Restriction map for pF1KB4020
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AseI        
AsnI        Asp700I     Asp718I     AspEI       BamHI       
BanI        BbeI        BbrPI       BbvCI       BciVI       
BclI        BfrI        BglII       BsaAI       BsaBI       
BsaHI       BseAI       BsgI        BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrDI       
BsrGI       Bst1107I    BstBI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MaeII       MamI        MluI        
MroI        MslI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
TfiI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          130  GTCTAC               GT^[AC][GT]AC
AgeI         1442  ACCGGT               A^CCGGT
ApaI          746  GGGCCC               GGGCC^C
AscI           20  GGCGCGCC             GG^CGCGCC
AspI            6  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         525  CCTAGG               C^CTAGG
BbsI          792  GAAGAC               GAAGAC(2/6)
BcgI          887  GCATCCGCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI           80  CCCGG                CC^[GC]GG
BlnI          525  CCTAGG               C^CTAGG
BpuAI         792  GAAGAC               GAAGAC(2/6)
BsaWI        1442  ACCGGT               [AT]^CCGG[AT]
BsiEI         138  CGACCG               CG[GA][CT]^CG
BsiWI        1579  CGTACG               C^GTACG
BspHI         663  TCATGA               T^CATGA
BssSI         865  CACGAG               CACGAG(-5/-1)
BstYI        1115  AGATCC               [GA]^GATC[CT]
DraIII       1226  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1915  GACAATCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         525  CCTAGGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI         1241  GCGTC                GACGC(5/10)
MfeI         1395  CAATTG               C^AATTG
MflI         1115  AGATCC               [GA]^GATC[CT]
MseI          338  TTAA                 T^TAA
MunI         1395  CAATTG               C^AATTG
NciI           80  CCCGG                CC^[GC]GG
NcoI         1388  CCATGG               C^CATGG
PflFI           6  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1383  CCAGTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1442  ACCGGT               A^CCGGT
RcaI          663  TCATGA               T^CATGA
SapI         1841  GCTCTTC              GCTCTTC(1/4)
SbfI          102  CCTGCAGG             CCTGCA^GG
SmlI          514  CTTGAG               C^T[CT][GA]AG
Sse8387I      102  CCTGCAGG             CCTGCA^GG
Tru9I         338  TTAA                 T^TAA
Tsp509I      1396  AATT                 ^AATT
Tth111I         6  GACCCCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1383  CCAGTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1115  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AlwI         1116  GATCC                GGATC(4/5)
             1426  GATCC               
AvaI          253  CTCGGG               C^[CT]CG[GA]G
             1716  CTCGGG              
AviII        1361  TGCGCA               TGC^GCA
             1619  TGCGCA              
BalI          506  TGGCCA               TGG^CCA
             1613  TGGCCA              
BfaI          526  CTAG                 C^TAG
             1307  CTAG                
BlpI          315  GCTGAGC              GC^T[ACGT]AGC
              915  GCTCAGC             
Bpu1102I      315  GCTGAGC              GC^T[ACGT]AGC
              915  GCTCAGC             
BsoBI         253  CTCGGG               C^[CT]CG[GA]G
             1716  CTCGGG              
BstAPI        486  GCATGATCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              735  GCAGGAACTGC         
BstEII        373  GGTGACC              G^GT[ACGT]ACC
             1923  GGTCACC             
BstPI         373  GGTGACC              G^GT[ACGT]ACC
             1923  GGTCACC             
CelII         315  GCTGAGC              GC^T[ACGT]AGC
              915  GCTCAGC             
EarI         1842  CTCTTC               CTCTTC(1/4)
              199  GAAGAG              
EcoO65I       373  GGTGACC              G^GT[ACGT]ACC
             1923  GGTCACC             
FspI         1361  TGCGCA               TGC^GCA
             1619  TGCGCA              
HaeII         656  AGCGCC               [GA]GCGC^[CT]
             1409  AGCGCC              
HindIII       511  AAGCTT               A^AGCTT
              955  AAGCTT              
Ksp632I      1842  CTCTTC               CTCTTC(1/4)
              199  GAAGAG              
MaeI          526  CTAG                 C^TAG
             1307  CTAG                
MluNI         506  TGGCCA               TGG^CCA
             1613  TGGCCA              
MscI          506  TGGCCA               TGG^CCA
             1613  TGGCCA              
NaeI          716  GCCGGC               GCC^GGC
             1001  GCCGGC              
NgoMIV        716  GCCGGC               G^CCGGC
             1001  GCCGGC              
SacI          109  GAGCTC               GAGCT^C
              250  GAGCTC              
XcmI         1221  CCAGACACAGGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1616  CCATGCGCAAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BanII       BbvI        BglI        
BmyI        BpmI        BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SexAI       SfaNI       
SfcI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     
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