Restriction map for pF1KB3827
 (Linear) (Six-base) MAPSORT of: KIBB3827.seq  Check: 1337  from: 1  to: 3504



KIBB3827.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2598    3504 
   Size:       2598     906
 
AccI GT'mk_AC

Cuts at:      0    1337    3504 
   Size:       1337    2167
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1606    1697    1938    2811    2822    3238    3504 
   Size:       1606      91     241     873      11     416     266

  Fragments arranged by size: 

                1606     873     416     266     241      91      11
 
AfeI AGC'GCT

Cuts at:      0     257    3504 
   Size:        257    3247
 
AflIII A'CryG_T

Cuts at:      0    1172    1301    1327    2568    2652    3504 
   Size:       1172     129      26    1241      84     852

  Fragments arranged by size: 

                1241    1172     852     129      84      26
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2446    3504 
   Size:       2446    1058
 
AleI CACnn'nnGTG

Cuts at:      0    2767    3504 
   Size:       2767     737
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3089    3121    3504 
   Size:       3089      32     383

  Fragments arranged by size: 

                3089     383      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1499    1690    2226    3504 
   Size:       1499     191     536    1278

  Fragments arranged by size: 

                1499    1278     536     191
 
ApaLI G'TGCA_C

Cuts at:      0    1546    2677    3504 
   Size:       1546    1131     827

  Fragments arranged by size: 

                1546    1131     827
 
ApoI r'AATT_y

Cuts at:      0     901    2923    3504 
   Size:        901    2022     581

  Fragments arranged by size: 

                2022     901     581
 
AvaI C'yCGr_G

Cuts at:      0      84     244     275     383     425    3504 
   Size:         84     160      31     108      42    3079

  Fragments arranged by size: 

                3079     160     108      84      42      31
 
AvrII C'CTAG_G

Cuts at:      0     157    3504 
   Size:        157    3347
 
BamHI G'GATC_C

Cuts at:      0    3120    3504 
   Size:       3120     384
 
BanI G'GyrC_C

Cuts at:      0      13    1515    2755    3504 
   Size:         13    1502    1240     749

  Fragments arranged by size: 

                1502    1240     749      13
 
BanII G_rGCy'C

Cuts at:      0      68     195     276     382     616    3504 
   Size:         68     127      81     106     234    2888

  Fragments arranged by size: 

                2888     234     127     106      81      68
 
BbeI G_GCGC'C

Cuts at:      0      17    1519    3504 
   Size:         17    1502    1985

  Fragments arranged by size: 

                1985    1502      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     857    1474    3504 
   Size:        857     617    2030

  Fragments arranged by size: 

                2030     857     617
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2715    2749    3504 
   Size:       2715      34     755

  Fragments arranged by size: 

                2715     755      34
 
BclI T'GATC_A

Cuts at:      0     767    2801    3504 
   Size:        767    2034     703

  Fragments arranged by size: 

                2034     767     703
 
BfrBI ATG'CAT

Cuts at:      0    1192    1239    3504 
   Size:       1192      47    2265

  Fragments arranged by size: 

                2265    1192      47
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1385    2753    3504 
   Size:       1385    1368     751

  Fragments arranged by size: 

                1385    1368     751
 
BglII A'GATC_T

Cuts at:      0    1739    1852    3504 
   Size:       1739     113    1652

  Fragments arranged by size: 

                1739    1652     113
 
BlpI GC'TnA_GC

Cuts at:      0      40    3504 
   Size:         40    3464
 
Bme1580I G_kGCm'C

Cuts at:      0     283    1550    2377    2681    2758    3492    3504 
   Size:        283    1267     827     304      77     734      12

  Fragments arranged by size: 

                1267     827     734     304     283      77      12
 
BmgBI CAC'GTC

Cuts at:      0    2571    3504 
   Size:       2571     933
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     293    2309    3504 
   Size:        293    2016    1195

  Fragments arranged by size: 

                2016    1195     293
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286    1752    1937    3149    3178    3504 
   Size:        286    1466     185    1212      29     326

  Fragments arranged by size: 

                1466    1212     326     286     185      29
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1021    3504 
   Size:       1021    2483
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1596    2874    2936    3504 
   Size:       1596    1278      62     568

  Fragments arranged by size: 

                1596    1278     568      62
 
BsaAI yAC'GTr

Cuts at:      0    1173    1304    1330    2676    3504 
   Size:       1173     131      26    1346     828

  Fragments arranged by size: 

                1346    1173     828     131      26
 
BsaBI GATnn'nnATC

Cuts at:      0    1259    3504 
   Size:       1259    2245
 
BsaHI Gr'CG_yC

Cuts at:      0      14      89    1516    3504 
   Size:         14      75    1427    1988

