Restriction map for pF1KB3796
 (Linear) (Six-base) MAPSORT of: KIBB3796.seq  Check: 4814  from: 1  to: 2580



KIBB3796.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1507    2580 
   Size:       1507    1073
 
Acc65I G'GTAC_C

Cuts at:      0    1007    1636    2580 
   Size:       1007     629     944

  Fragments arranged by size: 

                1007     944     629
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1246    1297    2580 
   Size:       1246      51    1283

  Fragments arranged by size: 

                1283    1246      51
 
AflIII A'CryG_T

Cuts at:      0     360     400    1367    1709    2580 
   Size:        360      40     967     342     871

  Fragments arranged by size: 

                 967     871     360     342      40
 
AleI CACnn'nnGTG

Cuts at:      0    1829    2580 
   Size:       1829     751
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1857    2580 
   Size:       1857     723
 
AlwNI CAG_nnn'CTG

Cuts at:      0     950    2580 
   Size:        950    1630
 
ApaLI G'TGCA_C

Cuts at:      0     825    2333    2580 
   Size:        825    1508     247

  Fragments arranged by size: 

                1508     825     247
 
ApoI r'AATT_y

Cuts at:      0     706    1988    2580 
   Size:        706    1282     592

  Fragments arranged by size: 

                1282     706     592
 
AvaI C'yCGr_G

Cuts at:      0      83    2580 
   Size:         83    2497
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     299     332    2580 
   Size:        299      33    2248

  Fragments arranged by size: 

                2248     299      33
 
BamHI G'GATC_C

Cuts at:      0     158    2580 
   Size:        158    2422
 
BanI G'GyrC_C

Cuts at:      0    1007    1615    1636    1899    2511    2580 
   Size:       1007     608      21     263     612      69

  Fragments arranged by size: 

                1007     612     608     263      69      21
 
BanII G_rGCy'C

Cuts at:      0    2328    2504    2580 
   Size:       2328     176      76

  Fragments arranged by size: 

                2328     176      76
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1271    2002    2053    2086    2580 
   Size:       1271     731      51      33     494

  Fragments arranged by size: 

                1271     731     494      51      33
 
BbvCI CC'TCA_GC

Cuts at:      0    2282    2580 
   Size:       2282     298
 
BclI T'GATC_A

Cuts at:      0     236    2580 
   Size:        236    2344
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1897    2580 
   Size:       1897     683
 
Bme1580I G_kGCm'C

Cuts at:      0     829    1388    1902    2337    2552    2580 
   Size:        829     559     514     435     215      28

  Fragments arranged by size: 

                 829     559     514     435     215      28
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     157    1905    2283    2507    2580 
   Size:        157    1748     378     224      73

  Fragments arranged by size: 

                1748     378     224     157      73
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     738    1084    1290    2580 
   Size:        738     346     206    1290

  Fragments arranged by size: 

                1290     738     346     206
 
Bpu10I CC'TnA_GC

Cuts at:      0    2282    2580 
   Size:       2282     298
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     268     415    1048    2580 
   Size:        268     147     633    1532

  Fragments arranged by size: 

                1532     633     268     147
 
BsaAI yAC'GTr

Cuts at:      0     401    1710    2332    2580 
   Size:        401    1309     622     248

  Fragments arranged by size: 

                1309     622     401     248
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1174    2580 
   Size:       1174    1406
 
BseYI C'CCAG_C

Cuts at:      0     492     906    1359    2580 
   Size:        492     414     453    1221

  Fragments arranged by size: 

                1221     492     453     414
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     902    1317    1320    1360    1787    2357    2580 
   Size:        902     415       3      40     427     570     223

  Fragments arranged by size: 

                 902     570     427     415     223      40       3
 
BsiHKAI G_wGCw'C

Cuts at:      0      63     829    2337    2580 
   Size:         63     766    1508     243

  Fragments arranged by size: 

                1508     766     243      63
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     829    1388    1902    2328    2337    2504    2552 
   Size:         63     766     559     514     426       9     167      48

Cuts at:   2552    2580 
   Size:         28

  Fragments arranged by size: 

                 766     559     514     426     167      63      48      28
                   9
 
BspHI T'CATG_A

Cuts at:      0     815     930     989    2580 
   Size:        815     115      59    1591

