Restriction map for pF1KB4351
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbrPI       
BcgI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmFI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FseI        FspI        HgaI        HindIII     
HpaI        HphI        KpnI        MfeI        MluI        
MroI        MslI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       PstI        PvuI        RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TspRI       
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AclI          430  AACGTT               AA^CGTT
AcyI          100  GGCGCC               G[GA]^CG[CT]C
AflIII        568  ACACGT               A^C[GA][CT]GT
AlwNI         916  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           27  GGGCCC               GGGCC^C
AvaI          954  CCCGAG               C^[CT]CG[GA]G
BalI          563  TGGCCA               TGG^CCA
BamHI         733  GGATCC               G^GATCC
BbeI          100  GGCGCC               GGCGC^C
BbvCI         360  CCTCAGC              CCTCAGC(-5/-2)
BciVI        1069  GGATAC               GTATCC(6/5)
BclI          534  TGATCA               T^GATCA
BcnI           30  CCCGG                CC^[GC]GG
BsaHI         100  GGCGCC               G[GA]^CG[CT]C
BsgI          436  CTGCAC               GTGCAG(16/14)
BsmAI        1048  GAGAC                GTCTC(1/5)
BsmI          647  GCATTC               GAATGC(1/-1)
BsoBI         954  CCCGAG               C^[CT]CG[GA]G
BsrDI         365  GCAATG               GCAATG(2/0)
BstAPI        110  GCATGGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1005  GGATG                GGATG(2/0)
BstXI         851  CCAAAGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI          771  TTTAAA               TTT^AAA
DraII         314  AGGACCT              [GA]G^G[ACGT]CC[CT]
EaeI          563  TGGCCA               [CT]^GGCC[GA]
EcoO109I      314  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         600  GAATTC               G^AATTC
FbaI          534  TGATCA               T^GATCA
FokI         1005  GGATG                GGATG(9/13)
HaeII         100  GGCGCC               [GA]GCGC^[CT]
HapII          31  CCGG                 C^CGG
Hin1I         100  GGCGCC               G[GA]^CG[CT]C
HpaII          31  CCGG                 C^CGG
KasI          100  GGCGCC               G^GCGCC
KspI           42  CCGCGG               CCGC^GG
MluNI         563  TGGCCA               TGG^CCA
MscI          563  TGGCCA               TGG^CCA
MspI           31  CCGG                 C^CGG
NarI          100  GGCGCC               GG^CGCC
NciI           30  CCCGG                CC^[GC]GG
NcoI          820  CCATGG               C^CATGG
PleI          847  GACTC                GAGTC(4/5)
PpuMI         314  AGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I      430  AACGTT               AA^CGTT
PvuII         706  CAGCTG               CAG^CTG
SacII          42  CCGCGG               CCGC^GG
SfcI          638  CTGTAG               C^T[GA][CT]AG
SfoI          100  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI          600  GAATTC               [GA]^AATT[CT]
              768  AAATTT              
AocI          204  CCTGAGG              CC^T[ACGT]AGG
             1015  CCTAAGG             
ApoI          600  GAATTC               [GA]^AATT[CT]
              768  AAATTT              
AvaII          39  GGTCC                G^G[AT]CC
              315  GGACC               
BanI          100  GGCGCC               G^G[CT][GA]CC
              816  GGTGCC              
BbsI          348  GAAGAC               GAAGAC(2/6)
              748  GAAGAC              
BbvI          465  GCTGC                GCAGC(8/12)
              644  GCTGC               
BfaI          779  CTAG                 C^TAG
             1055  CTAG                
BpmI          584  CTCCAG               CTGGAG(16/14)
              937  CTGGAG              
BpuAI         348  GAAGAC               GAAGAC(2/6)
              748  GAAGAC              
BspMI         461  ACCTGC               ACCTGC(4/8)
               48  GCAGGT              
Bsu36I        204  CCTGAGG              CC^T[ACGT]AGG
             1015  CCTAAGG             
DsaI           42  CCGCGG               C^C[GA][CT]GG
              820  CCATGG              
EarI          797  CTCTTC               CTCTTC(1/4)
             1045  GAAGAG              
Eco81I        204  CCTGAGG              CC^T[ACGT]AGG
             1015  CCTAAGG             
EcoT14I       605  CCAAGG               C^C[AT][AT]GG
              820  CCATGG              
HincII        136  GTCAAC               GT[CT]^[GA]AC
              879  GTTGAC              
HindII        136  GTCAAC               GT[CT]^[GA]AC
              879  GTTGAC              
Ksp632I       797  CTCTTC               CTCTTC(1/4)
             1045  GAAGAG              
MaeI          779  CTAG                 C^TAG
             1055  CTAG                
MseI          409  TTAA                 T^TAA
              772  TTAA                
MspA1I         42  CCGCGG               C[AC]G^C[GT]G
              706  CAGCTG              
PflMI         566  CCACACGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              714  CCACATTTTGG         
SmlI          448  CTCAAG               C^T[CT][GA]AG
              487  CTCAAG              
StyI          605  CCAAGG               C^C[AT][AT]GG
              820  CCATGG              
TfiI          254  GAATC                G^A[AT]TC
             1058  GATTC               
Tru9I         409  TTAA                 T^TAA
              772  TTAA                
TseI          465  GCTGC                G^C[AT]GC
              644  GCTGC               
Van91I        566  CCACACGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              714  CCACATTTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       BanII       BglII       BmyI        BsaBI       
BsaJI       BsiHKAI     BsiYI       BslI        Bsp1286I    
BssKI       BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EcoRII      
FauI        Fnu4HI      HaeIII      HhaI        HinP1I      
HinfI       ItaI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MnlI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        Sau3AI      Sau96I      ScrFI       TaqI        
Tsp45I      Tsp509I     TthHB8I     XhoII       
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