Restriction map for pF1KB4371
 (Circular) (Six-base) MAPSORT of: pF1KB4371.seq  Check: 3743  from: 1  to: 3587



pF1KB4371  3587 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    625     625 
   Size:       3587
 
Acc65I G'GTAC_C

Cuts at:    183     603     183 
   Size:        420    3167
 
AclI AA'CG_TT

Cuts at:   3427    3427 
   Size:       3587
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1412    1844    2197    2859    1412 
   Size:        432     353     662    2140

  Fragments arranged by size: 

                2140     662     432     353
 
AfeI AGC'GCT

Cuts at:    415     415 
   Size:       3587
 
AflIII A'CryG_T

Cuts at:    573     939    2739    3020     573 
   Size:        366    1800     281    1140

  Fragments arranged by size: 

                1800    1140     366     281
 
AlwNI CAG_nnn'CTG

Cuts at:    892    2330     892 
   Size:       1438    2149
 
ApaLI G'TGCA_C

Cuts at:   2425    2425 
   Size:       3587
 
ApoI r'AATT_y

Cuts at:    591    2940    3196     591 
   Size:       2349     256     982

  Fragments arranged by size: 

                2349     982     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3587
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3587
 
AvaI C'yCGr_G

Cuts at:    607     607 
   Size:       3587
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    167     200     167 
   Size:         33    3554
 
BamHI G'GATC_C

Cuts at:    612     612 
   Size:       3587
 
BanI G'GyrC_C

Cuts at:    183     603    1017    1265    1300     183 
   Size:        420     414     248      35    2470

  Fragments arranged by size: 

                2470     420     414     248      35
 
BanII G_rGCy'C

Cuts at:    601    1631     601 
   Size:       1030    2557
 
BbeI G_GCGC'C

Cuts at:   1021    1269    1021 
   Size:        248    3339
 
BbsI GAAGACnn'nnnn_

Cuts at:    224     224 
   Size:       3587
 
BciVI GTATCCnnnnn_n'

Cuts at:   1485    2541    3587    1485 
   Size:       1056    1046    1485

  Fragments arranged by size: 

                1485    1056    1046
 
BclI T'GATC_A

Cuts at:   1107    1107 
   Size:       3587
 
BglII A'GATC_T

Cuts at:   1102    3139    1102 
   Size:       2037    1550
 
BlpI GC'TnA_GC

Cuts at:    699     699 
   Size:       3587
 
Bme1580I G_kGCm'C

Cuts at:    479    1212    1305    2429     479 
   Size:        733      93    1124    1637

  Fragments arranged by size: 

                1637    1124     733      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    238     799     880    1215    3359     238 
   Size:        561      81     335    2144     466

  Fragments arranged by size: 

                2144     561     466     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1954    1954 
   Size:       3587
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    104     427     458     551     104 
   Size:        323      31      93    3140

  Fragments arranged by size: 

                3140     323      93      31
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    763    1642    2356    2654     763 
   Size:        879     714     298    1696

  Fragments arranged by size: 

                1696     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3291    3291 
   Size:       3587
 
BsaAI yAC'GTr

Cuts at:    940     940 
   Size:       3587
 
BsaBI GATnn'nnATC

Cuts at:      6     319    1101    3138       6 
   Size:        313     782    2037     455

  Fragments arranged by size: 

                2037     782     455     313
 
BsaHI Gr'CG_yC

Cuts at:   1018    1266    1968    3464    1018 
   Size:        248     702    1496    1141

  Fragments arranged by size: 

                1496    1141     702     248
 
BsaWI w'CCGG_w

Cuts at:    776    1297    1975    2386    2533    2807     776 
   Size:        521     678     411     147     274    1556

  Fragments arranged by size: 

                1556     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    424     424 
   Size:       3587
 
BseYI C'CCAG_C

Cuts at:   1012    1770    2435    2843    1012 
   Size:        758     665     408    1756

  Fragments arranged by size: 

                1756     758     665     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    349     349 
   Size:       3587
 
BsiEI CG_ry'CG

Cuts at:    104    1129    1175    2405     104 
   Size:       1025      46    1230    1286

