Restriction map for pF1KB3864
 (Linear) (Six-base) MAPSORT of: KIBB3864.seq  Check: 6480  from: 1  to: 3426



KIBB3864.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     819    3214    3426 
   Size:        819    2395     212

  Fragments arranged by size: 

                2395     819     212
 
Acc65I G'GTAC_C

Cuts at:      0    1805    3426 
   Size:       1805    1621
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1018    1258    1280    1760    3426 
   Size:       1018     240      22     480    1666

  Fragments arranged by size: 

                1666    1018     480     240      22
 
AfeI AGC'GCT

Cuts at:      0    2632    3426 
   Size:       2632     794
 
AleI CACnn'nnGTG

Cuts at:      0    1279    1972    2983    3426 
   Size:       1279     693    1011     443

  Fragments arranged by size: 

                1279    1011     693     443
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2374    3247    3426 
   Size:       2374     873     179

  Fragments arranged by size: 

                2374     873     179
 
AlwNI CAG_nnn'CTG

Cuts at:      0     454     861    2013    2088    2589    2698    3120    3351 
   Size:        454     407    1152      75     501     109     422     231

Cuts at:   3351    3426 
   Size:         75

  Fragments arranged by size: 

                1152     501     454     422     407     231     109      75
                  75
 
ApaI G_GGCC'C

Cuts at:      0     577     711    1412    2313    2377    3426 
   Size:        577     134     701     901      64    1049

  Fragments arranged by size: 

                1049     901     701     577     134      64
 
AvaI C'yCGr_G

Cuts at:      0      25     175     704     710    2385    2431    3426 
   Size:         25     150     529       6    1675      46     995

  Fragments arranged by size: 

                1675     995     529     150      46      25       6
 
BamHI G'GATC_C

Cuts at:      0    3041    3426 
   Size:       3041     385
 
BanI G'GyrC_C

Cuts at:      0      31      57     831    1122    1799    1805    3294    3426 
   Size:         31      26     774     291     677       6    1489     132

  Fragments arranged by size: 

                1489     774     677     291     132      31      26       6
            
 
BanII G_rGCy'C

Cuts at:      0      74     464     577     595     711     754    1060    1412 
   Size:         74     390     113      18     116      43     306     352

Cuts at:   1412    1726    1768    2007    2082    2313    2377    2609    2794 
   Size:        314      42     239      75     231      64     232     185

Cuts at:   2794    2840    3426 
   Size:         46     586

  Fragments arranged by size: 

                 586     390     352     314     306     239     232     231
                 185     116     113      75      74      64      46      43
                  42      18
 
BbeI G_GCGC'C

Cuts at:      0      35    1803    3426 
   Size:         35    1768    1623

  Fragments arranged by size: 

                1768    1623      35
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1933    2127    3187    3426 
   Size:       1933     194    1060     239

  Fragments arranged by size: 

                1933    1060     239     194
 
BbvCI CC'TCA_GC

Cuts at:      0     625    1268    1888    3426 
   Size:        625     643     620    1538

  Fragments arranged by size: 

                1538     643     625     620
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3112    3426 
   Size:       3112     314
 
BfrBI ATG'CAT

Cuts at:      0    3195    3426 
   Size:       3195     231
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1498    2152    2604    3005    3303    3426 
   Size:       1498     654     452     401     298     123

  Fragments arranged by size: 

                1498     654     452     401     298     123
 
BglII A'GATC_T

Cuts at:      0    2204    3426 
   Size:       2204    1222
 
BlpI GC'TnA_GC

Cuts at:      0    2006    3426 
   Size:       2006    1420
 
Bme1580I G_kGCm'C

Cuts at:      0      62     577     711     790     801     836    1412    2313 
   Size:         62     515     134      79      11      35     576     901

Cuts at:   2313    2358    2377    2664    3426 
   Size:         45      19     287     762

  Fragments arranged by size: 

                 901     762     576     515     287     134      79      62
                  45      35      19      11
 
