Restriction map for pF1KB4221
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
BamHI       BcgI        BciVI       BclI        BfrI        
BglII       BsaBI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstYI       BstZ17I     ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
FbaI        FseI        HindIII     HpaI        KspI        
MamI        MfeI        MflI        MluI        MseI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tru9I       Tsp509I     TthHB8I     
XbaI        XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI          172  GTCTAC               GT^[AC][GT]AC
AccII        1190  CGCG                 CG^CG
AclI          274  AACGTT               AA^CGTT
AcyI          211  GGCGCC               G[GA]^CG[CT]C
AlwI          835  GATCC                GGATC(4/5)
AocI         1177  CCTGAGG              CC^T[ACGT]AGG
AspI          261  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         374  TGCGCA               TGC^GCA
AvrII        1678  CCTAGG               C^CTAGG
BbeI          211  GGCGCC               GGCGC^C
BfaI         1679  CTAG                 C^TAG
BlnI         1678  CCTAGG               C^CTAGG
BlpI          790  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      790  GCTGAGC              GC^T[ACGT]AGC
BsaHI         211  GGCGCC               G[GA]^CG[CT]C
BsaI          259  GAGACC               GGTCTC(1/5)
BsgI           97  CTGCAC               GTGCAG(16/14)
BsmAI         259  GAGAC                GTCTC(1/5)
BsrBI         469  GAGCGG               CCGCTC(-3/-3)
BsrFI         603  ACCGGC               [GA]^CCGG[CT]
BstUI        1190  CGCG                 CG^CG
Bsu36I       1177  CCTGAGG              CC^T[ACGT]AGG
CelII         790  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        603  ACCGGC               [GA]^CCGG[CT]
Eco81I       1177  CCTGAGG              CC^T[ACGT]AGG
EcoRV         481  GATATC               GAT^ATC
EcoT22I        16  ATGCAT               ATGCA^T
FspI          374  TGCGCA               TGC^GCA
HaeII         211  GGCGCC               [GA]GCGC^[CT]
HgaI          170  GCGTC                GACGC(5/10)
Hin1I         211  GGCGCC               G[GA]^CG[CT]C
HincII       1375  GTCAAC               GT[CT]^[GA]AC
HindII       1375  GTCAAC               GT[CT]^[GA]AC
KasI          211  GGCGCC               G^GCGCC
MaeI         1679  CTAG                 C^TAG
MaeIII        992  GTGAC                ^GT[ACGT]AC
MvnI         1190  CGCG                 CG^CG
NarI          211  GGCGCC               GG^CGCC
NcoI         1184  CCATGG               C^CATGG
NsiI           16  ATGCAT               ATGCA^T
NspI          656  ACATGC               [GA]CATG^[CT]
PflFI         261  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         733  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I      274  AACGTT               AA^CGTT
PstI          121  CTGCAG               CTGCA^G
SfcI          121  CTGCAG               C^T[GA][CT]AG
SfoI          211  GGCGCC               GGC^GCC
Tsp45I        992  GTGAC                ^GT[GC]AC
Tth111I       261  GACCACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        733  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I        112  GGTACC               G^GTACC
              807  GGTACC              
AccIII        872  TCCGGA               T^CCGGA
             1460  TCCGGA              
ApaI          665  GGGCCC               GGGCC^C
              687  GGGCCC              
Asp718I       112  GGTACC               G^GTACC
              807  GGTACC              
BbrPI         318  CACGTG               CAC^GTG
             1621  CACGTG              
BbsI          514  GAAGAC               GAAGAC(2/6)
             1369  GTCTTC              
BbvCI         253  GCTGAGG              CCTCAGC(-5/-2)
              751  GCTGAGG             
BglI          570  GCCCGGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              961  GCCATCATGGC         
BpuAI         514  GAAGAC               GAAGAC(2/6)
             1369  GTCTTC              
BsaAI         318  CACGTG               [CT]AC^GT[GA]
             1621  CACGTG              
BsaWI         872  TCCGGA               [AT]^CCGG[AT]
             1460  TCCGGA              
BseAI         872  TCCGGA               T^CCGGA
             1460  TCCGGA              
BspEI         872  TCCGGA               T^CCGGA
             1460  TCCGGA              
EarI           10  GAAGAG               CTCTTC(1/4)
             1090  GAAGAG              
HphI          240  GGTGA                GGTGA(8/7)
             1246  TCACC               
KpnI          112  GGTACC               GGTAC^C
              807  GGTACC              
Ksp632I        10  GAAGAG               CTCTTC(1/4)
             1090  GAAGAG              
MroI          872  TCCGGA               T^CCGGA
             1460  TCCGGA              
MslI          526  CACGACGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1617  CACCCACGTG          
MspA1I        118  CAGCTG               C[AC]G^C[GT]G
              124  CAGCTG              
PmaCI         318  CACGTG               CAC^GTG
             1621  CACGTG              
PmlI          318  CACGTG               CAC^GTG
             1621  CACGTG              
PvuII         118  CAGCTG               CAG^CTG
              124  CAGCTG              
SmaI          662  CCCGGG               CCC^GGG
             1427  CCCGGG              
SmlI          580  CTTGAG               C^T[CT][GA]AG
             1000  CTCAAG              
XmaCI         662  CCCGGG               C^CCGGG
             1427  CCCGGG              
XmaI          662  CCCGGG               C^CCGGG
             1427  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   71 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MaeII       MboI        
MboII       MluNI       MnlI        MscI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TseI        
TspRI       
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