Restriction map for pF1KB4329
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflII       
AflIII      AgeI        Alw44I      ApaLI       AscI        
Asp700I     Asp718I     AspI        AviII       BbrPI       
BbvCI       BcgI        BclI        BfrI        BglII       
BlpI        Bpu1102I    BsiEI       BsiWI       Bsp1407I    
BspLU11I    BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstEII      BstPI       BstUI       BstZ17I     
CelII       Cfr10I      CpoI        DraII       EagI        
EarI        EclXI       Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoT22I     FbaI        FseI        FspI        
HgaI        HpaI        KpnI        Ksp632I     KspI        
MluI        MvnI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SgrAI       SmaI        SpeI        SphI        Sse8387I    
SspBI       Tth111I     XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AccIII       2176  TCCGGA               T^CCGGA
AcsI         1760  GAATTC               [GA]^AATT[CT]
AcyI         1580  GGCGCC               G[GA]^CG[CT]C
AhdI          670  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1057  CAGGTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1166  AGCGCT               AGC^GCT
ApaI         2373  GGGCCC               GGGCC^C
ApoI         1760  GAATTC               [GA]^AATT[CT]
AseI          610  ATTAAT               AT^TAAT
AsnI          610  ATTAAT               AT^TAAT
AspEI         670  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1331  CTCGAG               C^[CT]CG[GA]G
AvrII        1965  CCTAGG               C^CTAGG
BamHI         399  GGATCC               G^GATCC
BbeI         1580  GGCGCC               GGCGC^C
BciVI        2711  GTATCC               GTATCC(6/5)
BlnI         1965  CCTAGG               C^CTAGG
BsaAI        2708  TACGTA               [CT]AC^GT[GA]
BsaHI        1580  GGCGCC               G[GA]^CG[CT]C
BsaI         1559  GAGACC               GGTCTC(1/5)
BsaWI        2176  TCCGGA               [AT]^CCGG[AT]
BseAI        2176  TCCGGA               T^CCGGA
BsmBI        1651  GAGACG               CGTCTC(1/5)
BsmI          637  GAATGC               GAATGC(1/-1)
BsoBI        1331  CTCGAG               C^[CT]CG[GA]G
BspDI        2431  ATCGAT               AT^CGAT
BspEI        2176  TCCGGA               T^CCGGA
BstBI        1109  TTCGAA               TT^CGAA
Cac8I        1122  GCGGGC               GC[ACGT]^[ACGT]GC
ClaI         2431  ATCGAT               AT^CGAT
DrdI         1976  GACTAGATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      670  GACTGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1166  AGCGCT               AGC^GCT
EcoRI        1760  GAATTC               G^AATTC
EcoRV         448  GATATC               GAT^ATC
Hin1I        1580  GGCGCC               G[GA]^CG[CT]C
HincII       1099  GTTGAC               GT[CT]^[GA]AC
HindII       1099  GTTGAC               GT[CT]^[GA]AC
KasI         1580  GGCGCC               G^GCGCC
MfeI         1229  CAATTG               C^AATTG
MroI         2176  TCCGGA               T^CCGGA
MunI         1229  CAATTG               C^AATTG
NarI         1580  GGCGCC               GG^CGCC
NcoI          326  CCATGG               C^CATGG
NspI         2654  ACATGC               [GA]CATG^[CT]
NspV         1109  TTCGAA               TT^CGAA
PaeR7I       1331  CTCGAG               C^TCGAG
PflMI         459  CCAAATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1903  GACCTCGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         610  ATTAAT               AT^TAAT
PvuII        2448  CAGCTG               CAG^CTG
SacI         2756  GAGCTC               GAGCT^C
SexAI        2109  ACCTGGT              A^CC[AT]GGT
SfoI         1580  GGCGCC               GGC^GCC
SfuI         1109  TTCGAA               TT^CGAA
SnaBI        2708  TACGTA               TAC^GTA
SspI           42  AATATT               AAT^ATT
StuI          937  AGGCCT               AGG^CCT
SwaI         2573  ATTTAAAT             ATTT^AAAT
Van91I        459  CCAAATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          798  TCTAGA               T^CTAGA
XhoI         1331  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          343  GTAGAC               GT^[AC][GT]AC
             2788  GTCTAC              
AfaI          981  GTAC                 GT^AC
             2661  GTAC                
AspHI        1794  GAGCAC               G[AT]GC[AT]^C
             2756  GAGCTC              
BalI          248  TGGCCA               TGG^CCA
             2309  TGGCCA              
BanI         1580  GGCGCC               G^G[CT][GA]CC
             2106  GGCACC              
BanII        2373  GGGCCC               G[GA]GC[CT]^C
             2756  GAGCTC              
BpmI         2407  CTCCAG               CTGGAG(16/14)
             1408  CTGGAG              
BsgI          892  GTGCAG               GTGCAG(16/14)
             1438  GTGCAG              
BsiHKAI      1794  GAGCAC               G[AT]GC[AT]^C
             2756  GAGCTC              
BspHI        1022  TCATGA               T^CATGA
             2381  TCATGA              
BsrBI         373  CCGCTC               CCGCTC(-3/-3)
              724  GAGCGG              
BsrDI        1050  CATTGC               GCAATG(2/0)
             1181  CATTGC              
BstAPI       2023  GCAACCTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2649  GCAGGACATGC         
BstXI        1142  CCATCAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1940  CCATGCAGATGG        
DraI         1893  TTTAAA               TTT^AAA
             2574  TTTAAA              
DraIII       1290  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2108  CACCTGGTG           
DsaI          125  CCGTGG               C^C[GA][CT]GG
              326  CCATGG              
FauI          726  GCGGG                CCCGC(4/6)
             1122  GCGGG               
HindIII       199  AAGCTT               A^AGCTT
             1401  AAGCTT              
MaeII         158  ACGT                 A^CGT
             2709  ACGT                
MluNI         248  TGGCCA               TGG^CCA
             2309  TGGCCA              
MscI          248  TGGCCA               TGG^CCA
             2309  TGGCCA              
MspA1I        773  CAGCGG               C[AC]G^C[GT]G
             2448  CAGCTG              
RcaI         1022  TCATGA               T^CATGA
             2381  TCATGA              
RsaI          981  GTAC                 GT^AC
             2661  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AluI        AlwI        AocI        AvaII       
BbsI        BbvI        BcnI        BfaI        BglI        
BmyI        BpuAI       BsaBI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      CfoI        Cfr13I      DdeI        
DpnII       EaeI        Eco81I      EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MamI        MboI        
MboII       MflI        MnlI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       
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