Restriction map for pF1KB4173
 (Linear) (Six-base) MAPSORT of: KIBB4173.seq  Check: 8614  from: 1  to: 2856



KIBB4173.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2376    2856 
   Size:       2376     480
 
AatII G_ACGT'C

Cuts at:      0     251    1199    1937    2856 
   Size:        251     948     738     919

  Fragments arranged by size: 

                 948     919     738     251
 
AccI GT'mk_AC

Cuts at:      0     994    1016    2856 
   Size:        994      22    1840

  Fragments arranged by size: 

                1840     994      22
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     721    2043    2856 
   Size:        721    1322     813

  Fragments arranged by size: 

                1322     813     721
 
AflIII A'CryG_T

Cuts at:      0    1847    2670    2856 
   Size:       1847     823     186

  Fragments arranged by size: 

                1847     823     186
 
AhdI GACnn_n'nnGTC

Cuts at:      0     912    2856 
   Size:        912    1944
 
AleI CACnn'nnGTG

Cuts at:      0     703    2260    2653    2856 
   Size:        703    1557     393     203

  Fragments arranged by size: 

                1557     703     393     203
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     409     441    1228    1260    1549    1581    2856 
   Size:        409      32     787      32     289      32    1275

  Fragments arranged by size: 

                1275     787     409     289      32      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     348    1062    1290    1425    2232    2856 
   Size:        348     714     228     135     807     624

  Fragments arranged by size: 

                 807     714     624     348     228     135
 
ApaI G_GGCC'C

Cuts at:      0     194    1033    1182    1351    2485    2856 
   Size:        194     839     149     169    1134     371

  Fragments arranged by size: 

                1134     839     371     194     169     149
 
ApaLI G'TGCA_C

Cuts at:      0     664    2856 
   Size:        664    2192
 
AvaI C'yCGr_G

Cuts at:      0     116     194    1344    1444    1769    2558    2856 
   Size:        116      78    1150     100     325     789     298

  Fragments arranged by size: 

                1150     789     325     298     116     100      78
 
BamHI G'GATC_C

Cuts at:      0    1004    1326    2856 
   Size:       1004     322    1530

  Fragments arranged by size: 

                1530    1004     322
 
BanI G'GyrC_C

Cuts at:      0      11     576     609     708    1527    1914    2331    2733 
   Size:         11     565      33      99     819     387     417     402

Cuts at:   2733    2856 
   Size:        123

  Fragments arranged by size: 

                 819     565     417     402     387     123      99      33
                  11
 
BanII G_rGCy'C

Cuts at:      0     194     360     427     650     976    1033    1040    1182 
   Size:        194     166      67     223     326      57       7     142

Cuts at:   1182    1351    1585    1858    2485    2856 
   Size:        169     234     273     627     371

  Fragments arranged by size: 

                 627     371     326     273     234     223     194     169
                 166     142      67      57       7
 
BbeI G_GCGC'C

Cuts at:      0     712    2737    2856 
   Size:        712    2025     119

  Fragments arranged by size: 

                2025     712     119
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1069    2856 
   Size:       1069    1787
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1127    2077    2278    2856 
   Size:       1127     950     201     578

  Fragments arranged by size: 

                1127     950     578     201
 
BlpI GC'TnA_GC

Cuts at:      0     805    2856 
   Size:        805    2051
 
Bme1580I G_kGCm'C

Cuts at:      0      86     194     236     581     612     668    1033    1182 
   Size:         86     108      42     345      31      56     365     149

Cuts at:   1182    1351    1532    2311    2485    2856 
   Size:        169     181     779     174     371

  Fragments arranged by size: 

                 779     371     365     345     181     174     169     149
                 108      86      56      42      31
 
BmgBI CAC'GTC

Cuts at:      0     510    1848    2151    2856 
   Size:        510    1338     303     705

  Fragments arranged by size: 

                1338     705     510     303
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     133    1107    2254    2856 
   Size:        133     974    1147     602

  Fragments arranged by size: 

                1147     974     602     133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     433     922    1008    1633    1639    1662    2206 
   Size:        166     267     489      86     625       6      23     544

Cuts at:   2206    2251    2623    2856 
   Size:         45     372     233

  Fragments arranged by size: 

                 625     544     489     372     267     233     166      86
                  45      23       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     421     982    2856 
   Size:        421     561    1874

  Fragments arranged by size: 

                1874     561     421
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     580     884    1405    2155    2856 
   Size:        580     304     521     750     701

  Fragments arranged by size: 

                 750     701     580     521     304
 
BsaAI yAC'GTr

Cuts at:      0    1629    2671    2856 
   Size:       1629    1042     185

  Fragments arranged by size: 

                1629    1042     185
 
BsaHI Gr'CG_yC

Cuts at:      0     248     709    1196    1934    2734    2856 
   Size:        248     461     487     738     800     122

  Fragments arranged by size: 

                 800     738     487     461     248     122
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     409     439    1210    1240    2856 
   Size:        409      30     771      30    1616

  Fragments arranged by size: 

                1616     771     409      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     123     163     417     658    1125    1866    1978    2104 
   Size:        123      40     254     241     467     741     112     126

Cuts at:   2104    2856 
   Size:        752

  Fragments arranged by size: 

                 752     741     467     254     241     126     123     112
                  40
 
BseYI C'CCAG_C

Cuts at:      0     159     391     400    1124    1908    1985    1989    2072 
   Size:        159     232       9     724     784      77       4      83

Cuts at:   2072    2458    2738    2856 
   Size:        386     280     118

  Fragments arranged by size: 

                 784     724     386     280     232     159     118      83
                  77       9       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56    1072    1276    1303    1696    2856 
   Size:         56    1016     204      27     393    1160

  Fragments arranged by size: 

                1160    1016     393     204      56      27
 
BsiEI CG_ry'CG

Cuts at:      0     219     684    1305    1821    2654    2856 
   Size:        219     465     621     516     833     202

  Fragments arranged by size: 

                 833     621     516     465     219     202
 
BsiHKAI G_wGCw'C

Cuts at:      0     427     668     788    1858    2267    2856 
   Size:        427     241     120    1070     409     589

  Fragments arranged by size: 

                1070     589     427     409     241     120
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     194     236     360     427     581     612     650 
   Size:         86     108      42     124      67     154      31      38

Cuts at:    650     668     788     976    1033    1040    1182    1351    1532 
   Size:         18     120     188      57       7     142     169     181

Cuts at:   1532    1585    1858    2267    2311    2485    2856 
   Size:         53     273     409      44     174     371

  Fragments arranged by size: 

                 409     371     273     188     181     174     169     154
                 142     124     120     108      86      67      57      53
                  44      42      38      31      18       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1718    1913    2330    2376    2733    2856 
   Size:       1718     195     417      46     357     123

  Fragments arranged by size: 

                1718     417     357     195     123      46
 
BsrBI CCG'CTC

Cuts at:      0    1799    2856 
   Size:       1799    1057
 
BsrDI GCAATG_nn'

Cuts at:      0     951    2856 
   Size:        951    1905
 
BsrFI r'CCGG_y

Cuts at:      0      29     668    1304    1414    1828    2067    2421    2856 
   Size:         29     639     636     110     414     239     354     435

  Fragments arranged by size: 

                 639     636     435     414     354     239     110      29
            
 
BssSI C'ACGA_G

Cuts at:      0      52    2856 
   Size:         52    2804
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      70     348    1290    2331    2856 
   Size:         70     278     942    1041     525

  Fragments arranged by size: 

                1041     942     525     278      70
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     957    1047    1485    2526    2802    2856 
   Size:        957      90     438    1041     276      54

  Fragments arranged by size: 

                1041     957     438     276      90      54
 
BstYI r'GATC_y

Cuts at:      0     552    1004    1326    2856 
   Size:        552     452     322    1530

  Fragments arranged by size: 

                1530     552     452     322
 
BtgI C'CryG_G

Cuts at:      0     315     950    1277    1815    2165    2519    2729    2856 
   Size:        315     635     327     538     350     354     210     127

  Fragments arranged by size: 

                 635     538     354     350     327     315     210     127
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1725    2856 
   Size:       1725    1131
 
BtsI GCAGTG_nn'

Cuts at:      0     236    1451    2413    2856 
   Size:        236    1215     962     443

  Fragments arranged by size: 

                1215     962     443     236
 
DraIII CAC_nnn'GTG

Cuts at:      0     242     833     927    2856 
   Size:        242     591      94    1929

  Fragments arranged by size: 

                1929     591     242      94
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2797    2812    2856 
   Size:       2797      15      44

  Fragments arranged by size: 

                2797      44      15
 
EaeI y'GGCC_r

Cuts at:      0      38     216     446     681    1274    1302    1416    1661 
   Size:         38     178     230     235     593      28     114     245

Cuts at:   1661    1812    1818    1830    2636    2726    2856 
   Size:        151       6      12     806      90     130

  Fragments arranged by size: 

                 806     593     245     235     230     178     151     130
                 114      90      38      28      12       6
 
EagI C'GGCC_G

Cuts at:      0     216     681    1302    1818    2856 
   Size:        216     465     621     516    1038

  Fragments arranged by size: 

                1038     621     516     465     216
 
EarI CTCTTCn'nnn_

Cuts at:      0    2185    2856 
   Size:       2185     671
 
EcoICRI GAG'CTC

Cuts at:      0     425    1856    2856 
   Size:        425    1431    1000

  Fragments arranged by size: 

                1431    1000     425
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     433     721     922    1008    1633    1639    1662 
   Size:        166     267     288     201      86     625       6      23

Cuts at:   1662    2043    2206    2251    2623    2856 
   Size:        381     163      45     372     233

  Fragments arranged by size: 

                 625     381     372     288     267     233     201     166
                 163      86      45      23       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1749    2542    2856 
   Size:       1749     793     314

  Fragments arranged by size: 

                1749     793     314
 
EcoO109I rG'GnC_Cy

Cuts at:      0     190     191     843    1101    1178    1348    1367    1649 
   Size:        190       1     652     258      77     170      19     282

Cuts at:   1649    1994    2091    2244    2343    2481    2482    2612    2745 
   Size:        345      97     153      99     138       1     130     133

Cuts at:   2745    2799    2856 
   Size:         54      57

  Fragments arranged by size: 

                 652     345     282     258     190     170     153     138
                 133     130      99      97      77      57      54      19
                   1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1275    2856 
   Size:       1275    1581
 
HaeII r_GCGC'y

Cuts at:      0     570     712     901    1311    1759    2737    2856 
   Size:        570     142     189     410     448     978     119

  Fragments arranged by size: 

                 978     570     448     410     189     142     119
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     537     569     898     930    1311    1343    2856 
   Size:        537      32     329      32     381      32    1513

  Fragments arranged by size: 

                1513     537     381     329      32      32      32
 
HincII GTy'rAC

Cuts at:      0     995    2856 
   Size:        995    1861
 
KasI G'GCGC_C

Cuts at:      0     708    2733    2856 
   Size:        708    2025     123

  Fragments arranged by size: 

                2025     708     123
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1592    2777    2795    2856 
   Size:       1592    1185      18      61

  Fragments arranged by size: 

                1592    1185      61      18
 
MscI TGG'CCA

Cuts at:      0    1814    2638    2728    2856 
   Size:       1814     824      90     128

  Fragments arranged by size: 

                1814     824     128      90
 
MslI CAynn'nnrTG

Cuts at:      0     703     955    2260    2653    2856 
   Size:        703     252    1305     393     203

  Fragments arranged by size: 

                1305     703     393     252     203
 
MspA1I CmG'CkG

Cuts at:      0      70     317     675     741     815    1124    1425    1989 
   Size:         70     247     358      66      74     309     301     564

Cuts at:   1989    2700    2849    2856 
   Size:        711     149       7

  Fragments arranged by size: 

                 711     564     358     309     301     247     149      74
                  70      66       7
 
NaeI GCC'GGC

Cuts at:      0    1306    1830    2069    2423    2856 
   Size:       1306     524     239     354     433

  Fragments arranged by size: 

                1306     524     433     354     239
 
NarI GG'CG_CC

Cuts at:      0     709    2734    2856 
   Size:        709    2025     122

  Fragments arranged by size: 

                2025     709     122
 
NcoI C'CATG_G

Cuts at:      0     950    1277    2519    2856 
   Size:        950     327    1242     337

  Fragments arranged by size: 

                1242     950     337     327
 
NgoMIV G'CCGG_C

Cuts at:      0    1304    1828    2067    2421    2856 
   Size:       1304     524     239     354     435

  Fragments arranged by size: 

                1304     524     435     354     239
 
NspI r_CATG'y

Cuts at:      0    1545    2856 
   Size:       1545    1311
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1161    2856 
   Size:       1161    1695
 
PfoI T'CCnGG_A

Cuts at:      0     143    1007    1228    1262    2183    2801    2856 
   Size:        143     864     221      34     921     618      55

  Fragments arranged by size: 

                 921     864     618     221     143      55      34
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     409     441    2856 
   Size:        409      32    2415

