Restriction map for
pF1KB4092
(Linear) (Six-base) MAPSORT of: KIBB4092.seq Check: 176 from: 1 to: 1245
KIBB4092.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 112 1245
Size: 112 1133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1245
Size: 616 629
AgeI A'CCGG_T
Cuts at: 0 239 1245
Size: 239 1006
AlwNI CAG_nnn'CTG
Cuts at: 0 797 1245
Size: 797 448
ApaI G_GGCC'C
Cuts at: 0 13 626 1245
Size: 13 613 619
Fragments arranged by size:
619 613 13
ApoI r'AATT_y
Cuts at: 0 1083 1245
Size: 1083 162
AvaI C'yCGr_G
Cuts at: 0 637 1245
Size: 637 608
BanI G'GyrC_C
Cuts at: 0 700 1245
Size: 700 545
BanII G_rGCy'C
Cuts at: 0 13 626 1245
Size: 13 613 619
Fragments arranged by size:
619 613 13
BbvCI CC'TCA_GC
Cuts at: 0 790 1245
Size: 790 455
Bme1580I G_kGCm'C
Cuts at: 0 13 533 626 1245
Size: 13 520 93 619
Fragments arranged by size:
619 520 93 13
BmgBI CAC'GTC
Cuts at: 0 78 1245
Size: 78 1167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1195 1245
Size: 1195 50
Bpu10I CC'TnA_GC
Cuts at: 0 790 1245
Size: 790 455
BsaI GGTCTCn'nnnn_
Cuts at: 0 779 1245
Size: 779 466
BsaWI w'CCGG_w
Cuts at: 0 239 805 1245
Size: 239 566 440
Fragments arranged by size:
566 440 239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 787 817 1168 1198 1245
Size: 787 30 351 30 47
Fragments arranged by size:
787 351 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 745 920 1245
Size: 745 175 325
Fragments arranged by size:
745 325 175
BseYI C'CCAG_C
Cuts at: 0 6 1245
Size: 6 1239
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 100 490 631 868 1245
Size: 84 16 390 141 237 377
Fragments arranged by size:
390 377 237 141 84 16
BsiEI CG_ry'CG
Cuts at: 0 902 1245
Size: 902 343
BsiHKAI G_wGCw'C
Cuts at: 0 856 1245
Size: 856 389
Bsp1286I G_dGCh'C
Cuts at: 0 13 533 626 856 1245
Size: 13 520 93 230 389
Fragments arranged by size:
520 389 230 93 13
BspEI T'CCGG_A
Cuts at: 0 805 1245
Size: 805 440
BspHI T'CATG_A
Cuts at: 0 108 1011 1245
Size: 108 903 234
Fragments arranged by size:
903 234 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 112 1245
Size: 112 1133
BsrDI GCAATG_nn'
Cuts at: 0 424 1110 1245
Size: 424 686 135
Fragments arranged by size:
686 424 135
BsrFI r'CCGG_y
Cuts at: 0 239 632 1207 1245
Size: 239 393 575 38
Fragments arranged by size:
575 393 239 38
BstYI r'GATC_y
Cuts at: 0 585 1245
Size: 585 660
Bsu36I CC'TnA_GG
Cuts at: 0 14 1245
Size: 14 1231
BtgI C'CryG_G
Cuts at: 0 45 1245
Size: 45 1200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 27 62 1245
Size: 27 35 1183
Fragments arranged by size:
1183 35 27
EaeI y'GGCC_r
Cuts at: 0 616 1245
Size: 616 629
EarI CTCTTCn'nnn_
Cuts at: 0 969 1081 1245
Size: 969 112 164
Fragments arranged by size:
969 164 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 1195 1245
Size: 616 579 50
Fragments arranged by size:
616 579 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 17 1245
Size: 17 1228
EcoO109I rG'GnC_Cy
Cuts at: 0 10 622 623 882 1245
Size: 10 612 1 259 363
Fragments arranged by size:
612 363 259 10 1
EcoRI G'AATT_C
Cuts at: 0 1083 1245
Size: 1083 162
HaeII r_GCGC'y
Cuts at: 0 323 1245
Size: 323 922
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1245
Size: 1168 32 45
Fragments arranged by size:
1168 45 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1245
Size: 380 865
MslI CAynn'nnrTG
Cuts at: 0 250 360 544 607 1141 1245
Size: 250 110 184 63 534 104
Fragments arranged by size:
534 250 184 110 104 63
MspA1I CmG'CkG
Cuts at: 0 615 1245
Size: 615 630
NaeI GCC'GGC
Cuts at: 0 1209 1245
Size: 1209 36
NgoMIV G'CCGG_C
Cuts at: 0 1207 1245
Size: 1207 38
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 1245
Size: 76 32 1137
Fragments arranged by size:
1137 76 32
PspOMI G'GGCC_C
Cuts at: 0 9 622 1245
Size: 9 613 623
Fragments arranged by size:
623 613 9
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 898 930 1245
Size: 898 32 315
Fragments arranged by size:
898 315 32
SfcI C'TryA_G
Cuts at: 0 705 1212 1245
Size: 705 507 33
Fragments arranged by size:
705 507 33
SmaI CCC'GGG
Cuts at: 0 639 1245
Size: 639 606
StuI AGG'CCT
Cuts at: 0 680 696 1245
Size: 680 16 549
Fragments arranged by size:
680 549 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 916 1222 1245
Size: 273 643 306 23
Fragments arranged by size:
643 306 273 23
TatI w'GTAC_w
Cuts at: 0 734 1245
Size: 734 511
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 958 1189 1245
Size: 958 231 56
Fragments arranged by size:
958 231 56
XmaI C'CCGG_G
Cuts at: 0 637 1245
Size: 637 608
XmnI GAAnn'nnTTC
Cuts at: 0 380 1245
Size: 380 865
Enzymes that do cut:
AarI AcuI AgeI AlwNI ApaI ApoI AvaI BanI
BanII BbvCI Bme1580I BmgBI BpmI Bpu10I BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BstYI Bsu36I BtgI CspCI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
MmeI MslI MspA1I NaeI NgoMIV PpiI PspOMI PsrI
SfcI SmaI StuI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsiWI BsmI BsmBI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI BtsI
ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TstI
Tth111I XbaI XhoI ZraI