Restriction map for
pF1KB3827
(Circular) (Six-base) MAPSORT of: pF1KB3827.seq Check: 5794 from: 1 to: 6617
pF1KB3827 6617 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2706 2706
Size: 6617
AccI GT'mk_AC
Cuts at: 1445 3655 1445
Size: 2210 4407
Acc65I G'GTAC_C
Cuts at: 3633 3633
Size: 6617
AclI AA'CG_TT
Cuts at: 6457 6457
Size: 6617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1714 1805 2046 2919 2930 3346 4442 4874 5621
Size: 91 241 873 11 416 1096 432 747
Cuts at: 5621 5889 1714
Size: 268 2442
Fragments arranged by size:
2442 1096 873 747 432 416 268 241
91 11
AfeI AGC'GCT
Cuts at: 365 365
Size: 6617
AflIII A'CryG_T
Cuts at: 1280 1409 1435 2676 2760 3969 5073 6050 1280
Size: 129 26 1241 84 1209 1104 977 1847
Fragments arranged by size:
1847 1241 1209 1104 977 129 84 26
AgeI A'CCGG_T
Cuts at: 5005 5005
Size: 6617
AhdI GACnn_n'nnGTC
Cuts at: 2554 2554
Size: 6617
AleI CACnn'nnGTG
Cuts at: 2875 2875
Size: 6617
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 6585
AlwNI CAG_nnn'CTG
Cuts at: 1607 1798 2334 3922 5489 1607
Size: 191 536 1588 1567 2735
Fragments arranged by size:
2735 1588 1567 536 191
ApaLI G'TGCA_C
Cuts at: 1654 2785 5387 1654
Size: 1131 2602 2884
Fragments arranged by size:
2884 2602 1131
ApoI r'AATT_y
Cuts at: 1009 3031 3621 5970 6226 1009
Size: 2022 590 2349 256 1400
Fragments arranged by size:
2349 2022 1400 590 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6617
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6617
AvaI C'yCGr_G
Cuts at: 192 352 383 491 533 3637 192
Size: 160 31 108 42 3104 3172
Fragments arranged by size:
3172 3104 160 108 42 31
AvrII C'CTAG_G
Cuts at: 265 265
Size: 6617
BamHI G'GATC_C
Cuts at: 3228 3642 3228
Size: 414 6203
BanI G'GyrC_C
Cuts at: 121 1623 2863 3633 4047 4295 4330 121
Size: 1502 1240 770 414 248 35 2408
Fragments arranged by size:
2408 1502 1240 770 414 248 35
BanII G_rGCy'C
Cuts at: 176 303 384 490 724 3631 4661 176
Size: 127 81 106 234 2907 1030 2132
Fragments arranged by size:
2907 2132 1030 234 127 106 81
BbeI G_GCGC'C
Cuts at: 125 1627 4051 4299 125
Size: 1502 2424 248 2443
Fragments arranged by size:
2443 2424 1502 248
BbsI GAAGACnn'nnnn_
Cuts at: 965 1582 965
Size: 617 6000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2823 2857 2823
Size: 34 6583
BciVI GTATCCnnnnn_n'
Cuts at: 4515 5276 6617 4515
Size: 761 1341 4515
Fragments arranged by size:
4515 1341 761
BclI T'GATC_A
Cuts at: 875 2909 4137 875
Size: 2034 1228 3355
Fragments arranged by size:
3355 2034 1228
BfrBI ATG'CAT
Cuts at: 1300 1347 1300
Size: 47 6570
BglI GCCn_nnn'nGGC
Cuts at: 1493 2861 1493
Size: 1368 5249
BglII A'GATC_T
Cuts at: 1847 1960 4132 6169 1847
Size: 113 2172 2037 2295
Fragments arranged by size:
2295 2172 2037 113
BlpI GC'TnA_GC
Cuts at: 148 3729 148
Size: 3581 3036
Bme1580I G_kGCm'C
Cuts at: 391 1658 2485 2789 2866 3600 4242 4335 5391
Size: 1267 827 304 77 734 642 93 1056
Cuts at: 5391 391
Size: 1617
Fragments arranged by size:
1617 1267 1056 827 734 642 304 93
77
BmgBI CAC'GTC