  Fragments arranged by size: 

                1591     815     115      59
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1103    1382    1488    1507    2532    2580 
   Size:       1103     279     106      19    1025      48

  Fragments arranged by size: 

                1103    1025     279     106      48      19
 
BsrDI GCAATG_nn'

Cuts at:      0     266    1212    1841    2580 
   Size:        266     946     629     739

  Fragments arranged by size: 

                 946     739     629     266
 
BsrFI r'CCGG_y

Cuts at:      0      55    1622    2580 
   Size:         55    1567     958

  Fragments arranged by size: 

                1567     958      55
 
BssHII G'CGCG_C

Cuts at:      0       7    2580 
   Size:          7    2573
 
BssSI C'ACGA_G

Cuts at:      0    1438    1825    2580 
   Size:       1438     387     755

  Fragments arranged by size: 

                1438     755     387
 
BstEII G'GTnAC_C

Cuts at:      0    1995    2580 
   Size:       1995     585
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     804    1132    2489    2580 
   Size:        804     328    1357      91

  Fragments arranged by size: 

                1357     804     328      91
 
BstYI r'GATC_y

Cuts at:      0     158    1490    2433    2554    2580 
   Size:        158    1332     943     121      26

  Fragments arranged by size: 

                1332     943     158     121      26
 
BtgI C'CryG_G

Cuts at:      0     354    1019    1845    2127    2580 
   Size:        354     665     826     282     453

  Fragments arranged by size: 

                 826     665     453     354     282
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1736    2580 
   Size:       1736     844
 
BtsI GCAGTG_nn'

Cuts at:      0    1353    1453    1846    1881    2239    2580 
   Size:       1353     100     393      35     358     341

  Fragments arranged by size: 

                1353     393     358     341     100      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1763    2128    2547    2580 
   Size:       1763     365     419      33

  Fragments arranged by size: 

                1763     419     365      33
 
EaeI y'GGCC_r

Cuts at:      0      53    1624    2119    2157    2441    2471    2580 
   Size:         53    1571     495      38     284      30     109

  Fragments arranged by size: 

                1571     495     284     109      53      38      30
 
EarI CTCTTCn'nnn_

Cuts at:      0     105     163     493    1546    2580 
   Size:        105      58     330    1053    1034

  Fragments arranged by size: 

                1053    1034     330     105      58
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2494    2580 
   Size:       2494      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     738    1084    1246    1290    1297    2580 
   Size:        738     346     162      44       7    1283

  Fragments arranged by size: 

                1283     738     346     162      44       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2472    2580 
   Size:       2472     108
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     684    1703    2393    2477    2580 
   Size:        150     534    1019     690      84     103

  Fragments arranged by size: 

                1019     690     534     150     103      84
 
EcoRI G'AATT_C

Cuts at:      0    1988    2580 
   Size:       1988     592
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1575    1941    2580 
   Size:       1575     366     639

  Fragments arranged by size: 

                1575     639     366
 
FspI TGC'GCA

Cuts at:      0     761    1245    2580 
   Size:        761     484    1335

  Fragments arranged by size: 

                1335     761     484
 
FspAI rTGC'GCAy

Cuts at:      0    1245    2580 
   Size:       1245    1335
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     228     260     470     502     655     687     971    1003 
   Size:        228      32     210      32     153      32     284      32

Cuts at:   1003    1372    1404    2580 
   Size:        369      32    1176

  Fragments arranged by size: 

                1176     369     284     228     210     153      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2247    2294    2580 
   Size:       2247      47     286

  Fragments arranged by size: 

                2247     286      47
 
HindIII A'AGCT_T

Cuts at:      0     730    2580 
   Size:        730    1850
 
KpnI G_GTAC'C

Cuts at:      0    1011    1640    2580 
   Size:       1011     629     940

  Fragments arranged by size: 

                1011     940     629
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2160    2580 
   Size:       2160     420
 
MscI TGG'CCA

Cuts at:      0    2443    2473    2580 
   Size:       2443      30     107

  Fragments arranged by size: 

                2443     107      30
 
MslI CAynn'nnrTG

Cuts at:      0     359     755     802     820    1829    2580 
   Size:        359     396      47      18    1009     751

  Fragments arranged by size: 

