Restriction map for pF1KB4237
 (Linear) (Six-base) MAPSORT of: KIBB4237.seq  Check: 1565  from: 1  to: 2451



KIBB4237.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     345     427    2451 
   Size:        345      82    2024

  Fragments arranged by size: 

                2024     345      82
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     628     913    1421    2451 
   Size:        411     217     285     508    1030

  Fragments arranged by size: 

                1030     508     411     285     217
 
AflIII A'CryG_T

Cuts at:      0     569     956    2451 
   Size:        569     387    1495

  Fragments arranged by size: 

                1495     569     387
 
AleI CACnn'nnGTG

Cuts at:      0    1381    2451 
   Size:       1381    1070
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2053    2085    2451 
   Size:       2053      32     366

  Fragments arranged by size: 

                2053     366      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     622    2451 
   Size:        622    1829
 
ApaI G_GGCC'C

Cuts at:      0     698    1524    1903    2451 
   Size:        698     826     379     548

  Fragments arranged by size: 

                 826     698     548     379
 
ApaLI G'TGCA_C

Cuts at:      0     324    2451 
   Size:        324    2127
 
ApoI r'AATT_y

Cuts at:      0    1868    1939    1951    2326    2451 
   Size:       1868      71      12     375     125

  Fragments arranged by size: 

                1868     375     125      71      12
 
AseI AT'TA_AT

Cuts at:      0    1607    2451 
   Size:       1607     844
 
AvaI C'yCGr_G

Cuts at:      0      73     270     661    1039    1544    1779    2033    2451 
   Size:         73     197     391     378     505     235     254     418

  Fragments arranged by size: 

                 505     418     391     378     254     235     197      73
            
 
BamHI G'GATC_C

Cuts at:      0    1957    2451 
   Size:       1957     494
 
BanI G'GyrC_C

Cuts at:      0      68     136     252    2451 
   Size:         68      68     116    2199

  Fragments arranged by size: 

                2199     116      68      68
 
BanII G_rGCy'C

Cuts at:      0     698    1471    1524    1574    1719    1903    2451 
   Size:        698     773      53      50     145     184     548

  Fragments arranged by size: 

                 773     698     548     184     145      53      50
 
BbeI G_GCGC'C

Cuts at:      0      72     140    2451 
   Size:         72      68    2311

  Fragments arranged by size: 

                2311      72      68
 
BbvCI CC'TCA_GC

Cuts at:      0    1127    2451 
   Size:       1127    1324
 
BclI T'GATC_A

Cuts at:      0    1889    2451 
   Size:       1889     562
 
BglII A'GATC_T

Cuts at:      0    2294    2451 
   Size:       2294     157
 
BlpI GC'TnA_GC

Cuts at:      0    1573    2451 
   Size:       1573     878
 
Bme1580I G_kGCm'C

Cuts at:      0      31     328     698    1524    1903    2420    2451 
   Size:         31     297     370     826     379     517      31

  Fragments arranged by size: 

                 826     517     379     370     297      31      31
 
BmgBI CAC'GTC

Cuts at:      0     570    1990    2451 
   Size:        570    1420     461

  Fragments arranged by size: 

                1420     570     461
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1383    1679    2451 
   Size:       1383     296     772

  Fragments arranged by size: 

                1383     772     296
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2338    2451 
   Size:       2338     113
 
Bpu10I CC'TnA_GC

Cuts at:      0     260    1127    2451 
   Size:        260     867    1324

  Fragments arranged by size: 

                1324     867     260
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421    2451 
   Size:        421    2030
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     658    1280    2451 
   Size:        658     622    1171

  Fragments arranged by size: 

                1171     658     622
 
BsaBI GATnn'nnATC

Cuts at:      0    1535    2451 
   Size:       1535     916
 
BsaHI Gr'CG_yC

Cuts at:      0      69     137     810    2451 
   Size:         69      68     673    1641

  Fragments arranged by size: 

                1641     673      69      68
 
BsaWI w'CCGG_w

Cuts at:      0    1289    2451 
   Size:       1289    1162
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1159    1189    1918    1948    2451 
   Size:       1159      30     729      30     503

  Fragments arranged by size: 

                1159     729     503      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1064    1067    1144    1220    2451 
   Size:       1064       3      77      76    1231

  Fragments arranged by size: 

                1231    1064      77      76       3
 
BseYI C'CCAG_C

Cuts at:      0     307     528     721    1254    2451 
   Size:        307     221     193     533    1197

  Fragments arranged by size: 

                1197     533     307     221     193
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1150    2451 
   Size:       1150    1301
 
BsiEI CG_ry'CG

Cuts at:      0     237    2451 
   Size:        237    2214
 
BsiHKAI G_wGCw'C

Cuts at:      0     283     328    1471    1719    2451 
   Size:        283      45    1143     248     732

  Fragments arranged by size: 

                1143     732     283     248      45
 
BsmI GAATG_Cn'

