Restriction map for pF1KB3796
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        AspEI       
AspI        AvrII       BbeI        BcgI        BciVI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BsrBI       BsrGI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT22I     FseI        HaeII       Hin1I       HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspBI       SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AlwNI         945  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       168  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI           83  CCCGAG               C^[CT]CG[GA]G
BamHI         158  GGATCC               G^GATCC
BbvCI        2281  CCTCAGC              CCTCAGC(-5/-2)
BclI          236  TGATCA               T^GATCA
BfaI         2339  CTAG                 C^TAG
BglI         1891  GCCCACTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI        1159  GAGGAG               GAGGAG(10/8)
BsoBI          83  CCCGAG               C^[CT]CG[GA]G
BspLU11I      360  ACATGT               A^CATGT
BssHII          7  GCGCGC               G^CGCGC
BstEII       1995  GGTGACC              G^GT[ACGT]ACC
BstPI        1995  GGTGACC              G^GT[ACGT]ACC
EcoNI        2468  CCTTGGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1995  GGTGACC              G^GT[ACGT]ACC
EcoRI        1988  GAATTC               G^AATTC
FbaI          236  TGATCA               T^GATCA
HindIII       730  AAGCTT               A^AGCTT
MaeI         2339  CTAG                 C^TAG
NaeI         1622  GCCGGC               GCC^GGC
NgoMIV       1622  GCCGGC               G^CCGGC
PvuII        1273  CAGCTG               CAG^CTG
SbfI         1282  CCTGCAGG             CCTGCA^GG
SexAI        1467  ACCAGGT              A^CC[AT]GGT
Sse8387I     1282  CCTGCAGG             CCTGCA^GG
StuI         2172  AGGCCT               AGG^CCT
XmnI          168  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I       1007  GGTACC               G^GTACC
             1636  GGTACC              
AcsI          706  GAATTT               [GA]^AATT[CT]
             1988  GAATTC              
Alw44I        825  GTGCAC               G^TGCAC
             2333  GTGCAC              
ApaLI         825  GTGCAC               G^TGCAC
             2333  GTGCAC              
ApoI          706  GAATTT               [GA]^AATT[CT]
             1988  GAATTC              
Asp718I      1007  GGTACC               G^GTACC
             1636  GGTACC              
AviII         759  TGCGCA               TGC^GCA
             1243  TGCGCA              
BalI         2441  TGGCCA               TGG^CCA
             2471  TGGCCA              
BanII        2324  GGGCTC               G[GA]GC[CT]^C
             2500  GGGCTC              
BsrFI          55  GCCGGT               [GA]^CCGG[CT]
             1622  GCCGGC              
BssSI        1438  CACGAG               CACGAG(-5/-1)
             1825  CACGAG              
Cfr10I         55  GCCGGT               [GA]^CCGG[CT]
             1622  GCCGGC              
FspI          759  TGCGCA               TGC^GCA
             1243  TGCGCA              
HincII       2245  GTTGAC               GT[CT]^[GA]AC
             2292  GTCAAC              
HindII       2245  GTTGAC               GT[CT]^[GA]AC
             2292  GTCAAC              
KpnI         1007  GGTACC               GGTAC^C
             1636  GGTACC              
MluNI        2441  TGGCCA               TGG^CCA
             2471  TGGCCA              
MscI         2441  TGGCCA               TGG^CCA
             2471  TGGCCA              
NcoI          354  CCATGG               C^CATGG
             1019  CCATGG              
PflMI        1627  CCAGTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2122  CCACCCCGTGG         
SapI          110  GAAGAGC              GCTCTTC(1/4)
              168  GAAGAGC             
SspI          205  AATATT               AAT^ATT
             1804  AATATT              
Van91I       1627  CCAGTCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2122  CCACCCCGTGG         
XcmI          794  CCACCATCGGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1982  CCATAGGAATTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AspHI       AvaII       BanI        BbrPI       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PmaCI       PmlI        PpuMI       PstI        
RcaI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com