Restriction map for pF1KB4058
 (Linear) (Six-base) MAPSORT of: KIBB4058.seq  Check: 3500  from: 1  to: 1611



KIBB4058.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     548    1611 
   Size:        548    1063
 
AflII C'TTAA_G

Cuts at:      0    1513    1611 
   Size:       1513      98
 
ApaI G_GGCC'C

Cuts at:      0     354    1611 
   Size:        354    1257
 
ApaLI G'TGCA_C

Cuts at:      0    1207    1611 
   Size:       1207     404
 
AvaI C'yCGr_G

Cuts at:      0     886    1611 
   Size:        886     725
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1429    1462    1611 
   Size:       1429      33     149

  Fragments arranged by size: 

                1429     149      33
 
BamHI G'GATC_C

Cuts at:      0     129     908    1067    1611 
   Size:        129     779     159     544

  Fragments arranged by size: 

                 779     544     159     129
 
BanII G_rGCy'C

Cuts at:      0     354     391     885    1611 
   Size:        354      37     494     726

  Fragments arranged by size: 

                 726     494     354      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1192    1480    1611 
   Size:       1192     288     131

  Fragments arranged by size: 

                1192     288     131
 
BbvCI CC'TCA_GC

Cuts at:      0      32    1611 
   Size:         32    1579
 
BclI T'GATC_A

Cuts at:      0    1098    1611 
   Size:       1098     513
 
BfrBI ATG'CAT

Cuts at:      0      84    1417    1611 
   Size:         84    1333     194

  Fragments arranged by size: 

                1333     194      84
 
Bme1580I G_kGCm'C

Cuts at:      0     354    1211    1611 
   Size:        354     857     400

  Fragments arranged by size: 

                 857     400     354
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     394    1611 
   Size:        394    1217
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     566     934    1248    1444    1611 
   Size:        425     141     368     314     196     167

  Fragments arranged by size: 

                 425     368     314     196     167     141
 
Bpu10I CC'TnA_GC

Cuts at:      0      32     636    1451    1611 
   Size:         32     604     815     160

  Fragments arranged by size: 

                 815     604     160      32
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1609    1611 
   Size:       1609       2
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1420    1611 
   Size:       1420     191
 
BsaAI yAC'GTr

Cuts at:      0    1206    1611 
   Size:       1206     405
 
BsaWI w'CCGG_w

Cuts at:      0    1499    1611 
   Size:       1499     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271    1066    1096    1396    1426    1611 
   Size:        241      30     795      30     300      30     185

  Fragments arranged by size: 

                 795     300     241     185      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     246     287     844     904    1126    1611 
   Size:        246      41     557      60     222     485

  Fragments arranged by size: 

                 557     485     246     222      60      41
 
BseYI C'CCAG_C

Cuts at:      0     874    1611 
   Size:        874     737
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     550     723    1611 
   Size:        550     173     888

  Fragments arranged by size: 

                 888     550     173
 
BsiHKAI G_wGCw'C

Cuts at:      0    1211    1611 
   Size:       1211     400
 
Bsp1286I G_dGCh'C

Cuts at:      0     354     391     885    1211    1611 
   Size:        354      37     494     326     400

  Fragments arranged by size: 

                 494     400     354     326      37
 
BsrBI CCG'CTC

Cuts at:      0      13    1611 
   Size:         13    1598
 
BsrDI GCAATG_nn'

Cuts at:      0    1139    1611 
   Size:       1139     472
 
BsrFI r'CCGG_y

Cuts at:      0      60    1611 
   Size:         60    1551
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     925    1611 
   Size:        925     686
 
BstYI r'GATC_y

Cuts at:      0     129     908    1067    1611 
   Size:        129     779     159     544

  Fragments arranged by size: 

                 779     544     159     129
 
Bsu36I CC'TnA_GG

Cuts at:      0    1333    1611 
   Size:       1333     278
 
BtgI C'CryG_G

Cuts at:      0     416    1611 
   Size:        416    1195
 
BtsI GCAGTG_nn'

Cuts at:      0      58    1611 
   Size:         58    1553
 
EaeI y'GGCC_r

Cuts at:      0     797    1611 
   Size:        797     814
 
EarI CTCTTCn'nnn_

Cuts at:      0     805     934    1057    1611 
   Size:        805     129     123     554

  Fragments arranged by size: 