BmgBI CAC'GTC

Cuts at:      0      90    3089    3185    3426 
   Size:         90    2999      96     241

  Fragments arranged by size: 

                2999     241      96      90
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1814    1842    3426 
   Size:       1814      28    1584

  Fragments arranged by size: 

                1814    1584      28
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     360    3426 
   Size:        360    3066
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416     588     768     922    1044    1267    1375    2110 
   Size:        416     172     180     154     122     223     108     735

Cuts at:   2110    2335    2359    3426 
   Size:        225      24    1067

  Fragments arranged by size: 

                1067     735     416     225     223     180     172     154
                 122     108      24
 
Bpu10I CC'TnA_GC

Cuts at:      0     625    1268    1627    1888    3155    3426 
   Size:        625     643     359     261    1267     271

  Fragments arranged by size: 

                1267     643     625     359     271     261
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1387    2770    3426 
   Size:       1387    1383     656

  Fragments arranged by size: 

                1387    1383     656
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2125    2236    3426 
   Size:       2125     111    1190

  Fragments arranged by size: 

                2125    1190     111
 
BsaAI yAC'GTr

Cuts at:      0     985    3426 
   Size:        985    2441
 
BsaHI Gr'CG_yC

Cuts at:      0      32    1181    1800    1982    3426 
   Size:         32    1149     619     182    1444

  Fragments arranged by size: 

                1444    1149     619     182      32
 
BsaWI w'CCGG_w

Cuts at:      0    2794    3426 
   Size:       2794     632
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     333     363     383     413     769     799     954     984 
   Size:        333      30      20      30     356      30     155      30

Cuts at:    984    3426 
   Size:       2442

  Fragments arranged by size: 

                2442     356     333     155      30      30      30      30
                  20
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82     109     112     341     397     427     436    2701 
   Size:         82      27       3     229      56      30       9    2265

Cuts at:   2701    2806    3426 
   Size:        105     620

  Fragments arranged by size: 

                2265     620     229     105      82      56      30      27
                   9       3
 
BseYI C'CCAG_C

Cuts at:      0     184     596     688     697     727     861     973    1099 
   Size:        184     412      92       9      30     134     112     126

Cuts at:   1099    1404    1411    1917    1998    2380    3113    3216    3426 
   Size:        305       7     506      81     382     733     103     210

  Fragments arranged by size: 

                 733     506     412     382     305     210     184     134
                 126     112     103      92      81      30       9       7
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1825    3397    3426 
   Size:       1825    1572      29

  Fragments arranged by size: 

                1825    1572      29
 
BsiHKAI G_wGCw'C

Cuts at:      0     619    1060    1868    1979    2007    2279    2990    3059 
   Size:        619     441     808     111      28     272     711      69

Cuts at:   3059    3426 
   Size:        367

  Fragments arranged by size: 

                 808     711     619     441     367     272     111      69
                  28
 
Bsp1286I G_dGCh'C

Cuts at:      0      62      74     464     577     595     619     711     754 
   Size:         62      12     390     113      18      24      92      43

Cuts at:    754     790     801     836    1060    1412    1726    1768    1868 
   Size:         36      11      35     224     352     314      42     100

Cuts at:   1868    1979    2007    2082    2279    2313    2358    2377    2609 
   Size:        111      28      75     197      34      45      19     232

Cuts at:   2609    2664    2794    2840    2990    3059    3426 
   Size:         55     130      46     150      69     367

  Fragments arranged by size: 

                 390     367     352     314     232     224     197     150
                 130     113     111     100      92      75      69      62
                  55      46      45      43      42      36      35      34
                  28      24      19      18      12      11
 
BspHI T'CATG_A

Cuts at:      0    1482    3426 
   Size:       1482    1944
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     125     779     819    1598    1835    2819    3214    3426 
   Size:        125     654      40     779     237     984     395     212

  Fragments arranged by size: 

                 984     779     654     395     237     212     125      40
            
 
BsrBI CCG'CTC

Cuts at:      0     204     252    2885    3426 
   Size:        204      48    2633     541