  Fragments arranged by size: 

                1988    1427      75      14
 
BsaWI w'CCGG_w

Cuts at:      0     458    2927    3504 
   Size:        458    2469     577

  Fragments arranged by size: 

                2469     577     458
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1775    1805    3218    3248    3504 
   Size:       1775      30    1413      30     256

  Fragments arranged by size: 

                1775    1413     256      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     336     481    3006    3504 
   Size:         40     296     145    2525     498

  Fragments arranged by size: 

                2525     498     296     145      40
 
BseYI C'CCAG_C

Cuts at:      0     127    2743    2875    3484    3504 
   Size:        127    2616     132     609      20

  Fragments arranged by size: 

                2616     609     132     127      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     290    2032    2151    2626    2884    3504 
   Size:        290    1742     119     475     258     620

  Fragments arranged by size: 

                1742     620     475     290     258     119
 
BsiEI CG_ry'CG

Cuts at:      0     318    3006    3504 
   Size:        318    2688     498

  Fragments arranged by size: 

                2688     498     318
 
BsiHKAI G_wGCw'C

Cuts at:      0     616    1550    1757    2675    2681    3074    3504 
   Size:        616     934     207     918       6     393     430

  Fragments arranged by size: 

                 934     918     616     430     393     207       6
 
BsmI GAATG_Cn'

Cuts at:      0    1052    1194    2886    3504 
   Size:       1052     142    1692     618

  Fragments arranged by size: 

                1692    1052     618     142
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     318    3504 
   Size:        318    3186
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     195     276     283     382     616    1550    1757 
   Size:         68     127      81       7      99     234     934     207

Cuts at:   1757    2377    2675    2681    2758    3074    3492    3504 
   Size:        620     298       6      77     316     418      12

  Fragments arranged by size: 

                 934     620     418     316     298     234     207     127
                  99      81      77      68      12       7       6
 
BspEI T'CCGG_A

Cuts at:      0     458    3504 
   Size:        458    3046
 
BspHI T'CATG_A

Cuts at:      0    1394    1934    3504 
   Size:       1394     540    1570

  Fragments arranged by size: 

                1570    1394     540
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2598    2856    3504 
   Size:       2598     258     648

  Fragments arranged by size: 

                2598     648     258
 
BsrBI CCG'CTC

Cuts at:      0     270     332     337     432    1964    2112    3504 
   Size:        270      62       5      95    1532     148    1392

  Fragments arranged by size: 

                1532    1392     270     148      95      62       5
 
BsrDI GCAATG_nn'

Cuts at:      0    1619    2220    3504 
   Size:       1619     601    1284

  Fragments arranged by size: 

                1619    1284     601
 
BsrFI r'CCGG_y

Cuts at:      0      16      55     803    2229    3504 
   Size:         16      39     748    1426    1275

  Fragments arranged by size: 

                1426    1275     748      39      16
 
BsrGI T'GTAC_A

Cuts at:      0    1057    3504 
   Size:       1057    2447
 
BssHII G'CGCG_C

Cuts at:      0      44    3504 
   Size:         44    3460
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1554    3496    3504 
   Size:       1554    1942       8

  Fragments arranged by size: 

                1942    1554       8
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     541    1014    2751    3504 
   Size:        541     473    1737     753

  Fragments arranged by size: 

                1737     753     541     473
 
BstYI r'GATC_y

Cuts at:      0    1527    1739    1852    3120    3298    3478    3504 
   Size:       1527     212     113    1268     178     180      26

  Fragments arranged by size: 

                1527    1268     212     180     178     113      26
 
Bsu36I CC'TnA_GG

Cuts at:      0    1382    3504 
   Size:       1382    2122
 
BtgI C'CryG_G

Cuts at:      0     293     527    2389    2549    3504 
   Size:        293     234    1862     160     955

  Fragments arranged by size: 

                1862     955     293     234     160
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2528    2892    3504 
   Size:       2528     364     612

  Fragments arranged by size: 

                2528     612     364
 
BtsI GCAGTG_nn'

Cuts at:      0    1548    2354    2738    3089    3350    3504 
   Size:       1548     806     384     351     261     154

  Fragments arranged by size: 

                1548     806     384     351     261     154
 
DraI TTT'AAA

Cuts at:      0    3366    3504 
   Size:       3366     138
 
DraIII CAC_nnn'GTG

Cuts at:      0    1333    3504 
   Size:       1333    2171
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2638    3504 
   Size:       2638     866
 
EaeI y'GGCC_r

Cuts at:      0     181     315     543    1159    2217    3504 
   Size:        181     134     228     616    1058    1287

