Restriction map for pF1KB3912
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvrII       BalI        BamHI       
BanII       BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BsaAI       BsaBI       BsaI        
BsiEI       BsiHKAI     BsiWI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
HaeIII      HincII      HindII      HindIII     HinfI       
HpaI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MflI        MluI        
MluNI       MscI        MspA1I      MunI        MvnI        
NaeI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp45I      
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII        290  TCCGGA               T^CCGGA
AciI          161  GCGG                 CCGC(-3/-1)
AcyI          255  GGCGCC               G[GA]^CG[CT]C
AfaI          406  GTAC                 GT^AC
AvaII         145  GGACC                G^G[AT]CC
AviII         325  TGCGCA               TGC^GCA
BanI          255  GGCGCC               G^G[CT][GA]CC
BbeI          255  GGCGCC               GGCGC^C
BbsI           17  GTCTTC               GAAGAC(2/6)
BlpI          166  GCTGAGC              GC^T[ACGT]AGC
BmyI          365  GTGCCC               G[AGT]GC[ACT]^C
Bpu1102I      166  GCTGAGC              GC^T[ACGT]AGC
BpuAI          17  GTCTTC               GAAGAC(2/6)
BsaHI         255  GGCGCC               G[GA]^CG[CT]C
BsaJI         137  CCACGG               C^C[ACGT][ACGT]GG
BseAI         290  TCCGGA               T^CCGGA
BseRI          84  CTCCTC               GAGGAG(10/8)
BsgI          434  GTGCAG               GTGCAG(16/14)
BsmBI         151  GAGACG               CGTCTC(1/5)
Bsp1286I      365  GTGCCC               G[AGT]GC[ACT]^C
BspEI         290  TCCGGA               T^CCGGA
BssKI         102  CCTGG                ^CC[ACGT]GG
BstNI         102  CCTGG                CC^[AT]GG
CelII         166  GCTGAGC              GC^T[ACGT]AGC
Cfr13I        145  GGACC                G^G[ACGT]CC
DpnII         301  GATC                 ^GATC
DsaI          137  CCACGG               C^C[GA][CT]GG
EcoNI         259  CCTCAGACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        102  CCTGG                ^CC[AT]GG
FauI          161  GCGGG                CCCGC(4/6)
FspI          325  TGCGCA               TGC^GCA
HgaI          153  GACGC                GACGC(5/10)
Hin1I         255  GGCGCC               G[GA]^CG[CT]C
KasI          255  GGCGCC               G^GCGCC
MboI          301  GATC                 ^GATC
MroI          290  TCCGGA               T^CCGGA
MseI           35  TTAA                 T^TAA
MslI          378  CACCGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          102  CCTGG                CC^[AT]GG
NarI          255  GGCGCC               GG^CGCC
NdeII         301  GATC                 ^GATC
NspI          383  ACATGC               [GA]CATG^[CT]
RsaI          406  GTAC                 GT^AC
SapI          181  GCTCTTC              GCTCTTC(1/4)
Sau3AI        301  GATC                 ^GATC
Sau96I        145  GGACC                G^G[ACGT]CC
ScrFI         102  CCTGG                CC^[ACGT]GG
SfcI          450  CTACAG               C^T[GA][CT]AG
SfoI          255  GGCGCC               GGC^GCC
SmlI          334  CTCAAG               C^T[CT][GA]AG
Tru9I          35  TTAA                 T^TAA
Tsp509I        29  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BbvI           41  GCTGC                GCAGC(8/12)
              284  GCTGC               
BpmI          240  CTCCAG               CTGGAG(16/14)
              313  CTGGAG              
BsaWI         147  ACCGGA               [AT]^CCGG[AT]
              290  TCCGGA              
BsiYI         242  CCAGCAGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              259  CCTCAGACAGG         
BslI          242  CCAGCAGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              259  CCTCAGACAGG         
BsmAI         151  GAGAC                GTCTC(1/5)
              200  GAGAC               
EarI           94  CTCTTC               CTCTTC(1/4)
              182  CTCTTC              
Fnu4HI         41  GCTGC                GC^[ACGT]GC
              284  GCTGC               
HapII         148  CCGG                 C^CGG
              291  CCGG                
HpaII         148  CCGG                 C^CGG
              291  CCGG                
ItaI           41  GCTGC                GC^[ACGT]GC
              284  GCTGC               
Ksp632I        94  CTCTTC               CTCTTC(1/4)
              182  CTCTTC              
MspI          148  CCGG                 C^CGG
              291  CCGG                
NlaIII         78  CATG                 CATG^
              384  CATG                
NlaIV         238  GGCTCC               GG[ACGT]^[ACGT]CC
              255  GGCGCC              
TseI           41  GCTGC                G^C[AT]GC
              284  GCTGC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BstF5I      Cac8I       CfoI        DdeI        
FokI        HaeII       HhaI        HinP1I      HphI        
MboII       MnlI        MwoI        SfaNI       
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