Restriction map for pF1KB4082
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        Alw44I      AlwNI       ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BglII       BsaBI       BsaI        
BseAI       BsiEI       BsiWI       BsmBI       BspEI       
BsrBI       BsrFI       BssHII      BstBI       BstEII      
BstPI       BstUI       Cfr10I      CpoI        DraI        
DraIII      DsaI        EagI        EarI        EclXI       
Eco52I      EcoNI       EcoO65I     EcoRV       FauI        
FseI        FspI        HpaI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII         592  GACGTC               GACGT^C
AcyI          592  GACGTC               G[GA]^CG[CT]C
AhdI          316  GACCAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          969  CCTGAGG              CC^T[ACGT]AGG
ApaI          353  GGGCCC               GGGCC^C
Asp700I       198  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         316  GACCAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         705  CCTAGG               C^CTAGG
BanI         1840  GGTGCC               G^G[CT][GA]CC
BclI         1925  TGATCA               T^GATCA
BlnI          705  CCTAGG               C^CTAGG
BsaAI         220  CACGTA               [CT]AC^GT[GA]
BsaHI         592  GACGTC               G[GA]^CG[CT]C
BsaWI        2050  TCCGGT               [AT]^CCGG[AT]
BseRI         972  GAGGAG               GAGGAG(10/8)
BsmI          897  GAATGC               GAATGC(1/-1)
Bsp1407I     1310  TGTACA               T^GTACA
BspDI        1147  ATCGAT               AT^CGAT
BspLU11I     1392  ACATGT               A^CATGT
BspMI        1721  GCAGGT               ACCTGC(4/8)
BsrGI        1310  TGTACA               T^GTACA
BssSI        1769  CTCGTG               CACGAG(-5/-1)
Bst1107I      912  GTATAC               GTA^TAC
BstXI        2513  CCATGATGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       912  GTATAC               GTA^TAC
Bsu36I        969  CCTGAGG              CC^T[ACGT]AGG
ClaI         1147  ATCGAT               AT^CGAT
DrdI          658  GACTGCGAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      316  GACCAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        969  CCTGAGG              CC^T[ACGT]AGG
EcoT22I       781  ATGCAT               ATGCA^T
FbaI         1925  TGATCA               T^GATCA
Hin1I         592  GACGTC               G[GA]^CG[CT]C
HindIII       956  AAGCTT               A^AGCTT
MspA1I       2123  CAGCGG               C[AC]G^C[GT]G
NsiI          781  ATGCAT               ATGCA^T
PaeR7I        282  CTCGAG               C^TCGAG
PpuMI        2038  GGGACCC              [GA]G^G[AT]CC[CT]
SacI         1067  GAGCTC               GAGCT^C
SspBI        1310  TGTACA               T^GTACA
XhoI          282  CTCGAG               C^TCGAG
XmnI          198  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          912  GTATAC               GT^[AC][GT]AC
             1366  GTAGAC              
AflIII       1392  ACATGT               A^C[GA][CT]GT
             2654  ACGTGT              
AvaI          282  CTCGAG               C^[CT]CG[GA]G
             2168  CCCGAG              
BalI            2  TGGCCA               TGG^CCA
             2510  TGGCCA              
BamHI        1449  GGATCC               G^GATCC
             1644  GGATCC              
BanII         353  GGGCCC               G[GA]GC[CT]^C
             1067  GAGCTC              
BlpI         1019  GCTTAGC              GC^T[ACGT]AGC
             2312  GCTCAGC             
Bpu1102I     1019  GCTTAGC              GC^T[ACGT]AGC
             2312  GCTCAGC             
BsiYI         344  CCTTCTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1965  CCTGACTGTGG         
BslI          344  CCTTCTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1965  CCTGACTGTGG         
BsmAI        1013  GAGAC                GTCTC(1/5)
             1456  GAGAC               
BsmFI        2038  GGGAC                GGGAC(10/14)
              993  GTCCC               
BsoBI         282  CTCGAG               C^[CT]CG[GA]G
             2168  CCCGAG              
BsrDI        1241  GCAATG               GCAATG(2/0)
             2465  GCAATG              
BstAPI         24  GCAAAGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1024  GCAAATCCTGC         
CelII        1019  GCTTAGC              GC^T[ACGT]AGC
             2312  GCTCAGC             
EaeI            2  TGGCCA               [CT]^GGCC[GA]
             2510  TGGCCA              
HgaI          394  GACGC                GACGC(5/10)
              934  GACGC               
HincII        817  GTCAAC               GT[CT]^[GA]AC
             2211  GTCAAC              
HindII        817  GTCAAC               GT[CT]^[GA]AC
             2211  GTCAAC              
MluNI           2  TGGCCA               TGG^CCA
             2510  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
             2510  TGGCCA              
NdeI         1301  CATATG               CA^TATG
             2033  CATATG              
NspI          734  ACATGC               [GA]CATG^[CT]
             1392  ACATGT              
SmlI          282  CTCGAG               C^T[CT][GA]AG
              717  CTCAAG              
SspI         1105  AATATT               AAT^ATT
             1812  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
Aor51HI     ApoI        AspHI       AvaII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BsgI        BsiHKAI     Bsp1286I    
BspHI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       Eco47III    EcoO109I    EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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