Restriction map for pF1KB4115
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccII       AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AvaI        
AviII       BamHI       BbeI        BbrPI       BbvCI       
BfrI        BlpI        BpmI        Bpu1102I    BsaAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstUI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT22I     FseI        FspI        HaeII       HpaI        
KasI        KpnI        KspI        MluI        MvnI        
NarI        NdeI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PvuI        RcaI        RsrII       SacI        
SacII       SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SphI        Sse8387I    SspBI       SspI        SwaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         1154  GTCGAC               GT^[AC][GT]AC
AccIII       1841  TCCGGA               T^CCGGA
AclI          253  AACGTT               AA^CGTT
AcyI         1469  GGCGTC               G[GA]^CG[CT]C
AflIII       1866  ACACGT               A^C[GA][CT]GT
AspI         1834  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1971  CCTAGG               C^CTAGG
BanI         2320  GGTGCC               G^G[CT][GA]CC
BbsI          532  GAAGAC               GAAGAC(2/6)
BcgI          795  GCACCAGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2527  GGATAC               GTATCC(6/5)
BclI          609  TGATCA               T^GATCA
BglI          112  GCCTTTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1971  CCTAGG               C^CTAGG
BpuAI         532  GAAGAC               GAAGAC(2/6)
BsaHI        1469  GGCGTC               G[GA]^CG[CT]C
BsaI          726  GGTCTC               GGTCTC(1/5)
BsaWI        1841  TCCGGA               [AT]^CCGG[AT]
BseAI        1841  TCCGGA               T^CCGGA
BspEI        1841  TCCGGA               T^CCGGA
BsrFI        2606  GCCGGC               [GA]^CCGG[CT]
BssSI        1727  CACGAG               CACGAG(-5/-1)
BstEII       1831  GGTGACC              G^GT[ACGT]ACC
BstPI        1831  GGTGACC              G^GT[ACGT]ACC
BstXI        2517  CCATGGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         2600  GCGC                 GCG^C
Cfr10I       2606  GCCGGC               [GA]^CCGG[CT]
DraII        1112  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I     1112  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1831  GGTGACC              G^GT[ACGT]ACC
EcoRI        2211  GAATTC               G^AATTC
FbaI          609  TGATCA               T^GATCA
HgaI         1470  GCGTC                GACGC(5/10)
HhaI         2600  GCGC                 GCG^C
Hin1I        1469  GGCGTC               G[GA]^CG[CT]C
HinP1I       2600  GCGC                 G^CGC
MroI         1841  TCCGGA               T^CCGGA
MspA1I       2756  CAGCTG               C[AC]G^C[GT]G
NaeI         2606  GCCGGC               GCC^GGC
NgoMIV       2606  GCCGGC               G^CCGGC
NheI         1539  GCTAGC               G^CTAGC
PflFI        1834  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I      253  AACGTT               AA^CGTT
PstI          911  CTGCAG               CTGCA^G
PvuII        2756  CAGCTG               CAG^CTG
SalI         1154  GTCGAC               G^TCGAC
SfaNI        2285  GCATC                GCATC(5/9)
SpeI         2194  ACTAGT               A^CTAGT
Tth111I      1834  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          540  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI         1480  GAATTT               [GA]^AATT[CT]
             2211  GAATTC              
ApoI         1480  GAATTT               [GA]^AATT[CT]
             2211  GAATTC              
BglII         699  AGATCT               A^GATCT
             1887  AGATCT              
BsmI         1902  GAATGC               GAATGC(1/-1)
              352  GCATTC              
BsrBI        1772  GAGCGG               CCGCTC(-3/-3)
             1984  GAGCGG              
BsrDI        1894  GCAATG               GCAATG(2/0)
             2092  GCAATG              
FauI          195  GCGGG                CCCGC(4/6)
             1774  GCGGG               
HincII       1154  GTCGAC               GT[CT]^[GA]AC
             2359  GTCAAC              
HindII       1154  GTCGAC               GT[CT]^[GA]AC
             2359  GTCAAC              
HindIII       473  AAGCTT               A^AGCTT
             2663  AAGCTT              
MfeI          566  CAATTG               C^AATTG
              980  CAATTG              
MunI          566  CAATTG               C^AATTG
              980  CAATTG              
NcoI         1584  CCATGG               C^CATGG
             2517  CCATGG              
SexAI         966  ACCAGGT              A^CC[AT]GGT
             1835  ACCTGGT             
SfcI          911  CTGCAG               C^T[GA][CT]AG
             1977  CTACAG              
StuI         1968  AGGCCT               AGG^CCT
             2048  AGGCCT              
XcmI          105  CCACAGTGCCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2063  CCACAGGAGCTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BalI        BanII       BbvI        BcnI        BfaI        
BmyI        BsaBI       BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DrdI        
DsaI        EaeI        EarI        EcoNI       EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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