  Fragments arranged by size: 

                2633     541     204      48
 
BsrDI GCAATG_nn'

Cuts at:      0    1451    3306    3426 
   Size:       1451    1855     120

  Fragments arranged by size: 

                1855    1451     120
 
BsrFI r'CCGG_y

Cuts at:      0     221     740    1508    1802    3138    3426 
   Size:        221     519     768     294    1336     288

  Fragments arranged by size: 

                1336     768     519     294     288     221
 
BsrGI T'GTAC_A

Cuts at:      0    3074    3426 
   Size:       3074     352
 
BssHII G'CGCG_C

Cuts at:      0     259    3426 
   Size:        259    3167
 
BssSI C'ACGA_G

Cuts at:      0    1435    2634    3001    3426 
   Size:       1435    1199     367     425

  Fragments arranged by size: 

                1435    1199     425     367
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     614     831    2283    3426 
   Size:        614     217    1452    1143

  Fragments arranged by size: 

                1452    1143     614     217
 
BstEII G'GTnAC_C

Cuts at:      0    1219    3426 
   Size:       1219    2207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     651    1207    3003    3021    3426 
   Size:        651     556    1796      18     405

  Fragments arranged by size: 

                1796     651     556     405      18
 
BstYI r'GATC_y

Cuts at:      0    1253    2204    2951    3041    3065    3426 
   Size:       1253     951     747      90      24     361

  Fragments arranged by size: 

                1253     951     747     361      90      24
 
Bsu36I CC'TnA_GG

Cuts at:      0     471    2303    3426 
   Size:        471    1832    1123

  Fragments arranged by size: 

                1832    1123     471
 
BtgI C'CryG_G

Cuts at:      0    1104    1130    1274    1601    2677    2876    3251    3426 
   Size:       1104      26     144     327    1076     199     375     175

  Fragments arranged by size: 

                1104    1076     375     327     199     175     144      26
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2913    2976    3210    3426 
   Size:       2913      63     234     216

  Fragments arranged by size: 

                2913     234     216      63
 
BtsI GCAGTG_nn'

Cuts at:      0     801    2281    2358    2407    2664    3229    3426 
   Size:        801    1480      77      49     257     565     197

  Fragments arranged by size: 

                1480     801     565     257     197      77      49
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     795     830    1011    1046    3426 
   Size:        795      35     181      35    2380

  Fragments arranged by size: 

                2380     795     181      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     933    1678    3426 
   Size:        933     745    1748

  Fragments arranged by size: 

                1748     933     745
 
EaeI y'GGCC_r

Cuts at:      0       2     758    1165    1856    2103    2153    3390    3426 
   Size:          2     756     407     691     247      50    1237      36

  Fragments arranged by size: 

                1237     756     691     407     247      50      36       2
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     404     413     422     883    1399    1873    1993    3331 
   Size:        404       9       9     461     516     474     120    1338

Cuts at:   3331    3426 
   Size:         95

  Fragments arranged by size: 

                1338     516     474     461     404     120      95       9
                   9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     862    3204    3426 
   Size:        862    2342     222

  Fragments arranged by size: 

                2342     862     222
 
EcoICRI GAG'CTC

Cuts at:      0    1058    2005    3426 
   Size:       1058     947    1421

  Fragments arranged by size: 

                1421    1058     947
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416     588     768     922    1018    1044    1258    1267 
   Size:        416     172     180     154      96      26     214       9

Cuts at:   1267    1280    1375    1760    2110    2335    2359    3426 
   Size:         13      95     385     350     225      24    1067

  Fragments arranged by size: 

                1067     416     385     350     225     214     180     172
                 154      96      95      26      24      13       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      46    3426 
   Size:         46    3380
 
EcoO109I rG'GnC_Cy

Cuts at:      0     144     171     365     476    1371    1408    2085    2309 
   Size:        144      27     194     111     895      37     677     224