  Fragments arranged by size: 

                1287    1058     616     228     181     134
 
EagI C'GGCC_G

Cuts at:      0     315    3504 
   Size:        315    3189
 
EarI CTCTTCn'nnn_

Cuts at:      0    1716    1745    1916    2018    2465    2693    2939    3504 
   Size:       1716      29     171     102     447     228     246     565

  Fragments arranged by size: 

                1716     565     447     246     228     171     102      29
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    3446    3504 
   Size:       3446      58
 
EcoICRI GAG'CTC

Cuts at:      0     614    3504 
   Size:        614    2890
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286    1606    1697    1752    1937    1938    2811    2822 
   Size:        286    1320      91      55     185       1     873      11

Cuts at:   2822    3149    3178    3238    3504 
   Size:        327      29      60     266

  Fragments arranged by size: 

                1320     873     327     286     266     185      91      60
                  55      29      11       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1085    1182    1408    3504 
   Size:       1085      97     226    2096

  Fragments arranged by size: 

                2096    1085     226      97
 
EcoO109I rG'GnC_Cy

Cuts at:      0     435    1378    1539    3504 
   Size:        435     943     161    1965

  Fragments arranged by size: 

                1965     943     435     161
 
EcoRI G'AATT_C

Cuts at:      0    2923    3504 
   Size:       2923     581
 
EcoRV GAT'ATC

Cuts at:      0    3167    3504 
   Size:       3167     337
 
HaeII r_GCGC'y

Cuts at:      0      17     259    1519    2639    3504 
   Size:         17     242    1260    1120     865

  Fragments arranged by size: 

                1260    1120     865     242      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1090    1122    1981    2013    2432    2464    3151    3183 
   Size:       1090      32     859      32     419      32     687      32

Cuts at:   3183    3218    3224    3250    3256    3504 
   Size:         35       6      26       6     248

  Fragments arranged by size: 

                1090     859     687     419     248      35      32      32
                  32      32      26       6       6
 
HincII GTy'rAC

Cuts at:      0     629    3146    3504 
   Size:        629    2517     358

  Fragments arranged by size: 

                2517     629     358
 
HindIII A'AGCT_T

Cuts at:      0     825    1207    3504 
   Size:        825     382    2297

  Fragments arranged by size: 

                2297     825     382
 
KasI G'GCGC_C

Cuts at:      0      13    1515    3504 
   Size:         13    1502    1989

  Fragments arranged by size: 

                1989    1502      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    3310    3504 
   Size:        355    2955     194

  Fragments arranged by size: 

                2955     355     194
 
MscI TGG'CCA

Cuts at:      0     545    2219    3504 
   Size:        545    1674    1285

  Fragments arranged by size: 

                1674    1285     545
 
MslI CAynn'nnrTG

Cuts at:      0    1651    1699    1939    2213    2455    2500    2767    2860 
   Size:       1651      48     240     274     242      45     267      93

Cuts at:   2860    3061    3310    3504 
   Size:        201     249     194

  Fragments arranged by size: 

                1651     274     267     249     242     240     201     194
                  93      48      45
 
MspA1I CmG'CkG

Cuts at:      0    1143    1276    1977    2046    2391    2431    2487    2875 
   Size:       1143     133     701      69     345      40      56     388

Cuts at:   2875    3127    3262    3504 
   Size:        252     135     242

  Fragments arranged by size: 

                1143     701     388     345     252     242     135     133
                  69      56      40
 
NaeI GCC'GGC

Cuts at:      0      18      57    2231    3504 
   Size:         18      39    2174    1273

  Fragments arranged by size: 

                2174    1273      39      18
 
NarI GG'CG_CC

Cuts at:      0      14    1516    3504 
   Size:         14    1502    1988

  Fragments arranged by size: 

                1988    1502      14
 
NcoI C'CATG_G

Cuts at:      0     293     527    2549    3504 
   Size:        293     234    2022     955

  Fragments arranged by size: 

                2022     955     293     234
 
NgoMIV G'CCGG_C

Cuts at:      0      16      55    2229    3504 
   Size:         16      39    2174    1275

  Fragments arranged by size: 

                2174    1275      39      16
 
NsiI A_TGCA'T

Cuts at:      0    1194    1241    3504 
   Size:       1194      47    2263

  Fragments arranged by size: 

                2263    1194      47
 
NspI r_CATG'y

Cuts at:      0    1239    1243    1704    2069    2656    3504 
   Size:       1239       4     461     365     587     848

  Fragments arranged by size: 

