Restriction map for pF1KB3864
##################################
# NO CUT ENZYMES (   92 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvrII       BbrPI       
BcgI        BclI        BfaI        BfrI        BlnI        
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BspDI       BspEI       BspLU11I    Bst1107I    
BstBI       BstZ17I     ClaI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoRI       
EcoRV       FbaI        FseI        HindIII     HpaI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PvuI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tru9I       Tsp509I     Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

Acc65I       1805  GGTACC               G^GTACC
Aor51HI      2630  AGCGCT               AGC^GCT
Asp718I      1805  GGTACC               G^GTACC
AviII        2459  TGCGCA               TGC^GCA
BamHI        3041  GGATCC               G^GATCC
BciVI        3101  GTATCC               GTATCC(6/5)
BglII        2204  AGATCT               A^GATCT
BlpI         2005  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2005  GCTCAGC              GC^T[ACGT]AGC
BsaAI         983  TACGTA               [CT]AC^GT[GA]
BsaWI        2794  TCCGGT               [AT]^CCGG[AT]
Bsp1407I     3074  TGTACA               T^GTACA
BspHI        1482  TCATGA               T^CATGA
BsrGI        3074  TGTACA               T^GTACA
BssHII        259  GCGCGC               G^CGCGC
BstEII       1219  GGTCACC              G^GT[ACGT]ACC
BstPI        1219  GGTCACC              G^GT[ACGT]ACC
CelII        2005  GCTCAGC              GC^T[ACGT]AGC
Eco47III     2630  AGCGCT               AGC^GCT
EcoNI          42  CCTACCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1219  GGTCACC              G^GT[ACGT]ACC
EcoT22I      3193  ATGCAT               ATGCA^T
FspI         2459  TGCGCA               TGC^GCA
HincII       2907  GTTGAC               GT[CT]^[GA]AC
HindII       2907  GTTGAC               GT[CT]^[GA]AC
KpnI         1805  GGTACC               GGTAC^C
NsiI         3193  ATGCAT               ATGCA^T
PshAI        1653  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1482  TCATGA               T^CATGA
SnaBI         983  TACGTA               TAC^GTA
SspBI        3074  TGTACA               T^GTACA
StuI          528  AGGCCT               AGG^CCT


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AocI          470  CCTCAGG              CC^T[ACGT]AGG
             2302  CCTGAGG             
BbeI           31  GGCGCC               GGCGC^C
             1799  GGCGCC              
BsaI         2242  GAGACC               GGTCTC(1/5)
             2119  GGTCTC              
BsgI         1840  CTGCAC               GTGCAG(16/14)
             3412  CTGCAC              
BsrDI        1452  CATTGC               GCAATG(2/0)
             3307  CATTGC              
Bsu36I        470  CCTCAGG              CC^T[ACGT]AGG
             2302  CCTGAGG             
CpoI         1382  CGGTCCG              CG^G[AT]CCG
             2879  CGGTCCG             
DraIII        928  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1673  CACGGGGTG           
Eco81I        470  CCTCAGG              CC^T[ACGT]AGG
             2302  CCTGAGG             
KasI           31  GGCGCC               G^GCGCC
             1799  GGCGCC              
NaeI          740  GCCGGC               GCC^GGC
             3138  GCCGGC              
NarI           31  GGCGCC               GG^CGCC
             1799  GGCGCC              
NcoI         1104  CCATGG               C^CATGG
             1130  CCATGG              
NgoMIV        740  GCCGGC               G^CCGGC
             3138  GCCGGC              
NspI         1202  ACATGC               [GA]CATG^[CT]
             3416  ACATGC              
PvuII        3106  CAGCTG               CAG^CTG
             3214  CAGCTG              
RsrII        1382  CGGTCCG              CG^G[AT]CCG
             2879  CGGTCCG             
SacI         1056  GAGCTC               GAGCT^C
             2003  GAGCTC              
SapI         1866  GCTCTTC              GCTCTTC(1/4)
             3324  GCTCTTC             
SfoI           31  GGCGCC               GGC^GCC
             1799  GGCGCC              
SmlI         1366  CTTGAG               C^T[CT][GA]AG
             2785  CTCAAG              
TfiI          439  GAATC                G^A[AT]TC
             1083  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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