Cuts at: 2679 2679
Size: 6617
BmrI ACTGGGnnnn_n'
Cuts at: 401 2417 3829 3910 4245 6389 401
Size: 2016 1412 81 335 2144 629
Fragments arranged by size:
2144 2016 1412 629 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4984 4984
Size: 6617
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1860 2045 3257 3286 394
Size: 1466 185 1212 29 3725
Fragments arranged by size:
3725 1466 1212 185 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 3793 4672 5164 5462 1129
Size: 2664 879 492 298 2284
Fragments arranged by size:
2664 2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1704 2982 3044 6321 1704
Size: 1278 62 3277 2000
Fragments arranged by size:
3277 2000 1278 62
BsaAI yAC'GTr
Cuts at: 1281 1412 1438 2784 3970 1281
Size: 131 26 1346 1186 3928
Fragments arranged by size:
3928 1346 1186 131 26
BsaBI GATnn'nnATC
Cuts at: 6 1367 4131 6168 6
Size: 1361 2764 2037 455
Fragments arranged by size:
2764 2037 1361 455
BsaHI Gr'CG_yC
Cuts at: 122 197 1624 4048 4296 4998 6494 122
Size: 75 1427 2424 248 702 1496 245
Fragments arranged by size:
2424 1496 1427 702 248 245 75
BsaWI w'CCGG_w
Cuts at: 566 3035 3806 4327 5005 5279 5426 5837 566
Size: 2469 771 521 678 274 147 411 1346
Fragments arranged by size:
2469 1346 771 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1883 1913 3326 3356 1883
Size: 30 1413 30 5144
Fragments arranged by size:
5144 1413 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 148 444 589 3114 148
Size: 296 145 2525 3651
Fragments arranged by size:
3651 2525 296 145
BseYI C'CCAG_C
Cuts at: 235 2851 2983 3592 4042 4800 5377 5873 235
Size: 2616 132 609 450 758 577 496 979
Fragments arranged by size:
2616 979 758 609 577 496 450 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 398 2140 2259 2734 2992 398
Size: 1742 119 475 258 4023
Fragments arranged by size:
4023 1742 475 258 119
BsiEI CG_ry'CG
Cuts at: 104 426 3114 4159 4205 5413 104
Size: 322 2688 1045 46 1208 1308
Fragments arranged by size:
2688 1308 1208 1045 322 46
BsiHKAI G_wGCw'C
Cuts at: 724 1658 1865 2783 2789 3182 3631 4409 4599
Size: 934 207 918 6 393 449 778 190
Cuts at: 4599 5391 724
Size: 792 1950
Fragments arranged by size:
1950 934 918 792 778 449 393 207
190 6
BsmI GAATG_Cn'
Cuts at: 1160 1302 2994 1160
Size: 142 1692 4783
Fragments arranged by size:
4783 1692 142
BsmBI CGTCTCn'nnnn_
Cuts at: 426 426
Size: 6617
Bsp1286I G_dGCh'C
Cuts at: 176 303 384 391 490 724 1658 1865 2485
Size: 127 81 7 99 234 934 207 620
Cuts at: 2485 2783 2789 2866 3182 3600 3631 4242 4335
Size: 298 6 77 316 418 31 611 93
Cuts at: 4335 4409 4599 4661 5391 176
Size: 74 190 62 730 1402
Fragments arranged by size:
1402 934 730 620 611 418 316 298
234 207 190 127 99 93 81 77
74 62 31 7 6
BspEI T'CCGG_A
Cuts at: 566 5837 566
Size: 5271 1346
BspHI T'CATG_A
Cuts at: 1502 2042 5793 1502
Size: 540 3751 2326
Fragments arranged by size:
3751 2326 540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2706 2964 3667 3881 4183 4564 2706
Size: 258 703 214 302 381 4759
Fragments arranged by size:
