Restriction map for pF1KB3827
##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
ApaI        AscI        AseI        AsnI        Asp718I     
AspI        AviII       BbvCI       BciVI       BfrI        
BsiWI       BspDI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        CpoI        
EcoO65I     FseI        FspI        HpaI        KpnI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RsrII       SalI        SbfI        SfiI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI         1336  GTCTAC               GT^[AC][GT]AC
AccIII        458  TCCGGA               T^CCGGA
AhdI         2441  GACACAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1381  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       255  AGCGCT               AGC^GCT
Asp700I      1467  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        2441  GACACAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         157  CCTAGG               C^CTAGG
BamHI        3120  GGATCC               G^GATCC
BcgI         2726  CGACGGCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          158  CTAG                 C^TAG
BlnI          157  CCTAGG               C^CTAGG
BlpI           39  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I       39  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1255  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         458  TCCGGA               T^CCGGA
BsmBI         324  GAGACG               CGTCTC(1/5)
Bsp1407I     1057  TGTACA               T^GTACA
BspEI         458  TCCGGA               T^CCGGA
BspLU11I     2652  ACATGT               A^CATGT
BsrGI        1057  TGTACA               T^GTACA
BssHII         44  GCGCGC               G^CGCGC
Bsu36I       1381  CCTGAGG              CC^T[ACGT]AGG
CelII          39  GCTGAGC              GC^T[ACGT]AGC
DraI         3364  TTTAAA               TTT^AAA
DraIII       1328  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2632  GACAGCGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          315  CGGCCG               C^GGCCG
Eam1105I     2441  GACACAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         315  CGGCCG               C^GGCCG
Eco47III      255  AGCGCT               AGC^GCT
Eco52I        315  CGGCCG               C^GGCCG
Eco81I       1381  CCTGAGG              CC^T[ACGT]AGG
EcoRI        2923  GAATTC               G^AATTC
EcoRV        3165  GATATC               GAT^ATC
KspI         2389  CCGCGG               CCGC^GG
MaeI          158  CTAG                 C^TAG
MamI         1255  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          458  TCCGGA               T^CCGGA
PflMI         522  CCAAGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         1510  CTGCAG               CTGCA^G
SacI          612  GAGCTC               GAGCT^C
SacII        2389  CCGCGG               CCGC^GG
SapI         1750  GAAGAGC              GCTCTTC(1/4)
ScaI         2296  AGTACT               AGT^ACT
SexAI        3411  ACCTGGT              A^CC[AT]GGT
SmlI         1000  CTTGAG               C^T[CT][GA]AG
SspBI        1057  TGTACA               T^GTACA
Van91I        522  CCAAGCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2499  CCAAGTGGACTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1467  GAAGACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   39 enzymes) #
###################################

AcsI          901  GAATTT               [GA]^AATT[CT]
             2923  GAATTC              
Alw44I       1546  GTGCAC               G^TGCAC
             2677  GTGCAC              
ApaLI        1546  GTGCAC               G^TGCAC
             2677  GTGCAC              
ApoI          901  GAATTT               [GA]^AATT[CT]
             2923  GAATTC              
BalI          543  TGGCCA               TGG^CCA
             2217  TGGCCA              
BbeI           13  GGCGCC               GGCGC^C
             1515  GGCGCC              
BbrPI        1328  CACGTG               CAC^GTG
             2674  CACGTG              
BbsI          850  GAAGAC               GAAGAC(2/6)
             1467  GAAGAC              
BclI          767  TGATCA               T^GATCA
             2801  TGATCA              
BglI         1379  GCCCTGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2747  GCCAAGTGGGC         
BglII        1739  AGATCT               A^GATCT
             1852  AGATCT              
BpuAI         850  GAAGAC               GAAGAC(2/6)
             1467  GAAGAC              
BsaWI         458  TCCGGA               [AT]^CCGG[AT]
             2927  TCCGGT              
BsiEI         315  CGGCCG               CG[GA][CT]^CG
             3003  CGGTCG              
BspHI        1394  TCATGA               T^CATGA
             1934  TCATGA              
BspMI        2607  GCAGGT               ACCTGC(4/8)
             2865  GCAGGT              
BsrDI        1620  CATTGC               GCAATG(2/0)
             2213  GCAATG              
BstAPI       1548  GCACTGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3490  GCACAGTATGC         
EcoT22I      1190  ATGCAT               ATGCA^T
             1237  ATGCAT              
FbaI          767  TGATCA               T^GATCA
             2801  TGATCA              
HgaI           89  GCGTC                GACGC(5/10)
              222  GCGTC               
HincII        627  GTCAAC               GT[CT]^[GA]AC
             3144  GTCAAC              
HindII        627  GTCAAC               GT[CT]^[GA]AC
             3144  GTCAAC              
HindIII       825  AAGCTT               A^AGCTT
             1207  AAGCTT              
KasI           13  GGCGCC               G^GCGCC
             1515  GGCGCC              
MluNI         543  TGGCCA               TGG^CCA
             2217  TGGCCA              
MscI          543  TGGCCA               TGG^CCA
             2217  TGGCCA              
MseI         3256  TTAA                 T^TAA
             3365  TTAA                
NarI           13  GGCGCC               GG^CGCC
             1515  GGCGCC              
NsiI         1190  ATGCAT               ATGCA^T
             1237  ATGCAT              
PmaCI        1328  CACGTG               CAC^GTG
             2674  CACGTG              
PmlI         1328  CACGTG               CAC^GTG
             2674  CACGTG              
PpuMI         434  GGGACCC              [GA]G^G[AT]CC[CT]
             1538  GGGACCC             
PshAI        2614  GACAACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2998  GACATCGGTC          
RcaI         1394  TCATGA               T^CATGA
             1934  TCATGA              
SfcI         1510  CTGCAG               C^T[GA][CT]AG
             2031  CTACAG              
SfoI           13  GGCGCC               GGC^GCC
             1515  GGCGCC              
SgrAI          15  CGCCGGCG             C[GA]^CCGG[CT]G
               54  CGCCGGCG            
Tru9I        3256  TTAA                 T^TAA
             3365  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       AspHI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmaI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmaCI       XmaI        
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