                 805     554     129     123
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     179    1611 
   Size:        179    1432
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     548     566     934    1248    1444    1611 
   Size:        425     123      18     368     314     196     167

  Fragments arranged by size: 

                 425     368     314     196     167     123      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     274    1611 
   Size:        274    1337
 
EcoO109I rG'GnC_Cy

Cuts at:      0     350     351     422     561     961    1368    1395    1611 
   Size:        350       1      71     139     400     407      27     216

  Fragments arranged by size: 

                 407     400     350     216     139      71      27       1
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     699    1611 
   Size:        699     912
 
HaeII r_GCGC'y

Cuts at:      0     756    1611 
   Size:        756     855
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     619     651    1166    1198    1270    1302 
   Size:        241      32     346      32     515      32      72      32

Cuts at:   1302    1611 
   Size:        309

  Fragments arranged by size: 

                 515     346     309     241      72      32      32      32
                  32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1400    1611 
   Size:       1400     211
 
MscI TGG'CCA

Cuts at:      0     799    1611 
   Size:        799     812
 
MslI CAynn'nnrTG

Cuts at:      0     805    1306    1582    1611 
   Size:        805     501     276      29

  Fragments arranged by size: 

                 805     501     276      29
 
MspA1I CmG'CkG

Cuts at:      0      36     139     227     662     759     767     840    1027 
   Size:         36     103      88     435      97       8      73     187

Cuts at:   1027    1611 
   Size:        584

  Fragments arranged by size: 

                 584     435     187     103      97      88      73      36
                   8
 
NcoI C'CATG_G

Cuts at:      0     416    1611 
   Size:        416    1195
 
NsiI A_TGCA'T

Cuts at:      0      86    1419    1611 
   Size:         86    1333     192

  Fragments arranged by size: 

                1333     192      86
 
NspI r_CATG'y

Cuts at:      0      43    1611 
   Size:         43    1568
 
PasI CC'CwG_GG

Cuts at:      0     276     505     592    1611 
   Size:        276     229      87    1019

  Fragments arranged by size: 

                1019     276     229      87
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     337    1605    1611 
   Size:        337    1268       6

  Fragments arranged by size: 

                1268     337       6
 
PfoI T'CCnGG_A

Cuts at:      0     911    1611 
   Size:        911     700
 
PmlI CAC'GTG

Cuts at:      0    1206    1611 
   Size:       1206     405
 
PpuMI rG'GwC_Cy

Cuts at:      0     422     961    1395    1611 
   Size:        422     539     434     216

  Fragments arranged by size: 

                 539     434     422     216
 
PspOMI G'GGCC_C

Cuts at:      0     350    1611 
   Size:        350    1261
 
PvuII CAG'CTG

Cuts at:      0     662    1027    1611 
   Size:        662     365     584

  Fragments arranged by size: 

                 662     584     365
 
SanDI GG'GwC_CC

Cuts at:      0     422    1611 
   Size:        422    1189
 
ScaI AGT'ACT

Cuts at:      0     775    1611 
   Size:        775     836
 
SmlI C'TyrA_G

Cuts at:      0    1513    1588    1611 
   Size:       1513      75      23

  Fragments arranged by size: 

                1513      75      23
 
SphI G_CATG'C

Cuts at:      0      43    1611 
   Size:         43    1568
 
StyI C'CwwG_G

Cuts at:      0     336     416    1364    1611 
   Size:        336      80     948     247

  Fragments arranged by size: 

                 948     336     247      80
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1244    1611 
   Size:       1244     367
 
TatI w'GTAC_w

Cuts at:      0     773    1611 
   Size:        773     838
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1066    1098    1611 
   Size:       1066      32     513

  Fragments arranged by size: 

                1066     513      32
 
XbaI T'CTAG_A

Cuts at:      0    1521    1611 
   Size:       1521      90
 

 Enzymes that do cut:

     AcuI    AflII     ApaI    ApaLI     AvaI     BaeI    BamHI    BanII
     BbsI    BbvCI     BclI    BfrBI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BsrBI    BsrDI    BsrFI   BstAPI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I     MmeI     MscI     MslI   MspA1I     NcoI
     NsiI     NspI     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI
    PvuII    SanDI     ScaI     SmlI     SphI     StyI    TaqII     TatI
     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApoI     AscI     AseI
    AsiSI    AvrII     BanI     BbeI     BcgI    BciVI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI    BsaBI    BsaHI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
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