Cuts at:   2309    2310    2321    2373    2374    2435    3426 
   Size:          1      11      52       1      61     991

  Fragments arranged by size: 

                 991     895     677     224     194     144     111      61
                  52      37      27      11       1       1
 
FspI TGC'GCA

Cuts at:      0    2461    3426 
   Size:       2461     965
 
HaeII r_GCGC'y

Cuts at:      0      35    1449    1803    2634    2755    3352    3426 
   Size:         35    1414     354     831     121     597      74

  Fragments arranged by size: 

                1414     831     597     354     121      74      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     954     986    2617    2649    3426 
   Size:        954      32    1631      32     777

  Fragments arranged by size: 

                1631     954     777      32      32
 
HincII GTy'rAC

Cuts at:      0    2909    3426 
   Size:       2909     517
 
KasI G'GCGC_C

Cuts at:      0      31    1799    3426 
   Size:         31    1768    1627

  Fragments arranged by size: 

                1768    1627      31
 
KpnI G_GTAC'C

Cuts at:      0    1809    3426 
   Size:       1809    1617
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3019    3426 
   Size:       3019     407
 
MscI TGG'CCA

Cuts at:      0       4    1167    3392    3426 
   Size:          4    1163    2225      34

  Fragments arranged by size: 

                2225    1163      34       4
 
MslI CAynn'nnrTG

Cuts at:      0    1279    1462    1972    2425    2539    2900    2983    3019 
   Size:       1279     183     510     453     114     361      83      36

Cuts at:   3019    3133    3421    3426 
   Size:        114     288       5

  Fragments arranged by size: 

                1279     510     453     361     288     183     114     114
                  83      36       5
 
MspA1I CmG'CkG

Cuts at:      0    1861    1972    2013    2040    2698    2973    3108    3216 
   Size:       1861     111      41      27     658     275     135     108

Cuts at:   3216    3426 
   Size:        210

  Fragments arranged by size: 

                1861     658     275     210     135     111     108      41
                  27
 
NaeI GCC'GGC

Cuts at:      0     742    3140    3426 
   Size:        742    2398     286

  Fragments arranged by size: 

                2398     742     286
 
NarI GG'CG_CC

Cuts at:      0      32    1800    3426 
   Size:         32    1768    1626

  Fragments arranged by size: 

                1768    1626      32
 
NcoI C'CATG_G

Cuts at:      0    1104    1130    3426 
   Size:       1104      26    2296

  Fragments arranged by size: 

                2296    1104      26
 
NgoMIV G'CCGG_C

Cuts at:      0     740    3138    3426 
   Size:        740    2398     288

  Fragments arranged by size: 

                2398     740     288
 
NsiI A_TGCA'T

Cuts at:      0    3197    3426 
   Size:       3197     229
 
NspI r_CATG'y

Cuts at:      0    1206    3420    3426 
   Size:       1206    2214       6

  Fragments arranged by size: 

                2214    1206       6
 
PasI CC'CwG_GG

Cuts at:      0     535     801    1693    2652    2829    3426 
   Size:        535     266     892     959     177     597

  Fragments arranged by size: 

                 959     892     597     535     266     177
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     861    1854    2088    3426 
   Size:        861     993     234    1338

  Fragments arranged by size: 

                1338     993     861     234
 
PfoI T'CCnGG_A

Cuts at:      0     377     946    1352    1541    2954    3329    3426 
   Size:        377     569     406     189    1413     375      97

  Fragments arranged by size: 

                1413     569     406     377     375     189      97
 
PpuMI rG'GwC_Cy

Cuts at:      0     144     171     365     476    1371    2085    2321    2435 
   Size:        144      27     194     111     895     714     236     114

Cuts at:   2435    3426 
   Size:        991

  Fragments arranged by size: 

                 991     895     714     236     194     144     114     111
                  27
 
PshAI GACnn'nnGTC

Cuts at:      0    1657    3426 
   Size:       1657    1769
 
PspOMI G'GGCC_C

Cuts at:      0     573     707    1408    2309    2373    3426 
   Size:        573     134     701     901      64    1053

