Restriction map for
pF1KB4325
(Circular) (Six-base) MAPSORT of: pF1KB4325.seq Check: 8474 from: 1 to: 4421
pF1KB4325 4421 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 718 718
Size: 4421
AccI GT'mk_AC
Cuts at: 380 1459 380
Size: 1079 3342
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 814 1045 1240 2246 2678 3031 3693 371
Size: 443 231 195 1006 432 353 662 1099
Fragments arranged by size:
1099 1006 662 443 432 353 231 195
AflIII A'CryG_T
Cuts at: 1773 3573 3854 1773
Size: 1800 281 2340
Fragments arranged by size:
2340 1800 281
AleI CACnn'nnGTG
Cuts at: 922 1231 922
Size: 309 4112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1390 1422 1390
Size: 32 4389
AlwNI CAG_nnn'CTG
Cuts at: 448 1263 1726 3164 448
Size: 815 463 1438 1705
Fragments arranged by size:
1705 1438 815 463
ApaLI G'TGCA_C
Cuts at: 3259 3259
Size: 4421
ApoI r'AATT_y
Cuts at: 390 1005 1055 1425 3774 4030 390
Size: 615 50 370 2349 256 781
Fragments arranged by size:
2349 781 615 370 256 50
AseI AT'TA_AT
Cuts at: 20 20
Size: 4421
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 1441 1441
Size: 4421
BamHI G'GATC_C
Cuts at: 812 1446 812
Size: 634 3787
BanI G'GyrC_C
Cuts at: 652 678 1357 1437 1851 2099 2134 652
Size: 26 679 80 414 248 35 2939
Fragments arranged by size:
2939 679 414 248 80 35 26
BanII G_rGCy'C
Cuts at: 232 349 1193 1435 2465 232
Size: 117 844 242 1030 2188
Fragments arranged by size:
2188 1030 844 242 117
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BbsI GAAGACnn'nnnn_
Cuts at: 1056 1056
Size: 4421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 909 943 909
Size: 34 4387
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3375 4421 2319
Size: 1056 1046 2319
Fragments arranged by size:
2319 1056 1046
BclI T'GATC_A
Cuts at: 1941 1941
Size: 4421
BglI GCCn_nnn'nGGC
Cuts at: 112 1285 112
Size: 1173 3248
BglII A'GATC_T
Cuts at: 849 1936 3973 849
Size: 1087 2037 1297
Fragments arranged by size:
2037 1297 1087
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 1362 2046 2139 3263 1362
Size: 684 93 1124 2520
Fragments arranged by size:
2520 1124 684 93
BmrI ACTGGGnnnn_n'
Cuts at: 1633 1714 2049 4193 1633
Size: 81 335 2144 1861
Fragments arranged by size:
2144 1861 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 4421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 1597 2476 3190 3488 517
Size: 1080 879 714 298 1450
Fragments arranged by size:
1450 1080 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1084 4125 1084
Size: 3041 1380
BsaAI yAC'GTr
Cuts at: 1774 1774
Size: 4421
BsaBI GATnn'nnATC
Cuts at: 6 1935 3972 6
Size: 1929 2037 455
Fragments arranged by size:
2037 1929 455
BsaHI Gr'CG_yC
Cuts at: 685 899 1852 2100 2802 4298 685
Size: 214 953 248 702 1496 808
Fragments arranged by size:
1496 953 808 702 248 214
BsaWI w'CCGG_w
Cuts at: 1610 2131 2809 3220 3367 3641 1610
Size: 521 678 411 147 274 2390
Fragments arranged by size:
2390 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1199 1229 1390 1420 1199
Size: 30 161 30 4200
Fragments arranged by size:
4200 161 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1360 1360
Size: 4421
BseYI C'CCAG_C
Cuts at: 626 824 1846 2604 3269 3677 626
Size: 198 1022 758 665 408 1370
Fragments arranged by size:
1370 1022 758 665 408 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 640 1312 640
Size: 672 3749
BsiEI CG_ry'CG
Cuts at: 104 758 1963 2009 3239 104
Size: 654 1205 46 1230 1286
Fragments arranged by size:
1286 1230 1205 654 46
BsiHKAI G_wGCw'C
Cuts at: 1193 1435 2213 2403 3263 1193
Size: 242 778 190 860 2351
Fragments arranged by size:
2351 860 778 242 190
BsmI GAATG_Cn'
Cuts at: 485 485
Size: 4421
Bsp1286I G_dGCh'C
Cuts at: 232 349 1193 1362 1435 2046 2139 2213 2403
Size: 117 844 169 73 611 93 74 190
Cuts at: 2403 2465 3263 232
Size: 62 798 1390
Fragments arranged by size:
1390 844 798 611 190 169 117 93
74 73 62
BspHI T'CATG_A
Cuts at: 1301 2853 1301
Size: 1552 2869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 718 1287 1404 1471 1685 1987 2368 718
Size: 569 117 67 214 302 381 2771
Fragments arranged by size:
2771 569 381 302 214 117 67
BsrBI CCG'CTC
Cuts at: 50 2713 2767 4251 4416 50
Size: 2663 54 1484 165 55
Fragments arranged by size:
2663 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 545 2333 545
Size: 1788 2633
BsrFI r'CCGG_y
Cuts at: 681 1124 2419 2600 681
Size: 443 1295 181 2502
Fragments arranged by size:
2502 1295 443 181
BssSI C'ACGA_G
Cuts at: 2692 3400 2692
Size: 708 3713
BstAPI GCAn_nnn'nTGC
Cuts at: 159 1798 159
Size: 1639 2782
BstBI TT'CG_AA
Cuts at: 2782 2782
Size: 4421
BstYI r'GATC_y
Cuts at: 1 192 438 812 849 1446 1913 1936 2271
