Restriction map for pF1KB4188
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BcgI        
BclI        BcnI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HindIII     HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

ApaI         1041  GGGCCC               GGGCC^C
Asp700I       574  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          569  CTCGAG               C^[CT]CG[GA]G
AvaII        1003  GGTCC                G^G[AT]CC
BbsI         1028  GAAGAC               GAAGAC(2/6)
BbvCI         448  GCTGAGG              CCTCAGC(-5/-2)
BciVI         802  GGATAC               GTATCC(6/5)
BglI          577  GCCTTTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          44  AGATCT               A^GATCT
BpmI          266  CTCCAG               CTGGAG(16/14)
BpuAI        1028  GAAGAC               GAAGAC(2/6)
BsaBI         468  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          263  GGTCTC               GGTCTC(1/5)
BsgI          622  GTGCAG               GTGCAG(16/14)
BsiYI         177  CCTGTTGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          177  CCTGTTGGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI           3  GTCCC                GGGAC(10/14)
BsmI          228  GAATGC               GAATGC(1/-1)
BsoBI         569  CTCGAG               C^[CT]CG[GA]G
BsrBI         823  GAGCGG               CCGCTC(-3/-3)
BsrDI         255  CATTGC               GCAATG(2/0)
BsrFI          13  ACCGGC               [GA]^CCGG[CT]
BstBI        1025  TTCGAA               TT^CGAA
BstYI          44  AGATCT               [GA]^GATC[CT]
Cac8I         969  GCATGC               GC[ACGT]^[ACGT]GC
Cfr10I         13  ACCGGC               [GA]^CCGG[CT]
EcoNI         177  CCTGTTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1022  GAATTC               G^AATTC
EcoT14I       710  CCAAGG               C^C[AT][AT]GG
HapII          14  CCGG                 C^CGG
HincII        927  GTTGAC               GT[CT]^[GA]AC
HindII        927  GTTGAC               GT[CT]^[GA]AC
HpaII          14  CCGG                 C^CGG
MaeII         515  ACGT                 A^CGT
MamI          468  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI           44  AGATCT               [GA]^GATC[CT]
MspI           14  CCGG                 C^CGG
NspV         1025  TTCGAA               TT^CGAA
PaeR7I        569  CTCGAG               C^TCGAG
PleI          772  GAGTC                GAGTC(4/5)
PpuMI        1002  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         446  CAGCTG               CAG^CTG
SbfI         1006  CCTGCAGG             CCTGCA^GG
SfuI         1025  TTCGAA               TT^CGAA
SmlI          569  CTCGAG               C^T[CT][GA]AG
SphI          969  GCATGC               GCATG^C
Sse8387I     1006  CCTGCAGG             CCTGCA^GG
StyI          710  CCAAGG               C^C[AT][AT]GG
Tsp45I        417  GTCAC                ^GT[GC]AC
TspRI         437  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoI          569  CTCGAG               C^TCGAG
XhoII          44  AGATCT               [GA]^GATC[CT]
XmnI          574  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          825  GCGG                 CCGC(-3/-1)
             1048  GCGG                
AcsI          331  GAATTT               [GA]^AATT[CT]
             1022  GAATTC              
AlwI          118  GGATC                GGATC(4/5)
              492  GGATC               
ApoI          331  GAATTT               [GA]^AATT[CT]
             1022  GAATTC              
BalI          342  TGGCCA               TGG^CCA
              662  TGGCCA              
BanII         483  GAGCCC               G[GA]GC[CT]^C
             1041  GGGCCC              
BfaI          839  CTAG                 C^TAG
              859  CTAG                
BmyI          483  GAGCCC               G[AGT]GC[ACT]^C
             1041  GGGCCC              
BsmAI         761  GAGAC                GTCTC(1/5)
              264  GTCTC               
Bsp1286I      483  GAGCCC               G[AGT]GC[ACT]^C
             1041  GGGCCC              
DraII        1002  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1040  GGGGCCC             
EaeI          342  TGGCCA               [CT]^GGCC[GA]
              662  TGGCCA              
EcoO109I     1002  AGGTCCT              [GA]G^G[ACGT]CC[CT]
             1040  GGGGCCC             
MaeI          839  CTAG                 C^TAG
              859  CTAG                
MaeIII        417  GTCAC                ^GT[ACGT]AC
              725  GTTAC               
MluNI         342  TGGCCA               TGG^CCA
              662  TGGCCA              
MscI          342  TGGCCA               TGG^CCA
              662  TGGCCA              
MslI          274  CATTCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              748  CATTCCCATG          
MspA1I        446  CAGCTG               C[AC]G^C[GT]G
             1046  CAGCGG              
NlaIV        1040  GGGGCC               GG[ACGT]^[ACGT]CC
             1041  GGGCCC              
NspI          808  ACATGC               [GA]CATG^[CT]
              969  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AfaI        AluI        BbvI        BsaJI       BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TaqI        TfiI        Tru9I       TseI        
Tsp509I     TthHB8I     
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