  Fragments arranged by size: 

                1053     901     701     573     134      64
 
PstI C_TGCA'G

Cuts at:      0     873    1320    3347    3426 
   Size:        873     447    2027      79

  Fragments arranged by size: 

                2027     873     447      79
 
PvuII CAG'CTG

Cuts at:      0    3108    3216    3426 
   Size:       3108     108     210

  Fragments arranged by size: 

                3108     210     108
 
RsrII CG'GwC_CG

Cuts at:      0    1383    2880    3426 
   Size:       1383    1497     546

  Fragments arranged by size: 

                1497    1383     546
 
SacI G_AGCT'C

Cuts at:      0    1060    2007    3426 
   Size:       1060     947    1419

  Fragments arranged by size: 

                1419    1060     947
 
SanDI GG'GwC_CC

Cuts at:      0     171     365     476    2321    3426 
   Size:        171     194     111    1845    1105

  Fragments arranged by size: 

                1845    1105     194     171     111
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1873    3331    3426 
   Size:       1873    1458      95

  Fragments arranged by size: 

                1873    1458      95
 
SfcI C'TryA_G

Cuts at:      0     195     869    1316    2600    3343    3426 
   Size:        195     674     447    1284     743      83

  Fragments arranged by size: 

                1284     743     674     447     195      83
 
SfoI GGC'GCC

Cuts at:      0      33    1801    3426 
   Size:         33    1768    1625

  Fragments arranged by size: 

                1768    1625      33
 
SmaI CCC'GGG

Cuts at:      0      27     706     712    3426 
   Size:         27     679       6    2714

  Fragments arranged by size: 

                2714     679      27       6
 
SmlI C'TyrA_G

Cuts at:      0    1366    2785    3426 
   Size:       1366    1419     641

  Fragments arranged by size: 

                1419    1366     641
 
SnaBI TAC'GTA

Cuts at:      0     985    3426 
   Size:        985    2441
 
SrfI GCCC'GGGC

Cuts at:      0     712    3426 
   Size:        712    2714
 
StuI AGG'CCT

Cuts at:      0     530    3426 
   Size:        530    2896
 
StyI C'CwwG_G

Cuts at:      0      62     122     667    1104    1130    1184    3173    3426 
   Size:         62      60     545     437      26      54    1989     253

  Fragments arranged by size: 

                1989     545     437     253      62      60      54      26
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     256    1106    1945    2212    2294    2908    3228    3426 
   Size:        256     850     839     267      82     614     320     198

  Fragments arranged by size: 

                 850     839     614     320     267     256     198      82
            
 
TatI w'GTAC_w

Cuts at:      0    1347    3074    3401    3426 
   Size:       1347    1727     327      25

  Fragments arranged by size: 

                1727    1347     327      25
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     383     415     769     801    3426 
   Size:        383      32     354      32    2625

  Fragments arranged by size: 

                2625     383     354      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1111    1160    2535    2541    3426 
   Size:       1111      49    1375       6     885

  Fragments arranged by size: 

                1375    1111     885      49       6
 
XmaI C'CCGG_G

Cuts at:      0      25     704     710    3426 
   Size:         25     679       6    2716

  Fragments arranged by size: 

                2716     679      25       6
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     AleI     AlfI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI    PflMI
     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII    RsrII     SacI
    SanDI     SapI     SfcI     SfoI     SmaI     SmlI    SnaBI     SrfI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BcgI
     BclI     BmtI    BsaBI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BstBI  BstZ17I     ClaI     DraI     DrdI     EagI    EcoRI    EcoRV
     FalI     FseI    FspAI  HindIII     HpaI     MfeI     MluI     NdeI
     NheI     NotI     NruI     PacI     PciI     PmeI     PmlI     PpiI
     PsiI    PspXI     PsrI     PvuI    SacII     SalI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SpeI     SphI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com