                1239     848     587     461     365       4
 
PasI CC'CwG_GG

Cuts at:      0    2377    3504 
   Size:       2377    1127
 
PciI A'CATG_T

Cuts at:      0    2652    3504 
   Size:       2652     852
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     528    3504 
   Size:        528    2976
 
PmlI CAC'GTG

Cuts at:      0    1330    2676    3504 
   Size:       1330    1346     828

  Fragments arranged by size: 

                1346    1330     828
 
PpuMI rG'GwC_Cy

Cuts at:      0     435    1539    3504 
   Size:        435    1104    1965

  Fragments arranged by size: 

                1965    1104     435
 
PshAI GACnn'nnGTC

Cuts at:      0    2618    3002    3504 
   Size:       2618     384     502

  Fragments arranged by size: 

                2618     502     384
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1656    1688    3504 
   Size:       1656      32    1816

  Fragments arranged by size: 

                1816    1656      32
 
PstI C_TGCA'G

Cuts at:      0    1514    3504 
   Size:       1514    1990
 
PvuII CAG'CTG

Cuts at:      0    1143    1977    2046    2875    3127    3504 
   Size:       1143     834      69     829     252     377

  Fragments arranged by size: 

                1143     834     829     377     252      69
 
SacI G_AGCT'C

Cuts at:      0     616    3504 
   Size:        616    2888
 
SacII CC_GC'GG

Cuts at:      0    2392    3504 
   Size:       2392    1112
 
SanDI GG'GwC_CC

Cuts at:      0     435    1539    3504 
   Size:        435    1104    1965

  Fragments arranged by size: 

                1965    1104     435
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1745    3504 
   Size:       1745    1759
 
ScaI AGT'ACT

Cuts at:      0    2298    3504 
   Size:       2298    1206
 
SexAI A'CCwGG_T

Cuts at:      0    3411    3504 
   Size:       3411      93
 
SfcI C'TryA_G

Cuts at:      0    1510    2031    3504 
   Size:       1510     521    1473

  Fragments arranged by size: 

                1510    1473     521
 
SfoI GGC'GCC

Cuts at:      0      15    1517    3504 
   Size:         15    1502    1987

  Fragments arranged by size: 

                1987    1502      15
 
SgrAI Cr'CCGG_yG

Cuts at:      0      16      55    3504 
   Size:         16      39    3449

  Fragments arranged by size: 

                3449      39      16
 
SmaI CCC'GGG

Cuts at:      0      86     246     385    3504 
   Size:         86     160     139    3119

  Fragments arranged by size: 

                3119     160     139      86
 
SmlI C'TyrA_G

Cuts at:      0    1000    3504 
   Size:       1000    2504
 
StyI C'CwwG_G

Cuts at:      0     157     293     527    1229    1352    2038    2549    2666 
   Size:        157     136     234     702     123     686     511     117

Cuts at:   2666    2807    3504 
   Size:        141     697

  Fragments arranged by size: 

                 702     697     686     511     234     157     141     136
                 123     117
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     266    1897    2992    3504 
   Size:        266    1631    1095     512

  Fragments arranged by size: 

                1631    1095     512     266
 
TatI w'GTAC_w

Cuts at:      0    1057    2296    2351    2645    3204    3504 
   Size:       1057    1239      55     294     559     300

  Fragments arranged by size: 

                1239    1057     559     300     294      55
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1866    1898    3027    3059    3504 
   Size:       1866      32    1129      32     445

  Fragments arranged by size: 

                1866    1129     445      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2506    3504 
   Size:       2506     998
 
XmaI C'CCGG_G

Cuts at:      0      84     244     383    3504 
   Size:         84     160     139    3121

  Fragments arranged by size: 

                3121     160     139      84
 
XmnI GAAnn'nnTTC

Cuts at:      0    1471    3504 
   Size:       1471    2033
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AloI
    AlwNI    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI     BcgI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII
    Hin4I   HincII  HindIII     KasI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI     PciI
    PflMI     PmlI    PpuMI    PshAI     PsrI     PstI    PvuII     SacI
    SacII    SanDI     SapI     ScaI    SexAI     SfcI     SfoI    SgrAI
     SmaI     SmlI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AlfI     ApaI     AscI
     AseI    AsiSI     BaeI    BbvCI    BciVI     BmtI     BplI   Bpu10I
    BsiWI    BssSI    BstBI   BstEII  BstZ17I     ClaI    CspCI     FalI
     FseI     FspI    FspAI     HpaI     KpnI     MfeI     MluI     NdeI
     NheI     NotI     NruI     PacI     PfoI     PmeI     PpiI     PsiI
   PspOMI    PspXI     PvuI    RsrII     SalI     SbfI     SfiI     SgfI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XhoI     ZraI
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