Restriction map for pF1KB3765
##################################
# NO CUT ENZYMES (  136 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BbrPI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HgaI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MspA1I      MunI        MvnI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfuI        SgrAI       
SmaI        SmlI        SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TfiI        Tth111I     
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AciI         1001  GCGG                 CCGC(-3/-1)
AclI          568  AACGTT               AA^CGTT
AcyI          232  GGCGCC               G[GA]^CG[CT]C
AseI         1242  ATTAAT               AT^TAAT
AsnI         1242  ATTAAT               AT^TAAT
Asp700I       626  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         109  GAGCAC               G[AT]GC[AT]^C
BalI          132  TGGCCA               TGG^CCA
BanI          232  GGCGCC               G^G[CT][GA]CC
BanII         383  GAGCCC               G[GA]GC[CT]^C
BbeI          232  GGCGCC               GGCGC^C
BbsI         1114  GAAGAC               GAAGAC(2/6)
BbvCI        1187  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1097  GCAGTATCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1042  CTAG                 C^TAG
BglI          691  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          661  CTCCAG               CTGGAG(16/14)
BpuAI        1114  GAAGAC               GAAGAC(2/6)
BsaAI          77  TACGTA               [CT]AC^GT[GA]
BsaHI         232  GGCGCC               G[GA]^CG[CT]C
BsaI          902  GAGACC               GGTCTC(1/5)
BsiHKAI       109  GAGCAC               G[AT]GC[AT]^C
Bsp1407I     1356  TGTACA               T^GTACA
BsrDI        1292  CATTGC               GCAATG(2/0)
BsrGI        1356  TGTACA               T^GTACA
BssSI        1070  CACGAG               CACGAG(-5/-1)
CfoI          233  GCGC                 GCG^C
DraI          556  TTTAAA               TTT^AAA
DraII        1044  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EaeI          132  TGGCCA               [CT]^GGCC[GA]
EarI         1301  CTCTTC               CTCTTC(1/4)
EcoO109I     1044  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       129  CCTTGG               C^C[AT][AT]GG
FauI         1001  GCGGG                CCCGC(4/6)
HaeII         232  GGCGCC               [GA]GCGC^[CT]
HhaI          233  GCGC                 GCG^C
Hin1I         232  GGCGCC               G[GA]^CG[CT]C
HinP1I        233  GCGC                 G^CGC
KasI          232  GGCGCC               G^GCGCC
Ksp632I      1301  CTCTTC               CTCTTC(1/4)
MaeI         1042  CTAG                 C^TAG
MluNI         132  TGGCCA               TGG^CCA
MscI          132  TGGCCA               TGG^CCA
MslI          276  CATGGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          232  GGCGCC               GG^CGCC
NspI          240  GCATGT               [GA]CATG^[CT]
PflMI         635  CCATTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1242  ATTAAT               AT^TAAT
Psp1406I      568  AACGTT               AA^CGTT
SapI         1300  GCTCTTC              GCTCTTC(1/4)
SfcI         1164  CTACAG               C^T[GA][CT]AG
SfoI          232  GGCGCC               GGC^GCC
SnaBI          77  TACGTA               TAC^GTA
SspBI        1356  TGTACA               T^GTACA
StyI          129  CCTTGG               C^C[AT][AT]GG
TaqI          657  TCGA                 T^CGA
TthHB8I       657  TCGA                 T^CGA
Van91I        635  CCATTCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          626  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          609  GAATTT               [GA]^AATT[CT]
              933  AAATTC              
ApoI          609  GAATTT               [GA]^AATT[CT]
              933  AAATTC              
BciVI         925  GTATCC               GTATCC(6/5)
             1100  GTATCC              
BclI          245  TGATCA               T^GATCA
              416  TGATCA              
BseRI         864  CTCCTC               GAGGAG(10/8)
              211  GAGGAG              
BsgI           14  CTGCAC               GTGCAG(16/14)
              388  CTGCAC              
BsmFI        1031  GGGAC                GGGAC(10/14)
              259  GTCCC               
BspHI         413  TCATGA               T^CATGA
              434  TCATGA              
BspMI          12  ACCTGC               ACCTGC(4/8)
              180  GCAGGT              
BstF5I        483  CATCC                GGATG(2/0)
              327  GGATG               
Cac8I         666  GCAGGC               GC[ACGT]^[ACGT]GC
             1296  GCCAGC              
FbaI          245  TGATCA               T^GATCA
              416  TGATCA              
FokI          483  CATCC                GGATG(9/13)
              327  GGATG               
MaeII          78  ACGT                 A^CGT
              569  ACGT                
MwoI          691  GCCCTCATGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1291  GCATTGCCAGC         
NlaIV         125  GGAGCC               GG[ACGT]^[ACGT]CC
              232  GGCGCC              
RcaI          413  TCATGA               T^CATGA
              434  TCATGA              


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AfaI        AluI        AvaII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmI        Bsp1286I    BsrI        BssKI       BstNI       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MnlI        
MseI        MspI        MvaI        NciI        NdeII       
NlaIII      PleI        RsaI        Sau3AI      Sau96I      
ScrFI       Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       
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