Cuts at:      0    1329    2451 
   Size:       1329    1122
 
Bsp1286I G_dGCh'C

Cuts at:      0      31     283     328     698    1471    1524    1574    1719 
   Size:         31     252      45     370     773      53      50     145

Cuts at:   1719    1903    2420    2451 
   Size:        184     517      31

  Fragments arranged by size: 

                 773     517     370     252     184     145      53      50
                  45      31      31
 
BspHI T'CATG_A

Cuts at:      0    1332    2451 
   Size:       1332    1119
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     345     427     692    2451 
   Size:        345      82     265    1759

  Fragments arranged by size: 

                1759     345     265      82
 
BsrDI GCAATG_nn'

Cuts at:      0     716    2181    2213    2451 
   Size:        716    1465      32     238

  Fragments arranged by size: 

                1465     716     238      32
 
BsrFI r'CCGG_y

Cuts at:      0     232     249     827    1363    2451 
   Size:        232      17     578     536    1088

  Fragments arranged by size: 

                1088     578     536     232      17
 
BsrGI T'GTAC_A

Cuts at:      0    2352    2451 
   Size:       2352      99
 
BssSI C'ACGA_G

Cuts at:      0     871    2451 
   Size:        871    1580
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     174    2153    2451 
   Size:        174    1979     298

  Fragments arranged by size: 

                1979     298     174
 
BstEII G'GTnAC_C

Cuts at:      0      45    1644    2451 
   Size:         45    1599     807

  Fragments arranged by size: 

                1599     807      45
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     705    1185    2316    2451 
   Size:        705     480    1131     135

  Fragments arranged by size: 

                1131     705     480     135
 
BstYI r'GATC_y

Cuts at:      0    1292    1957    2294    2451 
   Size:       1292     665     337     157

  Fragments arranged by size: 

                1292     665     337     157
 
Bsu36I CC'TnA_GG

Cuts at:      0    1079    2451 
   Size:       1079    1372
 
BtgI C'CryG_G

Cuts at:      0     515    2451 
   Size:        515    1936
 
BtsI GCAGTG_nn'

Cuts at:      0      15     372    2151    2451 
   Size:         15     357    1779     300

  Fragments arranged by size: 

                1779     357     300      15
 
DraIII CAC_nnn'GTG

Cuts at:      0    2025    2451 
   Size:       2025     426
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     100    2451 
   Size:        100    2351
 
EaeI y'GGCC_r

Cuts at:      0      56     234     316     926    1365    2451 
   Size:         56     178      82     610     439    1086

  Fragments arranged by size: 

                1086     610     439     178      82      56
 
EagI C'GGCC_G

Cuts at:      0     234    2451 
   Size:        234    2217
 
EarI CTCTTCn'nnn_

Cuts at:      0    1687    2366    2451 
   Size:       1687     679      85

  Fragments arranged by size: 

                1687     679      85
 
EcoICRI GAG'CTC

Cuts at:      0    1469    1717    2451 
   Size:       1469     248     734

  Fragments arranged by size: 

                1469     734     248
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     411     628     913    1421    2338    2451 
   Size:        411     217     285     508     917     113

  Fragments arranged by size: 

                 917     508     411     285     217     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0     619     694    1481    1505    1520    1521    2430    2451 
   Size:        619      75     787      24      15       1     909      21

  Fragments arranged by size: 

                 909     787     619      75      24      21      15       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1524    2221    2451 
   Size:       1524     697     230

  Fragments arranged by size: 

                1524     697     230
 
FseI GG_CCGG'CC

Cuts at:      0    1367    2451 
   Size:       1367    1084
 
FspI TGC'GCA

Cuts at:      0    1279    2451 
   Size:       1279    1172
 
HaeII r_GCGC'y

Cuts at:      0      72     140     607    2451 
   Size:         72      68     467    1844

  Fragments arranged by size: 

                1844     467      72      68
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1277    1309    1918    1950    2395    2427    2451 
   Size:       1277      32     609      32     445      32      24

  Fragments arranged by size: 

                1277     609     445      32      32      32      24
 
HincII GTy'rAC

Cuts at:      0     681    2451 
   Size:        681    1770
 
KasI G'GCGC_C

Cuts at:      0      68     136    2451 
   Size:         68      68    2315

  Fragments arranged by size: 

                2315      68      68
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     554    2451 
   Size:        554    1897
 
MscI TGG'CCA

Cuts at:      0     318     928    2451 
   Size:        318     610    1523

  Fragments arranged by size: 

                1523     610     318
 
MslI CAynn'nnrTG

Cuts at:      0    1381    2451 
   Size:       1381    1070
 
MspA1I CmG'CkG

Cuts at:      0     302     311     511    1258    1348    1553    2451 
   Size:        302       9     200     747      90     205     898

  Fragments arranged by size: 