                1009     751     396     359      47      18
 
MspA1I CmG'CkG

Cuts at:      0      15    1275    1946    2580 
   Size:         15    1260     671     634

  Fragments arranged by size: 

                1260     671     634      15
 
NaeI GCC'GGC

Cuts at:      0    1624    2580 
   Size:       1624     956
 
NcoI C'CATG_G

Cuts at:      0     354    1019    2580 
   Size:        354     665    1561

  Fragments arranged by size: 

                1561     665     354
 
NgoMIV G'CCGG_C

Cuts at:      0    1622    2580 
   Size:       1622     958
 
NspI r_CATG'y

Cuts at:      0     364     825    1731    2580 
   Size:        364     461     906     849

  Fragments arranged by size: 

                 906     849     461     364
 
PasI CC'CwG_GG

Cuts at:      0    1486    1738    2388    2580 
   Size:       1486     252     650     192

  Fragments arranged by size: 

                1486     650     252     192
 
PciI A'CATG_T

Cuts at:      0     360    2580 
   Size:        360    2220
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1633    2128    2580 
   Size:       1633     495     452

  Fragments arranged by size: 

                1633     495     452
 
PmlI CAC'GTG

Cuts at:      0     401    1710    2332    2580 
   Size:        401    1309     622     248

  Fragments arranged by size: 

                1309     622     401     248
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     684    1703    2393    2477    2580 
   Size:        150     534    1019     690      84     103

  Fragments arranged by size: 

                1019     690     534     150     103      84
 
PstI C_TGCA'G

Cuts at:      0    1287    1518    1676    2234    2433    2580 
   Size:       1287     231     158     558     199     147

  Fragments arranged by size: 

                1287     558     231     199     158     147
 
PvuII CAG'CTG

Cuts at:      0    1275    2580 
   Size:       1275    1305
 
SanDI GG'GwC_CC

Cuts at:      0    1703    2580 
   Size:       1703     877
 
SapI GCTCTTCn'nnn_

Cuts at:      0     105     163    2580 
   Size:        105      58    2417

  Fragments arranged by size: 

                2417     105      58
 
SbfI CC_TGCA'GG

Cuts at:      0    1287    2580 
   Size:       1287    1293
 
SexAI A'CCwGG_T

Cuts at:      0    1467    2580 
   Size:       1467    1113
 
SfcI C'TryA_G

Cuts at:      0     951    1283    1514    1672    2230    2429    2580 
   Size:        951     332     231     158     558     199     151

  Fragments arranged by size: 

                 951     558     332     231     199     158     151
 
SspI AAT'ATT

Cuts at:      0     207    1806    2580 
   Size:        207    1599     774

  Fragments arranged by size: 

                1599     774     207
 
StuI AGG'CCT

Cuts at:      0    2174    2580 
   Size:       2174     406
 
StyI C'CwwG_G

Cuts at:      0     105     354     786     876    1019    1971    2163    2468 
   Size:        105     249     432      90     143     952     192     305

Cuts at:   2468    2580 
   Size:        112

  Fragments arranged by size: 

                 952     432     305     249     192     143     112     105
                  90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2575    2580 
   Size:       2575       5
 
TatI w'GTAC_w

Cuts at:      0    1712    2580 
   Size:       1712     868
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     410     442    1064    1096    1225    1257    1979    2011 
   Size:        410      32     622      32     129      32     722      32

Cuts at:   2011    2327    2359    2580 
   Size:        316      32     221

  Fragments arranged by size: 

                 722     622     410     316     221     129      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     801    1989    2580 
   Size:        801    1188     591

  Fragments arranged by size: 

                1188     801     591
 
XmnI GAAnn'nnTTC

Cuts at:      0     172    2580 
   Size:        172    2408
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AleI     AlfI    AlwNI    ApaLI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI
     BclI     BglI Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI   BssHII    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    FspAI    Hin4I   HincII  HindIII     KpnI
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI     PciI    PflMI     PmlI    PpuMI     PstI    PvuII    SanDI
     SapI     SbfI    SexAI     SfcI     SspI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI     AscI     AseI    AsiSI    AvrII     BbeI     BcgI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI    BsrGI   BstAPI    BstBI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI     DrdI     EagI  EcoICRI    EcoRV     FseI    HaeII     HpaI
     KasI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PfoI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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