Restriction map for pF1KB3736
 (Linear) (Six-base) MAPSORT of: KIBB3736.seq  Check: 4007  from: 1  to: 1353



KIBB3736.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     951    1230    1353 
   Size:        951     279     123

  Fragments arranged by size: 

                 951     279     123
 
AccI GT'mk_AC

Cuts at:      0     680    1353 
   Size:        680     673
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1138    1353 
   Size:       1138     215
 
AfeI AGC'GCT

Cuts at:      0     418    1353 
   Size:        418     935
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1166    1353 
   Size:       1166     187
 
AleI CACnn'nnGTG

Cuts at:      0     591    1353 
   Size:        591     762
 
AlwNI CAG_nnn'CTG

Cuts at:      0     888    1353 
   Size:        888     465
 
ApaI G_GGCC'C

Cuts at:      0     813    1353 
   Size:        813     540
 
ApoI r'AATT_y

Cuts at:      0     223    1353 
   Size:        223    1130
 
BanII G_rGCy'C

Cuts at:      0     564     813    1055    1126    1353 
   Size:        564     249     242      71     227

  Fragments arranged by size: 

                 564     249     242     227      71
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     222     228    1353 
   Size:        222       6    1125

  Fragments arranged by size: 

                1125     222       6
 
BbvCI CC'TCA_GC

Cuts at:      0    1102    1256    1353 
   Size:       1102     154      97

  Fragments arranged by size: 

                1102     154      97
 
BglI GCCn_nnn'nGGC

Cuts at:      0     709    1121    1353 
   Size:        709     412     232

  Fragments arranged by size: 

                 709     412     232
 
BlpI GC'TnA_GC

Cuts at:      0     941    1054    1353 
   Size:        941     113     299

  Fragments arranged by size: 

                 941     299     113
 
Bme1580I G_kGCm'C

Cuts at:      0     813    1353 
   Size:        813     540
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      15     560     965    1353 
   Size:         15     545     405     388

  Fragments arranged by size: 

                 545     405     388      15
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      74    1281    1353 
   Size:         74    1207      72

  Fragments arranged by size: 

                1207      74      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     574    1073    1297    1353 
   Size:        282     292     499     224      56

  Fragments arranged by size: 

                 499     292     282     224      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     804    1102    1256    1353 
   Size:        804     298     154      97

  Fragments arranged by size: 

                 804     298     154      97
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     549    1255    1353 
   Size:        549     706      98

  Fragments arranged by size: 

                 706     549      98
 
BsaAI yAC'GTr

Cuts at:      0     429     740    1353 
   Size:        429     311     613

  Fragments arranged by size: 

                 613     429     311
 
BsaHI Gr'CG_yC

Cuts at:      0     208    1353 
   Size:        208    1145
 
BsaWI w'CCGG_w

Cuts at:      0     830    1353 
   Size:        830     523
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     588     618    1353 
   Size:        588      30     735

  Fragments arranged by size: 

                 735     588      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     655    1108    1111    1147    1273    1353 
   Size:        655     453       3      36     126      80

  Fragments arranged by size: 

                 655     453     126      80      36       3
 
BseYI C'CCAG_C

Cuts at:      0     465    1201    1353 
   Size:        465     736     152

  Fragments arranged by size: 

                 736     465     152
 
BsiHKAI G_wGCw'C

Cuts at:      0     564     942    1055    1353 
   Size:        564     378     113     298

  Fragments arranged by size: 

                 564     378     298     113
 
Bsp1286I G_dGCh'C

Cuts at:      0     564     813     942    1055    1126    1353 
   Size:        564     249     129     113      71     227

  Fragments arranged by size: 

                 564     249     227     129     113      71
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     266     951    1230    1353 
   Size:        266     685     279     123

  Fragments arranged by size: 

                 685     279     266     123
 
BsrBI CCG'CTC

Cuts at:      0     785    1353 
   Size:        785     568
 
BsrDI GCAATG_nn'

Cuts at:      0     183    1353 
   Size:        183    1170
 
BsrFI r'CCGG_y

Cuts at:      0    1034    1353 
   Size:       1034     319
 
BstBI TT'CG_AA

Cuts at:      0    1236    1353 
   Size:       1236     117
 
BstEII G'GTnAC_C

Cuts at:      0     795     975    1353 
   Size:        795     180     378

  Fragments arranged by size: 

                 795     378     180
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     411     744    1353 
   Size:        411     333     609

  Fragments arranged by size: 