4759 703 381 302 258 214
BsrBI CCG'CTC
Cuts at: 50 378 440 445 540 2072 2220 4909 4963
Size: 328 62 5 95 1532 148 2689 54
Cuts at: 4963 6447 6612 50
Size: 1484 165 55
Fragments arranged by size:
2689 1532 1484 328 165 148 95 62
55 54 5
BsrDI GCAATG_nn'
Cuts at: 1727 2328 4529 1727
Size: 601 2201 3815
Fragments arranged by size:
3815 2201 601
BsrFI r'CCGG_y
Cuts at: 124 163 911 2337 4615 4796 5005 124
Size: 39 748 1426 2278 181 209 1736
Fragments arranged by size:
2278 1736 1426 748 209 181 39
BsrGI T'GTAC_A
Cuts at: 1165 1165
Size: 6617
BssHII G'CGCG_C
Cuts at: 152 152
Size: 6617
BssSI C'ACGA_G
Cuts at: 4888 5246 4888
Size: 358 6259
BstAPI GCAn_nnn'nTGC
Cuts at: 1662 3604 3994 1662
Size: 1942 390 4285
Fragments arranged by size:
4285 1942 390
BstBI TT'CG_AA
Cuts at: 4978 4978
Size: 6617
BstXI CCAn_nnnn'nTGG
Cuts at: 649 1122 2859 649
Size: 473 1737 4407
Fragments arranged by size:
4407 1737 473
BstYI r'GATC_y
Cuts at: 1 1635 1847 1960 3228 3406 3586 3642 4109
Size: 1634 212 113 1268 178 180 56 467
Cuts at: 4109 4132 4467 4713 5725 5811 5823 6169 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1634 1268 1012 467 449 346 335 246
212 180 178 113 86 56 23 12
Bsu36I CC'TnA_GG
Cuts at: 1490 1490
Size: 6617
BtgI C'CryG_G
Cuts at: 107 401 635 2497 2657 107
Size: 294 234 1862 160 4067
Fragments arranged by size:
4067 1862 294 234 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2636 3000 4567 4748 4911 2636
Size: 364 1567 181 163 4342
Fragments arranged by size:
4342 1567 364 181 163
BtsI GCAGTG_nn'
Cuts at: 1656 2462 2846 3197 3458 3860 1656
Size: 806 384 351 261 402 4413
Fragments arranged by size:
4413 806 402 384 351 261
DraI TTT'AAA
Cuts at: 3474 3616 3474
Size: 142 6475
DraIII CAC_nnn'GTG
Cuts at: 1441 1441
Size: 6617
DrdI GACnn_nn'nnGTC
Cuts at: 2746 4323 5181 2746
Size: 1577 858 4182
Fragments arranged by size:
4182 1577 858
EaeI y'GGCC_r
Cuts at: 289 423 651 1267 2325 4202 4376 4767 4794
Size: 134 228 616 1058 1877 174 391 27
Cuts at: 4794 6139 289
Size: 1345 767
Fragments arranged by size:
1877 1345 1058 767 616 391 228 174
134 27
EagI C'GGCC_G
Cuts at: 423 4202 423
Size: 3779 2838
EarI CTCTTCn'nnn_
Cuts at: 1824 1853 2024 2126 2573 2801 3047 4640 4850
Size: 29 171 102 447 228 246 1593 210
Cuts at: 4850 1824
Size: 3591
Fragments arranged by size:
3591 1593 447 246 228 210 171 102
29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3554 4825 5135 5281 6427 3554
Size: 1271 310 146 1146 3744
Fragments arranged by size:
3744 1271 1146 310 146
EcoICRI GAG'CTC
Cuts at: 722 3629 722
Size: 2907 3710
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 1714 1805 1860 2045 2046 2919 2930 3257
Size: 1320 91 55 185 1 873 11 327
Cuts at: 3257 3286 3346 4442 4874 5621 5889 394
Size: 29 60 1096 432 747 268 1122
Fragments arranged by size:
1320 1122 1096 873 747 432 327 268
185 91 60 55 29 11 1
EcoNI CCTnn'n_nnAGG
Cuts at: 1193 1290 1516 1193
Size: 97 226 6294
Fragments arranged by size:
6294 226 97
EcoO109I rG'GnC_Cy