  Fragments arranged by size: 

                1286    1230    1025      46
 
BsiHKAI G_wGCw'C

Cuts at:    311     601    1379    1569    2429     311 
   Size:        290     778     190     860    1469

  Fragments arranged by size: 

                1469     860     778     290     190
 
Bsp1286I G_dGCh'C

Cuts at:    311     479     601    1212    1305    1379    1569    1631    2429 
   Size:        168     122     611      93      74     190      62     798

Cuts at:   2429     311 
   Size:       1469

  Fragments arranged by size: 

                1469     798     611     190     168     122      93      74
                  62
 
BspHI T'CATG_A

Cuts at:   2019    2019 
   Size:       3587
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    637     851    1153    1534     637 
   Size:        214     302     381    2690

  Fragments arranged by size: 

                2690     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1879    1933    3417    3582      50 
   Size:       1829      54    1484     165      55

  Fragments arranged by size: 

                1829    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1499    1499 
   Size:       3587
 
BsrFI r'CCGG_y

Cuts at:   1585    1766    1585 
   Size:        181    3406
 
BssSI C'ACGA_G

Cuts at:   1858    2566    1858 
   Size:        708    2879
 
BstAPI GCAn_nnn'nTGC

Cuts at:    964     964 
   Size:       3587
 
BstBI TT'CG_AA

Cuts at:   1948    1948 
   Size:       3587
 
BstYI r'GATC_y

Cuts at:      1     612    1079    1102    1437    1683    1989    2001    2087 
   Size:        611     467      23     335     246     306      12      86

Cuts at:   2087    3139       1 
   Size:       1052     449

  Fragments arranged by size: 

                1052     611     467     449     335     306     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    518     518 
   Size:       3587
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1537    1718    1881    1537 
   Size:        181     163    3243

  Fragments arranged by size: 

                3243     181     163
 
BtsI GCAGTG_nn'

Cuts at:    830     830 
   Size:       3587
 
DraI TTT'AAA

Cuts at:    586     586 
   Size:       3587
 
DrdI GACnn_nn'nnGTC

Cuts at:   1293    2637    1293 
   Size:       1344    2243
 
EaeI y'GGCC_r

Cuts at:   1172    1346    1737    1764    3109    1172 
   Size:        174     391      27    1345    1650

  Fragments arranged by size: 

                1650    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1172    1172 
   Size:       3587
 
EarI CTCTTCn'nnn_

Cuts at:    148    1610    1820     148 
   Size:       1462     210    1915

  Fragments arranged by size: 

                1915    1462     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1795    2537    2683    3397    1795 
   Size:        742     146     714    1985

  Fragments arranged by size: 

                1985     742     714     146
 
EcoICRI GAG'CTC

Cuts at:    599     599 
   Size:       3587
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    104     427     458     551    1412    1844    2197    2859     104 
   Size:        323      31      93     861     432     353     662     832

  Fragments arranged by size: 

                 861     832     662     432     353     323      93      31
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    406     406 
   Size:       3587
 
EcoO109I rG'GnC_Cy

Cuts at:    433     514     726     433 
   Size:         81     212    3294

  Fragments arranged by size: 

                3294     212      81
 
EcoRI G'AATT_C

Cuts at:    591     591 
   Size:       3587
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1606    1606 
   Size:       3587
 
FspI TGC'GCA

Cuts at:   1368    1368 
   Size:       3587
 
HaeII r_GCGC'y

Cuts at:    417    1021    1269    2499    3270     417 
   Size:        604     248    1230     771     734

  Fragments arranged by size: 

                1230     771     734     604     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1429    1459    1461    1491    1429 
   Size:         30       2      30    3525

  Fragments arranged by size: 

                3525      30      30       2
 
HincII GTy'rAC

Cuts at:    626     626 
   Size:       3587
 
KasI G'GCGC_C

Cuts at:   1017    1265    1017 
   Size:        248    3339
 
KpnI G_GTAC'C

Cuts at:    187     607     187 
   Size:        420    3167
 
MluI A'CGCG_T

Cuts at:   3020    3020 
   Size:       3587
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2346    2530    2346 
   Size:        184    3403
 
