsI          109  GAAGAC               GAAGAC(2/6)
BcnI          312  CCGGG                CC^[GC]GG
BpuAI         109  GAAGAC               GAAGAC(2/6)
BsaBI         207  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         301  GAGGAG               GAGGAG(10/8)
BsgI          220  GTGCAG               GTGCAG(16/14)
BsiHKAI       199  GAGCAC               G[AT]GC[AT]^C
BspLU11I      465  ACATGT               A^CATGT
BstF5I        213  CATCC                GGATG(2/0)
Bsu36I        409  CCTCAGG              CC^T[ACGT]AGG
Cac8I         172  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          306  GCGC                 GCG^C
DdeI          410  CTCAG                C^T[ACGT]AG
EarI           34  CTCTTC               CTCTTC(1/4)
Eco47III      305  AGCGCT               AGC^GCT
Eco81I        409  CCTCAGG              CC^T[ACGT]AGG
EcoNI         294  CCTGCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI        421  GCGGC                GC^[ACGT]GC
FokI          213  CATCC                GGATG(9/13)
HaeII         305  AGCGCT               [GA]GCGC^[CT]
HhaI          306  GCGC                 GCG^C
HinP1I        306  GCGC                 G^CGC
HinfI          22  GAGTC                G^A[ACGT]TC
ItaI          421  GCGGC                GC^[ACGT]GC
KpnI           75  GGTACC               GGTAC^C
Ksp632I        34  CTCTTC               CTCTTC(1/4)
MamI          207  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          213  CATCCGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          312  CCGGG                CC^[GC]GG
NlaIII        466  CATG                 CATG^
NspI          465  ACATGT               [GA]CATG^[CT]
PleI           22  GAGTC                GAGTC(4/5)
PpuMI         405  AGGTCCT              [GA]G^G[AT]CC[CT]
RsaI           76  GTAC                 GT^AC
SapI           33  GCTCTTC              GCTCTTC(1/4)
SfcI          237  CTACAG               C^T[GA][CT]AG
StuI          373  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BmyI          199  GAGCAC               G[AGT]GC[ACT]^C
              367  GTGCCC              
Bsp1286I      199  GAGCAC               G[AGT]GC[ACT]^C
              367  GTGCCC              
BsrI          120  ACTGG                ACTGG(1/-1)
              357  ACTGG               
DpnII          51  GATC                 ^GATC
              207  GATC                
DraII         324  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              405  AGGTCCT             
EcoO109I      324  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              405  AGGTCCT             
EcoT14I        71  CCAAGG               C^C[AT][AT]GG
              194  CCTTGG              
FauI          156  CCCGC                CCCGC(4/6)
              322  GCGGG               
HapII         232  CCGG                 C^CGG
              312  CCGG                
HpaII         232  CCGG                 C^CGG
              312  CCGG                
MboI           51  GATC                 ^GATC
              207  GATC                
MseI           59  TTAA                 T^TAA
              134  TTAA                
MspI          232  CCGG                 C^CGG
              312  CCGG                
NdeII          51  GATC                 ^GATC
              207  GATC                
PflMI         114  CCACACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              189  CCACCCCTTGG         
Sau3AI         51  GATC                 ^GATC
              207  GATC                
StyI           71  CCAAGG               C^C[AT][AT]GG
              194  CCTTGG              
TaqI           20  TCGA                 T^CGA
               38  TCGA                
Tru9I          59  TTAA                 T^TAA
              134  TTAA                
TspRI         119  CACTG                CA[GC]TG[ACGT][ACGT]^
              356  CACTG               
TthHB8I        20  TCGA                 T^CGA
               38  TCGA                
Van91I        114  CCACACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              189  CCACCCCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        BpmI        BsaJI       BsiYI       BslI        
BsmFI       BssKI       BstNI       Cfr13I      EcoRII      
HaeIII      HphI        MboII       MnlI        MvaI        
MwoI        NlaIV       Sau96I      ScrFI       
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