Restriction map for pF1KB4188
 (Linear) (Six-base) MAPSORT of: KIBB4188.seq  Check: 2314  from: 1  to: 1071



KIBB4188.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     343    1071 
   Size:        119     224     728

  Fragments arranged by size: 

                 728     224     119
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1004    1071 
   Size:       1004      67
 
ApaI G_GGCC'C

Cuts at:      0    1045    1071 
   Size:       1045      26
 
ApoI r'AATT_y

Cuts at:      0     331    1022    1071 
   Size:        331     691      49

  Fragments arranged by size: 

                 691     331      49
 
AvaI C'yCGr_G

Cuts at:      0     569    1071 
   Size:        569     502
 
BanII G_rGCy'C

Cuts at:      0     487    1045    1071 
   Size:        487     558      26

  Fragments arranged by size: 

                 558     487      26
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1035    1071 
   Size:       1035      36
 
BbvCI CC'TCA_GC

Cuts at:      0     449    1071 
   Size:        449     622
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     796    1071 
   Size:        796     275
 
BglI GCCn_nnn'nGGC

Cuts at:      0     583    1071 
   Size:        583     488
 
BglII A'GATC_T

Cuts at:      0      44    1071 
   Size:         44    1027
 
Bme1580I G_kGCm'C

Cuts at:      0    1045    1071 
   Size:       1045      26
 
BmgBI CAC'GTC

Cuts at:      0     516    1071 
   Size:        516     555
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251    1071 
   Size:        251     820
 
Bpu10I CC'TnA_GC

Cuts at:      0     449    1071 
   Size:        449     622
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     269    1071 
   Size:        269     802
 
BsaBI GATnn'nnATC

Cuts at:      0     472    1071 
   Size:        472     599
 
BseYI C'CCAG_C

Cuts at:      0    1044    1071 
   Size:       1044      27
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643    1071 
   Size:        643     428
 
BsmI GAATG_Cn'

Cuts at:      0     234    1071 
   Size:        234     837
 
Bsp1286I G_dGCh'C

Cuts at:      0     487    1045    1071 
   Size:        487     558      26

  Fragments arranged by size: 

                 558     487      26
 
BsrBI CCG'CTC

Cuts at:      0     825    1071 
   Size:        825     246
 
BsrDI GCAATG_nn'

Cuts at:      0     254    1071 
   Size:        254     817
 
BsrFI r'CCGG_y

Cuts at:      0      13    1071 
   Size:         13    1058
 
BstBI TT'CG_AA

Cuts at:      0    1026    1071 
   Size:       1026      45
 
BstYI r'GATC_y

Cuts at:      0      44    1071 
   Size:         44    1027
 
EaeI y'GGCC_r

Cuts at:      0     342     662    1071 
   Size:        342     320     409

  Fragments arranged by size: 

                 409     342     320
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     251     343    1071 
   Size:        119     132      92     728

  Fragments arranged by size: 

                 728     132     119      92
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     181    1071 
   Size:        181     890
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1003    1041    1071 
   Size:       1003      38      30

  Fragments arranged by size: 

                1003      38      30
 
EcoRI G'AATT_C

Cuts at:      0    1022    1071 
   Size:       1022      49
 
HincII GTy'rAC

Cuts at:      0     929    1071 
   Size:        929     142
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     608    1071 
   Size:        161     447     463

  Fragments arranged by size: 

                 463     447     161
 
MscI TGG'CCA

Cuts at:      0     344     664    1071 
   Size:        344     320     407

  Fragments arranged by size: 

                 407     344     320
 
MslI CAynn'nnrTG

Cuts at:      0     278     752    1071 
   Size:        278     474     319

  Fragments arranged by size: 

                 474     319     278
 
MspA1I CmG'CkG

Cuts at:      0     448    1048    1071 
   Size:        448     600      23

  Fragments arranged by size: 

                 600     448      23
 
NspI r_CATG'y

Cuts at:      0     812     973    1071 
   Size:        812     161      98

  Fragments arranged by size: 

                 812     161      98
 
PasI CC'CwG_GG

Cuts at:      0     234    1071 
   Size:        234     837
 
PpuMI rG'GwC_Cy

Cuts at:      0    1003    1071 
   Size:       1003      68
 
PsiI TTA'TAA

Cuts at:      0     655    1071 
   Size:        655     416
 
PspOMI G'GGCC_C

Cuts at:      0    1041    1071 
   Size:       1041      30
 
PstI C_TGCA'G

Cuts at:      0     447     936    1011    1071 
   Size:        447     489      75      60

  Fragments arranged by size: 

                 489     447      75      60
 
PvuII CAG'CTG

Cuts at:      0     448    1071 
   Size:        448     623
 
SbfI CC_TGCA'GG

Cuts at:      0    1011    1071 
   Size:       1011      60
 
SfcI C'TryA_G

Cuts at:      0     443     932    1007    1071 
   Size:        443     489      75      64

  Fragments arranged by size: 

                 489     443      75      64
 
SmlI C'TyrA_G

Cuts at:      0     569    1071 
   Size:        569     502
 
SphI G_CATG'C

Cuts at:      0     973    1071 
   Size:        973      98
 
StyI C'CwwG_G

Cuts at:      0     710    1071 
   Size:        710     361
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     508    1071 
   Size:        508     563
 
TatI w'GTAC_w

Cuts at:      0      71     954    1071 
   Size:         71     883     117

  Fragments arranged by size: 

                 883     117      71
 
XhoI C'TCGA_G

Cuts at:      0     569    1071 
   Size:        569     502
 
XmnI GAAnn'nnTTC

Cuts at:      0     578    1071 
   Size:        578     493
 

 Enzymes that do cut:

     AcuI     AlfI     ApaI     ApoI     AvaI    BanII     BbsI    BbvCI
    BciVI     BglI    BglII Bme1580I    BmgBI     BpmI   Bpu10I     BsaI
    BsaBI    BseYI     BsgI     BsmI Bsp1286I    BsrBI    BsrDI    BsrFI
    BstBI    BstYI     EaeI  Eco57MI    EcoNI EcoO109I    EcoRI   HincII
     MmeI     MscI     MslI   MspA1I     NspI     PasI    PpuMI     PsiI
   PspOMI     PstI    PvuII     SbfI     SfcI     SmlI     SphI     StyI
    TaqII     TatI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI     BclI
    BfrBI     BlpI     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaHI
    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI
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