Cuts at: 543 1486 1647 3756 543
Size: 943 161 2109 3404
Fragments arranged by size:
3404 2109 943 161
EcoRI G'AATT_C
Cuts at: 3031 3621 3031
Size: 590 6027
EcoRV GAT'ATC
Cuts at: 3275 3275
Size: 6617
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4636 4636
Size: 6617
FspI TGC'GCA
Cuts at: 4398 4398
Size: 6617
HaeII r_GCGC'y
Cuts at: 125 367 1627 2747 4051 4299 5321 6300 125
Size: 242 1260 1120 1304 248 1022 979 442
Fragments arranged by size:
1304 1260 1120 1022 979 442 248 242
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1198 1230 2089 2121 2540 2572 3259 3291 3326
Size: 32 859 32 419 32 687 32 35
Cuts at: 3326 3332 3358 3364 4459 4489 4491 4521 1198
Size: 6 26 6 1095 30 2 30 3294
Fragments arranged by size:
3294 1095 859 687 419 35 32 32
32 32 30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 737 3254 3656 737
Size: 2517 402 3698
Fragments arranged by size:
3698 2517 402
HindIII A'AGCT_T
Cuts at: 933 1315 933
Size: 382 6235
KasI G'GCGC_C
Cuts at: 121 1623 4047 4295 121
Size: 1502 2424 248 2443
Fragments arranged by size:
2443 2424 1502 248
KpnI G_GTAC'C
Cuts at: 3637 3637
Size: 6617
MfeI C'AATT_G
Cuts at: 3608 3608
Size: 6617
MluI A'CGCG_T
Cuts at: 6050 6050
Size: 6617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 463 3418 5288 5472 463
Size: 2955 1870 184 1608
Fragments arranged by size:
2955 1870 1608 184
MscI TGG'CCA
Cuts at: 653 2327 4378 653
Size: 1674 2051 2892
Fragments arranged by size:
2892 2051 1674
MslI CAynn'nnrTG
Cuts at: 1759 1807 2047 2321 2563 2608 2875 2968 3169
Size: 48 240 274 242 45 267 93 201
Cuts at: 3169 3418 4606 4733 1759
Size: 249 1188 127 3643
Fragments arranged by size:
3643 1188 274 267 249 242 240 201
127 93 48 45
MspA1I CmG'CkG
Cuts at: 1251 1384 2085 2154 2499 2539 2595 2983 3235
Size: 133 701 69 345 40 56 388 252
Cuts at: 3235 3370 3728 4042 4402 5415 5660 1251
Size: 135 358 314 360 1013 245 2208
Fragments arranged by size:
2208 1013 701 388 360 358 345 314
252 245 135 133 69 56 40
NaeI GCC'GGC
Cuts at: 126 165 2339 4798 126
Size: 39 2174 2459 1945
Fragments arranged by size:
2459 2174 1945 39
NarI GG'CG_CC
Cuts at: 122 1624 4048 4296 122
Size: 1502 2424 248 2443
Fragments arranged by size:
2443 2424 1502 248
NcoI C'CATG_G
Cuts at: 107 401 635 2657 107
Size: 294 234 2022 4067
Fragments arranged by size:
4067 2022 294 234
NgoMIV G'CCGG_C
Cuts at: 124 163 2337 4796 124
Size: 39 2174 2459 1945
Fragments arranged by size:
2459 2174 1945 39
NsiI A_TGCA'T
Cuts at: 1302 1349 1302
Size: 47 6570
NspI r_CATG'y
Cuts at: 1347 1351 1812 2177 2764 3670 4170 5077 1347
Size: 4 461 365 587 906 500 907 2887
Fragments arranged by size:
2887 907 906 587 500 461 365 4
PasI CC'CwG_GG
Cuts at: 2485 2485
Size: 6617
PciI A'CATG_T
Cuts at: 2760 5073 2760
Size: 2313 4304
PflMI CCAn_nnn'nTGG
Cuts at: 636 636
Size: 6617
PmeI GTTT'AAAC
Cuts at: 3616 3616
Size: 6617
PmlI CAC'GTG
Cuts at: 1438 2784 1438
Size: 1346 5271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5756 5788 5756
Size: 32 6585
PpuMI rG'GwC_Cy
Cuts at: 543 1647 543
