Restriction map for
pF1KB4041
(Linear) (Six-base) MAPSORT of: KIBB4041.seq Check: 2167 from: 1 to: 1389
KIBB4041.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AfeI AGC'GCT
Cuts at: 0 280 1389
Size: 280 1109
AgeI A'CCGG_T
Cuts at: 0 964 1389
Size: 964 425
AleI CACnn'nnGTG
Cuts at: 0 881 965 1389
Size: 881 84 424
Fragments arranged by size:
881 424 84
ApaLI G'TGCA_C
Cuts at: 0 1068 1389
Size: 1068 321
ApoI r'AATT_y
Cuts at: 0 210 384 536 788 872 1208 1389
Size: 210 174 152 252 84 336 181
Fragments arranged by size:
336 252 210 181 174 152 84
AvaI C'yCGr_G
Cuts at: 0 926 1389
Size: 926 463
BamHI G'GATC_C
Cuts at: 0 1376 1389
Size: 1376 13
BanI G'GyrC_C
Cuts at: 0 346 1389
Size: 346 1043
BanII G_rGCy'C
Cuts at: 0 598 689 1389
Size: 598 91 700
Fragments arranged by size:
700 598 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 754 1389
Size: 754 635
BciVI GTATCCnnnnn_n'
Cuts at: 0 1034 1389
Size: 1034 355
BclI T'GATC_A
Cuts at: 0 308 1389
Size: 308 1081
Bme1580I G_kGCm'C
Cuts at: 0 1072 1389
Size: 1072 317
BmgBI CAC'GTC
Cuts at: 0 99 1389
Size: 99 1290
BmrI ACTGGGnnnn_n'
Cuts at: 0 102 554 722 1389
Size: 102 452 168 667
Fragments arranged by size:
667 452 168 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 267 1389
Size: 127 140 1122
Fragments arranged by size:
1122 140 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1389
Size: 582 807
BsaI GGTCTCn'nnnn_
Cuts at: 0 132 1389
Size: 132 1257
BsaBI GATnn'nnATC
Cuts at: 0 310 1389
Size: 310 1079
BsaWI w'CCGG_w
Cuts at: 0 964 1389
Size: 964 425
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1389
Size: 67 1322
BseYI C'CCAG_C
Cuts at: 0 1010 1389
Size: 1010 379
BsiHKAI G_wGCw'C
Cuts at: 0 689 1072 1389
Size: 689 383 317
Fragments arranged by size:
689 383 317
BsmI GAATG_Cn'
Cuts at: 0 736 1389
Size: 736 653
Bsp1286I G_dGCh'C
Cuts at: 0 598 689 1072 1389
Size: 598 91 383 317
Fragments arranged by size:
598 383 317 91
BspHI T'CATG_A
Cuts at: 0 186 1389
Size: 186 1203
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 339 1389
Size: 339 1050
BsrFI r'CCGG_y
Cuts at: 0 964 1389
Size: 964 425
BsrGI T'GTAC_A
Cuts at: 0 1237 1389
Size: 1237 152
BssSI C'ACGA_G
Cuts at: 0 1033 1117 1192 1389
Size: 1033 84 75 197
Fragments arranged by size:
1033 197 84 75
BstYI r'GATC_y
Cuts at: 0 956 1376 1389
Size: 956 420 13
Fragments arranged by size:
956 420 13
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 723 758 1389
Size: 723 35 631
Fragments arranged by size:
723 631 35
EaeI y'GGCC_r
Cuts at: 0 237 1266 1389
Size: 237 1029 123
Fragments arranged by size:
1029 237 123
EarI CTCTTCn'nnn_
Cuts at: 0 1246 1389
Size: 1246 143
EcoICRI GAG'CTC
Cuts at: 0 687 1389
Size: 687 702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 267 1389
Size: 127 140 1122
Fragments arranged by size:
1122 140 127
EcoO109I rG'GnC_Cy
Cuts at: 0 84 1389
Size: 84 1305
EcoRI G'AATT_C
Cuts at: 0 210 384 788 872 1208 1389
Size: 210 174 404 84 336 181
Fragments arranged by size:
404 336 210 181 174 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 280 516 1389
Size: 280 236 873
Fragments arranged by size:
873 280 236
HaeII r_GCGC'y
Cuts at: 0 282 1389
Size: 282 1107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 1175 1207 1245 1277 1389
Size: 127 32 1016 32 38 32 112
Fragments arranged by size:
1016 127 112 38 32 32 32
HincII GTy'rAC
Cuts at: 0 442 1389
Size: 442 947
MfeI C'AATT_G
Cuts at: 0 1092 1389
Size: 1092 297
MslI CAynn'nnrTG
Cuts at: 0 461 629 881 965 1389
Size: 461 168 252 84 424
Fragments arranged by size:
461 424 252 168 84
NdeI CA'TA_TG
Cuts at: 0 150 1389
Size: 150 1239
NspI r_CATG'y
Cuts at: 0 1343 1389
Size: 1343 46
PasI CC'CwG_GG
Cuts at: 0 6 1389
Size: 6 1383
PflMI CCAn_nnn'nTGG
Cuts at: 0 12 1389
Size: 12 1377
PpuMI rG'GwC_Cy
Cuts at: 0 84 1389
Size: 84 1305
PspXI vC'TCGA_Gb
Cuts at: 0 926 1389
Size: 926 463
SacI G_AGCT'C
Cuts at: 0 689 1389
Size: 689 700
SapI GCTCTTCn'nnn_
Cuts at: 0 1246 1389
Size: 1246 143
SfcI C'TryA_G
Cuts at: 0 114 170 1389
Size: 114 56 1219
Fragments arranged by size:
1219 114 56
SgrAI Cr'CCGG_yG
Cuts at: 0 964 1389
Size: 964 425
SmlI C'TyrA_G
Cuts at: 0 597 926 1389
Size: 597 329 463
Fragments arranged by size:
597 463 329
StuI AGG'CCT
Cuts at: 0 415 499 667 919 1003 1087 1389
Size: 415 84 168 252 84 84 302
Fragments arranged by size:
415 302 252 168 84 84 84
StyI C'CwwG_G
Cuts at: 0 234 1389
Size: 234 1155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1223 1389
Size: 1223 166
TatI w'GTAC_w
Cuts at: 0 89 1237 1389
Size: 89 1148 152
Fragments arranged by size:
1148 152 89
XhoI C'TCGA_G
Cuts at: 0 926 1389
Size: 926 463
XmnI GAAnn'nnTTC
Cuts at: 0 499 1087 1171 1389
Size: 499 588 84 218
Fragments arranged by size:
588 499 218 84
Enzymes that do cut:
AfeI AgeI AleI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BciVI BclI Bme1580I BmgBI BmrI BpmI
BpuEI BsaI BsaBI BsaWI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstYI CspCI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII MfeI MslI NdeI NspI PasI PflMI
PpuMI PspXI SacI SapI SfcI SgrAI SmlI StuI
StyI TaqII TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BfrBI BglI BglII
BlpI BmtI BplI Bpu10I BsaAI BsaHI BsaXI BsgI
BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BssHII BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MluI
MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XmaI ZraI