MscI TGG'CCA

Cuts at:   1348    1348 
   Size:       3587
 
MslI CAynn'nnrTG

Cuts at:    325    1576    1703     325 
   Size:       1251     127    2209

  Fragments arranged by size: 

                2209    1251     127
 
MspA1I CmG'CkG

Cuts at:    698    1012    1372    2156    2401     698 
   Size:        314     360     784     245    1884

  Fragments arranged by size: 

                1884     784     360     314     245
 
NaeI GCC'GGC

Cuts at:   1768    1768 
   Size:       3587
 
NarI GG'CG_CC

Cuts at:   1018    1266    1018 
   Size:        248    3339
 
NgoMIV G'CCGG_C

Cuts at:   1766    1766 
   Size:       3587
 
NspI r_CATG'y

Cuts at:    577     640    1140    2743     577 
   Size:         63     500    1603    1421

  Fragments arranged by size: 

                1603    1421     500      63
 
PciI A'CATG_T

Cuts at:    573    2739     573 
   Size:       2166    1421
 
PflMI CCAn_nnn'nTGG

Cuts at:    228     303     228 
   Size:         75    3512
 
PfoI T'CCnGG_A

Cuts at:    567     567 
   Size:       3587
 
PmeI GTTT'AAAC

Cuts at:    586     586 
   Size:       3587
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2033    2065    2033 
   Size:         32    3555
 
PpuMI rG'GwC_Cy

Cuts at:    514     514 
   Size:       3587
 
PstI C_TGCA'G

Cuts at:    634    1319     634 
   Size:        685    2902
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3587
 
PvuII CAG'CTG

Cuts at:   1012    1372    1012 
   Size:        360    3227
 
RsrII CG'GwC_CG

Cuts at:   1782    1782 
   Size:       3587
 
SacI G_AGCT'C

Cuts at:    601     601 
   Size:       3587
 
SalI G'TCGA_C

Cuts at:    624     624 
   Size:       3587
 
SapI GCTCTTCn'nnn_

Cuts at:    148    1610    1820     148 
   Size:       1462     210    1915

  Fragments arranged by size: 

                1915    1462     210
 
SbfI CC_TGCA'GG

Cuts at:    634     634 
   Size:       3587
 
ScaI AGT'ACT

Cuts at:   3104    3104 
   Size:       3587
 
SfcI C'TryA_G

Cuts at:     33     345     630    1315    2283    2474      33 
   Size:        312     285     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     312     285     191
 
SfoI GGC'GCC

Cuts at:   1019    1267    1019 
   Size:        248    3339
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3587
 
SmaI CCC'GGG

Cuts at:    609     609 
   Size:       3587
 
SmlI C'TyrA_G

Cuts at:    742    1657    2371    2633     742 
   Size:        915     714     262    1696

  Fragments arranged by size: 

                1696     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3587
 
SphI G_CATG'C

Cuts at:    640    1140     640 
   Size:        500    3087
 
StuI AGG'CCT

Cuts at:    483     483 
   Size:       3587
 
StyI C'CwwG_G

Cuts at:    179     302     721    1075     179 
   Size:        123     419     354    2691

  Fragments arranged by size: 

                2691     419     354     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1170    1303    1762    1971    1170 
   Size:        133     459     209    2786

  Fragments arranged by size: 

                2786     459     209     133
 
TatI w'GTAC_w

Cuts at:   3102    3102 
   Size:       3587
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3104    3136    3104 
   Size:         32    3555
 
Tth111I GACn'n_nGTC

Cuts at:   1384    1384 
   Size:       3587
 
XbaI T'CTAG_A

Cuts at:    618     618 
   Size:       3587
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1082    1082 
   Size:       3587
 
XmaI C'CCGG_G

Cuts at:    607     607 
   Size:       3587
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AgeI     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BsiWI     BsmI    BsmBI    BspEI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I     BtgI     ClaI    CspCI   DraIII
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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