Size: 1104 5513
PshAI GACnn'nnGTC
Cuts at: 2726 3110 2726
Size: 384 6233
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1764 1796 1764
Size: 32 6585
PstI C_TGCA'G
Cuts at: 1622 3664 4349 1622
Size: 2042 685 3890
Fragments arranged by size:
3890 2042 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6617
PvuII CAG'CTG
Cuts at: 1251 2085 2154 2983 3235 4042 4402 1251
Size: 834 69 829 252 807 360 3466
Fragments arranged by size:
3466 834 829 807 360 252 69
RsrII CG'GwC_CG
Cuts at: 4812 4812
Size: 6617
SacI G_AGCT'C
Cuts at: 724 3631 724
Size: 2907 3710
SacII CC_GC'GG
Cuts at: 2500 2500
Size: 6617
SalI G'TCGA_C
Cuts at: 3654 3654
Size: 6617
SanDI GG'GwC_CC
Cuts at: 543 1647 543
Size: 1104 5513
SapI GCTCTTCn'nnn_
Cuts at: 1853 4640 4850 1853
Size: 2787 210 3620
Fragments arranged by size:
3620 2787 210
SbfI CC_TGCA'GG
Cuts at: 3664 3664
Size: 6617
ScaI AGT'ACT
Cuts at: 2406 6134 2406
Size: 3728 2889
SexAI A'CCwGG_T
Cuts at: 3519 3519
Size: 6617
SfcI C'TryA_G
Cuts at: 33 1618 2139 3660 4345 5338 5529 33
Size: 1585 521 1521 685 993 191 1121
Fragments arranged by size:
1585 1521 1121 993 685 521 191
SfoI GGC'GCC
Cuts at: 123 1625 4049 4297 123
Size: 1502 2424 248 2443
Fragments arranged by size:
2443 2424 1502 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6617
SgrAI Cr'CCGG_yG
Cuts at: 124 163 124
Size: 39 6578
SmaI CCC'GGG
Cuts at: 194 354 493 3639 194
Size: 160 139 3146 3172
Fragments arranged by size:
3172 3146 160 139
SmlI C'TyrA_G
Cuts at: 1108 3772 4687 5179 5441 1108
Size: 2664 915 492 262 2284
Fragments arranged by size:
2664 2284 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6617
SphI G_CATG'C
Cuts at: 3670 4170 3670
Size: 500 6117
StyI C'CwwG_G
Cuts at: 107 265 401 635 1337 1460 2146 2657 2774
Size: 158 136 234 702 123 686 511 117
Cuts at: 2774 2915 3751 4105 107
Size: 141 836 354 2619
Fragments arranged by size:
2619 836 702 686 511 354 234 158
141 136 123 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 374 2005 3100 4200 4333 4792 5001 374
Size: 1631 1095 1100 133 459 209 1990
Fragments arranged by size:
1990 1631 1100 1095 459 209 133
TatI w'GTAC_w
Cuts at: 1165 2404 2459 2753 3312 6132 1165
Size: 1239 55 294 559 2820 1650
Fragments arranged by size:
2820 1650 1239 559 294 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1974 2006 3135 3167 6134 6166 1974
Size: 32 1129 32 2967 32 2425
Fragments arranged by size:
2967 2425 1129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4414 4414
Size: 6617
XbaI T'CTAG_A
Cuts at: 3648 3648
Size: 6617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 4112 2614
Size: 1498 5119
XmaI C'CCGG_G
Cuts at: 192 352 491 3637 192
Size: 160 139 3146 3172
Fragments arranged by size:
3172 3146 160 139
XmnI GAAnn'nnTTC
Cuts at: 1579 1579
Size: 6617
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI ApaI AscI BaeI BbvCI BmtI
Bpu10I BsiWI BstEII BstZ17I ClaI CspCI FseI FspAI
HpaI NdeI NheI NotI NruI PacI PfoI PsiI
PspOMI PspXI SfiI SnaBI SrfI SspI StuI SwaI
XhoI ZraI