                 609     411     333
 
BstYI r'GATC_y

Cuts at:      0    1040    1353 
   Size:       1040     313
 
BtgI C'CryG_G

Cuts at:      0     515    1353 
   Size:        515     838
 
BtsI GCAGTG_nn'

Cuts at:      0     863    1353 
   Size:        863     490
 
EaeI y'GGCC_r

Cuts at:      0     191     287     383     512    1353 
   Size:        191      96      96     129     841

  Fragments arranged by size: 

                 841     191     129      96      96
 
EarI CTCTTCn'nnn_

Cuts at:      0      42     142    1353 
   Size:         42     100    1211

  Fragments arranged by size: 

                1211     100      42
 
EcoICRI GAG'CTC

Cuts at:      0     562    1053    1353 
   Size:        562     491     300

  Fragments arranged by size: 

                 562     491     300
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     574    1073    1138    1297    1353 
   Size:        282     292     499      65     159      56

  Fragments arranged by size: 

                 499     292     282     159      65      56
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54      70      90     809     810    1353 
   Size:         54      16      20     719       1     543

  Fragments arranged by size: 

                 719     543      54      20      16       1
 
FspI TGC'GCA

Cuts at:      0     457    1353 
   Size:        457     896
 
FspAI rTGC'GCAy

Cuts at:      0     457    1353 
   Size:        457     896
 
HaeII r_GCGC'y

Cuts at:      0     420    1353 
   Size:        420     933
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1152    1184    1353 
   Size:       1152      32     169

  Fragments arranged by size: 

                1152     169      32
 
HincII GTy'rAC

Cuts at:      0    1230    1353 
   Size:       1230     123
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1031    1353 
   Size:       1031     322
 
MscI TGG'CCA

Cuts at:      0     289     385     514    1353 
   Size:        289      96     129     839

  Fragments arranged by size: 

                 839     289     129      96
 
MslI CAynn'nnrTG

Cuts at:      0     409     591     673     742     923    1303    1353 
   Size:        409     182      82      69     181     380      50

  Fragments arranged by size: 

                 409     380     182     181      82      69      50
 
MspA1I CmG'CkG

Cuts at:      0     184     732    1353 
   Size:        184     548     621

  Fragments arranged by size: 

                 621     548     184
 
NcoI C'CATG_G

Cuts at:      0     515    1353 
   Size:        515     838
 
NspI r_CATG'y

Cuts at:      0     693    1353 
   Size:        693     660
 
PfoI T'CCnGG_A

Cuts at:      0      92    1274    1353 
   Size:         92    1182      79

  Fragments arranged by size: 

                1182      92      79
 
PmlI CAC'GTG

Cuts at:      0     740    1353 
   Size:        740     613
 
PpuMI rG'GwC_Cy

Cuts at:      0      54      70      90    1353 
   Size:         54      16      20    1263

  Fragments arranged by size: 

                1263      54      20      16
 
PspOMI G'GGCC_C

Cuts at:      0     809    1353 
   Size:        809     544
 
PstI C_TGCA'G

Cuts at:      0     688    1148    1353 
   Size:        688     460     205

  Fragments arranged by size: 

                 688     460     205
 
SacI G_AGCT'C

Cuts at:      0     564    1055    1353 
   Size:        564     491     298

  Fragments arranged by size: 

                 564     491     298
 
SanDI GG'GwC_CC

Cuts at:      0      54      90    1353 
   Size:         54      36    1263

  Fragments arranged by size: 

                1263      54      36
 
SfcI C'TryA_G

Cuts at:      0     684    1144    1353 
   Size:        684     460     209

  Fragments arranged by size: 

                 684     460     209
 
SnaBI TAC'GTA

Cuts at:      0     429    1353 
   Size:        429     924
 
StuI AGG'CCT

Cuts at:      0     508    1353 
   Size:        508     845
 
StyI C'CwwG_G

Cuts at:      0     509     515    1353 
   Size:        509       6     838

  Fragments arranged by size: 

                 838     509       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      27     481     489    1353 
   Size:         27     454       8     864

  Fragments arranged by size: 

                 864     454      27       8
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     588     620    1353 
   Size:        588      32     733

  Fragments arranged by size: 

                 733     588      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     325    1353 
   Size:        325    1028
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI     AhdI     AleI    AlwNI     ApaI
     ApoI    BanII     BbsI    BbvCI     BglI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BstBI   BstEII    BstXI    BstYI     BtgI     BtsI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I     FspI    FspAI    HaeII    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PfoI     PmlI
    PpuMI   PspOMI     PstI     SacI    SanDI     SfcI    SnaBI     StuI
     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmtI    BpuEI    BsaBI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FalI     FseI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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