Restriction map for pF1KB3736
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AfaI        
AflII       AflIII      AgeI        Alw44I      AocI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvaI        AvrII       BamHI       
BanI        BbeI        BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BsaBI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SgrAI       SmaI        SmlI        SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          679  GTCTAC               GT^[AC][GT]AC
AccII         706  CGCG                 CG^CG
AcsI          223  AAATTT               [GA]^AATT[CT]
AcyI          207  GACGCC               G[GA]^CG[CT]C
AhdI         1161  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1041  GATCC                GGATC(4/5)
AlwNI         883  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       416  AGCGCT               AGC^GCT
ApaI          809  GGGCCC               GGGCC^C
ApoI          223  AAATTT               [GA]^AATT[CT]
AspEI        1161  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         455  TGCGCA               TGC^GCA
BbrPI         738  CACGTG               CAC^GTG
BfaI         1112  CTAG                 C^TAG
BsaHI         207  GACGCC               G[GA]^CG[CT]C
BsaWI         830  ACCGGA               [AT]^CCGG[AT]
BsrBI         783  CCGCTC               CCGCTC(-3/-3)
BsrDI         176  GCAATG               GCAATG(2/0)
BsrFI        1034  ACCGGC               [GA]^CCGG[CT]
BstBI        1235  TTCGAA               TT^CGAA
BstUI         706  CGCG                 CG^CG
BstYI        1040  AGATCC               [GA]^GATC[CT]
Cfr10I       1034  ACCGGC               [GA]^CCGG[CT]
DsaI          515  CCATGG               C^C[GA][CT]GG
Eam1105I     1161  GACTGGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      416  AGCGCT               AGC^GCT
FauI          704  CCCGC                CCCGC(4/6)
FspI          455  TGCGCA               TGC^GCA
HaeII         416  AGCGCT               [GA]GCGC^[CT]
Hin1I         207  GACGCC               G[GA]^CG[CT]C
HincII       1228  GTTGAC               GT[CT]^[GA]AC
HindII       1228  GTTGAC               GT[CT]^[GA]AC
MaeI         1112  CTAG                 C^TAG
MflI         1040  AGATCC               [GA]^GATC[CT]
MvnI          706  CGCG                 CG^CG
NcoI          515  CCATGG               C^CATGG
NspI          689  GCATGT               [GA]CATG^[CT]
NspV         1235  TTCGAA               TT^CGAA
PmaCI         738  CACGTG               CAC^GTG
PmlI          738  CACGTG               CAC^GTG
SfuI         1235  TTCGAA               TT^CGAA
SnaBI         427  TACGTA               TAC^GTA
StuI          506  AGGCCT               AGG^CCT
TaqI         1236  TCGA                 T^CGA
Tsp509I       224  AATT                 ^AATT
TthHB8I      1236  TCGA                 T^CGA
XcmI          318  CCACGCCCTCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1040  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

BbsI          229  GTCTTC               GAAGAC(2/6)
              235  GTCTTC              
BbvCI        1101  GCTGAGG              CCTCAGC(-5/-2)
             1255  GCTGAGG             
BglI          703  GCCCGCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1115  GCCGGGAGGGC         
BlpI          940  GCTCAGC              GC^T[ACGT]AGC
             1053  GCTCAGC             
Bpu1102I      940  GCTCAGC              GC^T[ACGT]AGC
             1053  GCTCAGC             
BpuAI         229  GTCTTC               GAAGAC(2/6)
              235  GTCTTC              
BsaAI         427  TACGTA               [CT]AC^GT[GA]
              738  CACGTG              
BsaI         1261  GAGACC               GGTCTC(1/5)
              543  GGTCTC              
BstEII        795  GGTCACC              G^GT[ACGT]ACC
              975  GGTCACC             
BstPI         795  GGTCACC              G^GT[ACGT]ACC
              975  GGTCACC             
BstXI         404  CCATCAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              737  CCACGTGGATGG        
CelII         940  GCTCAGC              GC^T[ACGT]AGC
             1053  GCTCAGC             
DpnII         198  GATC                 ^GATC
             1041  GATC                
EarI           36  CTCTTC               CTCTTC(1/4)
              136  CTCTTC              
EcoO65I       795  GGTCACC              G^GT[ACGT]ACC
              975  GGTCACC             
EcoT14I       509  CCTTGG               C^C[AT][AT]GG
              515  CCATGG              
HgaI          207  GACGC                GACGC(5/10)
              462  GACGC               
Ksp632I        36  CTCTTC               CTCTTC(1/4)
              136  CTCTTC              
MaeIII        796  GTCAC                ^GT[ACGT]AC
              976  GTCAC               
MboI          198  GATC                 ^GATC
             1041  GATC                
MspA1I        182  CCGCTG               C[AC]G^C[GT]G
              730  CCGCTG              
NdeII         198  GATC                 ^GATC
             1041  GATC                
PstI          684  CTGCAG               CTGCA^G
             1144  CTGCAG              
SacI          560  GAGCTC               GAGCT^C
             1051  GAGCTC              
Sau3AI        198  GATC                 ^GATC
             1041  GATC                
SfcI          684  CTGCAG               C^T[GA][CT]AG
             1144  CTGCAG              
StyI          509  CCTTGG               C^C[AT][AT]GG
              515  CCATGG              
Tsp45I        796  GTCAC                ^GT[GC]AC
              976  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AluI        AspHI       AvaII       BalI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EaeI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MboII       MluNI       MnlI        
MscI        MslI        MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PleI        PpuMI       
Sau96I      ScrFI       TfiI        TseI        TspRI       
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