Size: 191 246 374 37 597 467 23 335
Cuts at: 2271 2517 2823 2835 2921 3973 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1052 597 467 449 374 335 306 246
246 191 86 37 23 12
Bsu36I CC'TnA_GG
Cuts at: 665 665
Size: 4421
BtgI C'CryG_G
Cuts at: 713 713
Size: 4421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2371 2552 2715 2371
Size: 181 163 4077
Fragments arranged by size:
4077 181 163
BtsI GCAGTG_nn'
Cuts at: 1664 1664
Size: 4421
DraI TTT'AAA
Cuts at: 1420 1420
Size: 4421
DrdI GACnn_nn'nnGTC
Cuts at: 2127 3471 2127
Size: 1344 3077
EaeI y'GGCC_r
Cuts at: 1126 2006 2180 2571 2598 3943 1126
Size: 880 174 391 27 1345 1604
Fragments arranged by size:
1604 1345 880 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 946 2444 2654 946
Size: 1498 210 2713
Fragments arranged by size:
2713 1498 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2629 3371 3517 4231 2629
Size: 742 146 714 2819
Fragments arranged by size:
2819 742 714 146
EcoICRI GAG'CTC
Cuts at: 1191 1433 1191
Size: 242 4179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 371 814 1045 1240 2246 2678 3031 3693 371
Size: 443 231 195 1006 432 353 662 1099
Fragments arranged by size:
1099 1006 662 443 432 353 231 195
EcoO109I rG'GnC_Cy
Cuts at: 1560 1560
Size: 4421
EcoRI G'AATT_C
Cuts at: 1055 1425 1055
Size: 370 4051
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4421
FspI TGC'GCA
Cuts at: 484 2202 484
Size: 1718 2703
HaeII r_GCGC'y
Cuts at: 1855 2103 3333 4104 1855
Size: 248 1230 771 2172
Fragments arranged by size:
2172 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 865 897 981 1013 1199 1231 1312 1344 2263
Size: 32 84 32 186 32 81 32 919
Cuts at: 2263 2293 2295 2325 865
Size: 30 2 30 2961
Fragments arranged by size:
2961 919 186 84 81 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 513 1026 1460 513
Size: 513 434 3474
Fragments arranged by size:
3474 513 434
HpaI GTT'AAC
Cuts at: 513 513
Size: 4421
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1181 3180 3364 1052
Size: 129 1999 184 2109
Fragments arranged by size:
2109 1999 184 129
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 869 922 1231 1243 2410 2537 869
Size: 53 309 12 1167 127 2753
Fragments arranged by size:
2753 1167 309 127 53 12
MspA1I CmG'CkG
Cuts at: 1382 1532 1846 2206 2990 3235 1382
Size: 150 314 360 784 245 2568
Fragments arranged by size:
2568 784 360 314 245 150
NaeI GCC'GGC
Cuts at: 683 2602 683
Size: 1919 2502
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NcoI C'CATG_G
Cuts at: 713 713
Size: 4421
NgoMIV G'CCGG_C
Cuts at: 681 2600 681
Size: 1919 2502
NspI r_CATG'y
Cuts at: 916 1474 1974 3577 916
Size: 558 500 1603 1760
Fragments arranged by size:
1760 1603 558 500
PciI A'CATG_T
Cuts at: 3573 3573
Size: 4421
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1390 1422 2867 2899 1390
Size: 32 1445 32 2912
Fragments arranged by size:
2912 1445 32 32
PstI C_TGCA'G
Cuts at: 538 698 1415 1468 2153 538
Size: 160 717 53 685 2806
Fragments arranged by size:
2806 717 685 160 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 1846 2206 1846
Size: 360 4061
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 1193 1435 1193
Size: 242 4179
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 946 2444 2654 946
Size: 1498 210 2713
Fragments arranged by size:
2713 1498 210
SbfI CC_TGCA'GG
Cuts at: 1415 1468 1415
Size: 53 4368
ScaI AGT'ACT
Cuts at: 3938 3938
Size: 4421
SfcI C'TryA_G
Cuts at: 33 534 694 1411 1464 2149 3117 3308 33
Size: 501 160 717 53 685 968 191 1146
Fragments arranged by size:
1146 968 717 685 501 191 160 53
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 1443 1443
Size: 4421
SmlI C'TyrA_G
Cuts at: 496 1576 2491 3205 3467 496
Size: 1080 915 714 262 1450
Fragments arranged by size:
1450 1080 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 916 1474 1974 916
Size: 558 500 3363
Fragments arranged by size:
3363 558 500
StyI C'CwwG_G
Cuts at: 713 768 1555 1909 713
Size: 55 787 354 3225
Fragments arranged by size:
3225 787 354 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2004 2137 2596 2805 2004
Size: 133 459 209 3620
Fragments arranged by size:
3620 459 209 133
TatI w'GTAC_w
Cuts at: 3936 3936
Size: 4421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 662 694 3938 3970 662
Size: 32 3244 32 1113
Fragments arranged by size:
3244 1113 32 32
Tth111I GACn'n_nGTC
Cuts at: 792 2218 792
Size: 1426 2995
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 720 1916 720
Size: 1196 3225
XmaI C'CCGG_G
Cuts at: 1441 1441
Size: 4421
XmnI GAAnn'nnTTC
Cuts at: 424 424
Size: 4421
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI