Restriction map for pF1KB4150
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# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AciI        
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MspI        
MunI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        Sau96I      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         223  CGCG                 CG^CG
AfaI          210  GTAC                 GT^AC
AflIII        185  ACATGT               A^C[GA][CT]GT
BamHI         270  GGATCC               G^GATCC
BanII         140  GAGCCC               G[GA]GC[CT]^C
BmyI          140  GAGCCC               G[AGT]GC[ACT]^C
BpmI           95  CTCCAG               CTGGAG(16/14)
BsiYI         333  CCAGCTGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          333  CCAGCTGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         130  GAGAC                GTCTC(1/5)
Bsp1286I      140  GAGCCC               G[AGT]GC[ACT]^C
BspLU11I      185  ACATGT               A^CATGT
BssKI         292  CCAGG                ^CC[ACGT]GG
BstF5I        266  GGATG                GGATG(2/0)
BstNI         292  CCAGG                CC^[AT]GG
BstUI         223  CGCG                 CG^CG
BstXI         319  CCAGAACAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         270  GGATCC               [GA]^GATC[CT]
Cac8I         332  GCCAGC               GC[ACGT]^[ACGT]GC
DpnII         271  GATC                 ^GATC
EarI           33  CTCTTC               CTCTTC(1/4)
EcoRII        292  CCAGG                ^CC[AT]GG
FokI          266  GGATG                GGATG(9/13)
HincII         61  GTTGAC               GT[CT]^[GA]AC
HindII         61  GTTGAC               GT[CT]^[GA]AC
HinfI          46  GATTC                G^A[ACGT]TC
Ksp632I        33  CTCTTC               CTCTTC(1/4)
MaeII         212  ACGT                 A^CGT
MboI          271  GATC                 ^GATC
MflI          270  GGATCC               [GA]^GATC[CT]
MspA1I        334  CAGCTG               C[AC]G^C[GT]G
MvaI          292  CCAGG                CC^[AT]GG
MvnI          223  CGCG                 CG^CG
NdeII         271  GATC                 ^GATC
NlaIII        186  CATG                 CATG^
NruI          222  TCGCGA               TCG^CGA
NspI          185  ACATGT               [GA]CATG^[CT]
PvuII         334  CAGCTG               CAG^CTG
RsaI          210  GTAC                 GT^AC
SapI           32  GCTCTTC              GCTCTTC(1/4)
Sau3AI        271  GATC                 ^GATC
ScrFI         292  CCAGG                CC^[ACGT]GG
SfaNI         364  GATGC                GCATC(5/9)
SspI          260  AATATT               AAT^ATT
TaqI          136  TCGA                 T^CGA
TfiI           46  GATTC                G^A[AT]TC
Tsp509I       246  AATT                 ^AATT
TthHB8I       136  TCGA                 T^CGA
XhoII         270  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AluI           31  AGCT                 AG^CT
              335  AGCT                
AlwI          271  GATCC                GGATC(4/5)
              270  GGATC               
DraI          229  TTTAAA               TTT^AAA
              312  TTTAAA              
HphI          127  GGTGA                GGTGA(8/7)
              280  GGTGA               
MwoI           32  GCTCTTCTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              164  GCAGCAGCAGC         
NlaIV         270  GGATCC               GG[ACGT]^[ACGT]CC
              329  GGAGCC              
TspRI          42  CAGTG                CA[GC]TG[ACGT][ACGT]^
               54  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

BbvI        Fnu4HI      ItaI        MboII       MnlI        
MseI        Tru9I       TseI        
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