                 898     747     302     205     200      90       9
 
NaeI GCC'GGC

Cuts at:      0    1365    2451 
   Size:       1365    1086
 
NarI GG'CG_CC

Cuts at:      0      69     137    2451 
   Size:         69      68    2314

  Fragments arranged by size: 

                2314      69      68
 
NcoI C'CATG_G

Cuts at:      0     515    2451 
   Size:        515    1936
 
NdeI CA'TA_TG

Cuts at:      0    1845    2277    2451 
   Size:       1845     432     174

  Fragments arranged by size: 

                1845     432     174
 
NgoMIV G'CCGG_C

Cuts at:      0    1363    2451 
   Size:       1363    1088
 
NspI r_CATG'y

Cuts at:      0     378     960    1122    1603    2451 
   Size:        378     582     162     481     848

  Fragments arranged by size: 

                 848     582     481     378     162
 
PciI A'CATG_T

Cuts at:      0     956    2451 
   Size:        956    1495
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     314    2025    2451 
   Size:        314    1711     426

  Fragments arranged by size: 

                1711     426     314
 
PpuMI rG'GwC_Cy

Cuts at:      0    1481    1505    2430    2451 
   Size:       1481      24     925      21

  Fragments arranged by size: 

                1481     925      24      21
 
PsiI TTA'TAA

Cuts at:      0    2381    2451 
   Size:       2381      70
 
PspOMI G'GGCC_C

Cuts at:      0     694    1520    1899    2451 
   Size:        694     826     379     552

  Fragments arranged by size: 

                 826     694     552     379
 
PspXI vC'TCGA_Gb

Cuts at:      0      73    2451 
   Size:         73    2378
 
PstI C_TGCA'G

Cuts at:      0     860    1347    2195    2451 
   Size:        860     487     848     256

  Fragments arranged by size: 

                 860     848     487     256
 
PvuII CAG'CTG

Cuts at:      0     311     511    1258    1348    1553    2451 
   Size:        311     200     747      90     205     898

  Fragments arranged by size: 

                 898     747     311     205     200      90
 
SacI G_AGCT'C

Cuts at:      0    1471    1719    2451 
   Size:       1471     248     732

  Fragments arranged by size: 

                1471     732     248
 
SanDI GG'GwC_CC

Cuts at:      0    1481    2430    2451 
   Size:       1481     949      21

  Fragments arranged by size: 

                1481     949      21
 
ScaI AGT'ACT

Cuts at:      0    2338    2451 
   Size:       2338     113
 
SfcI C'TryA_G

Cuts at:      0     159     814     856    1343    2191    2451 
   Size:        159     655      42     487     848     260

  Fragments arranged by size: 

                 848     655     487     260     159      42
 
SfoI GGC'GCC

Cuts at:      0      70     138    2451 
   Size:         70      68    2313

  Fragments arranged by size: 

                2313      70      68
 
SmaI CCC'GGG

Cuts at:      0     272    1781    2451 
   Size:        272    1509     670

  Fragments arranged by size: 

                1509     670     272
 
SmlI C'TyrA_G

Cuts at:      0      73     400    2451 
   Size:         73     327    2051

  Fragments arranged by size: 

                2051     327      73
 
StuI AGG'CCT

Cuts at:      0     704    1184    1884    2451 
   Size:        704     480     700     567

  Fragments arranged by size: 

                 704     700     567     480
 
StyI C'CwwG_G

Cuts at:      0     212     515     995    2451 
   Size:        212     303     480    1456

  Fragments arranged by size: 

                1456     480     303     212
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1928    2451 
   Size:       1928     523
 
TatI w'GTAC_w

Cuts at:      0    1391    2336    2352    2451 
   Size:       1391     945      16      99

  Fragments arranged by size: 

                1391     945      99      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1159    1191    1949    1981    2451 
   Size:       1159      32     758      32     470

  Fragments arranged by size: 

                1159     758     470      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1915    2451 
   Size:       1915     536
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1119    2451 
   Size:       1119    1332
 
XhoI C'TCGA_G

Cuts at:      0      73    2451 
   Size:         73    2378
 
XmaI C'CCGG_G

Cuts at:      0     270    1779    2451 
   Size:        270    1509     672

  Fragments arranged by size: 

                1509     672     270
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbeI    BbvCI
     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI
  Eco57MI EcoO109I     FalI     FseI     FspI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NspI     PciI    PflMI    PpuMI     PsiI   PspOMI
    PspXI     PstI    PvuII     SacI    SanDI     ScaI     SfcI     SfoI
     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AscI    AsiSI    AvrII     BaeI     BbsI     BcgI    BciVI
    BfrBI     BglI     BmtI     BplI    BsaAI    BsiWI    BsmBI    BspEI
    BsrBI   BssHII    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI
     EciI    EcoNI    EcoRI    EcoRV    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsrI     PvuI    RsrII
    SacII     SalI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI     XmnI
     ZraI
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