  Fragments arranged by size: 

                2415     409      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     843    1649    2745    2799    2856 
   Size:        843     806    1096      54      57

  Fragments arranged by size: 

                1096     843     806      57      54
 
PshAI GACnn'nnGTC

Cuts at:      0    1379    2856 
   Size:       1379    1477
 
PspOMI G'GGCC_C

Cuts at:      0     190    1029    1178    1347    2481    2856 
   Size:        190     839     149     169    1134     375

  Fragments arranged by size: 

                1134     839     375     190     169     149
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1609    1641    2856 
   Size:       1609      32    1215

  Fragments arranged by size: 

                1609    1215      32
 
PstI C_TGCA'G

Cuts at:      0     353     968    1898    2327    2373    2480    2699    2705 
   Size:        353     615     930     429      46     107     219       6

Cuts at:   2705    2856 
   Size:        151

  Fragments arranged by size: 

                 930     615     429     353     219     151     107      46
                   6
 
PvuI CG_AT'CG

Cuts at:      0    2654    2856 
   Size:       2654     202
 
PvuII CAG'CTG

Cuts at:      0     675     741     815    1989    2700    2849    2856 
   Size:        675      66      74    1174     711     149       7

  Fragments arranged by size: 

                1174     711     675     149      74      66       7
 
SacI G_AGCT'C

Cuts at:      0     427    1858    2856 
   Size:        427    1431     998

  Fragments arranged by size: 

                1431     998     427
 
SacII CC_GC'GG

Cuts at:      0     318    2856 
   Size:        318    2538
 
SalI G'TCGA_C

Cuts at:      0     993    2856 
   Size:        993    1863
 
SanDI GG'GwC_CC

Cuts at:      0    1649    2856 
   Size:       1649    1207
 
SbfI CC_TGCA'GG

Cuts at:      0    2480    2856 
   Size:       2480     376
 
SexAI A'CCwGG_T

Cuts at:      0    1523    2856 
   Size:       1523    1333
 
SfcI C'TryA_G

Cuts at:      0     349     624     964    1362    1894    2323    2369    2476 
   Size:        349     275     340     398     532     429      46     107

Cuts at:   2476    2695    2701    2781    2856 
   Size:        219       6      80      75

  Fragments arranged by size: 

                 532     429     398     349     340     275     219     107
                  80      75      46       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    2077    2278    2856 
   Size:       2077     201     578

  Fragments arranged by size: 

                2077     578     201
 
SfoI GGC'GCC

Cuts at:      0     710    2735    2856 
   Size:        710    2025     121

  Fragments arranged by size: 

                2025     710     121
 
SmaI CCC'GGG

Cuts at:      0     196    1446    2856 
   Size:        196    1250    1410

  Fragments arranged by size: 

                1410    1250     196
 
StuI AGG'CCT

Cuts at:      0     776    2856 
   Size:        776    2080
 
StyI C'CwwG_G

Cuts at:      0      59     482     581     950    1277    2195    2303    2346 
   Size:         59     423      99     369     327     918     108      43

Cuts at:   2346    2519    2543    2795    2856 
   Size:        173      24     252      61

  Fragments arranged by size: 

                 918     423     369     327     252     173     108      99
                  61      59      43      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     173     310     405    1747    2400    2856 
   Size:        173     137      95    1342     653     456

  Fragments arranged by size: 

                1342     653     456     173     137      95
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     408    1426    1608    2455    2856 
   Size:        408    1018     182     847     401

  Fragments arranged by size: 

                1018     847     408     401     182
 
XmaI C'CCGG_G

Cuts at:      0     194    1444    2856 
   Size:        194    1250    1412

  Fragments arranged by size: 

                1412    1250     194
 
ZraI GAC'GTC

Cuts at:      0     249    1197    1935    2856 
   Size:        249     948     738     921

  Fragments arranged by size: 

                 948     921     738     249
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AhdI     AleI     AloI
    AlwNI     ApaI    ApaLI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
    Hin4I   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI    PflMI     PfoI     PpiI    PpuMI
    PshAI   PspOMI     PsrI     PstI     PvuI    PvuII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI     SmaI
     StuI     StyI    TaqII     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AlfI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbsI    BbvCI     BcgI     BclI
    BfrBI    BglII     BmtI     BplI    BpuEI    BsaBI    BsaWI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI     DraI     EciI    EcoRI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PmeI     PmlI     PsiI    PspXI    RsrII     SapI     ScaI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
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