Restriction map for pF1KB3734
##################################
# NO CUT ENZYMES (  153 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BanI        BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmBI       BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     Van91I      XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII          28  CGCG                 CG^CG
AvaI         1229  CTCGGG               C^[CT]CG[GA]G
BamHI          96  GGATCC               G^GATCC
BbvI          912  GCTGC                GCAGC(8/12)
BcnI          351  CCGGG                CC^[GC]GG
BglII         341  AGATCT               A^GATCT
BsaBI        1084  GATATCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         217  GAGGAG               GAGGAG(10/8)
BsmAI         963  GAGAC                GTCTC(1/5)
BsmFI         253  GGGAC                GGGAC(10/14)
BsmI         1135  GCATTC               GAATGC(1/-1)
BsoBI        1229  CTCGGG               C^[CT]CG[GA]G
BspHI         810  TCATGA               T^CATGA
BspMI        1046  ACCTGC               ACCTGC(4/8)
BstUI          28  CGCG                 CG^CG
DraII         253  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII       1207  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          395  CCGTGG               C^C[GA][CT]GG
EaeI         1187  TGGCCG               [CT]^GGCC[GA]
EcoO109I      253  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRV        1084  GATATC               GAT^ATC
EcoT14I      1307  CCTTGG               C^C[AT][AT]GG
FauI          237  GCGGG                CCCGC(4/6)
Fnu4HI        912  GCTGC                GC^[ACGT]GC
HaeIII       1188  GGCC                 GG^CC
HapII         351  CCGG                 C^CGG
HpaII         351  CCGG                 C^CGG
ItaI          912  GCTGC                GC^[ACGT]GC
MaeIII        689  GTCAC                ^GT[ACGT]AC
MamI         1084  GATATCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I        910  CAGCTG               C[AC]G^C[GT]G
MspI          351  CCGG                 C^CGG
MvnI           28  CGCG                 CG^CG
NciI          351  CCGGG                CC^[GC]GG
PleI         1324  GACTC                GAGTC(4/5)
PpuMI         253  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII         910  CAGCTG               CAG^CTG
RcaI          810  TCATGA               T^CATGA
SacI          403  GAGCTC               GAGCT^C
SexAI        1069  ACCTGGT              A^CC[AT]GGT
SfcI         1218  CTACAG               C^T[GA][CT]AG
StyI         1307  CCTTGG               C^C[AT][AT]GG
TseI          912  GCTGC                G^C[AT]GC
Tsp45I        689  GTCAC                ^GT[GC]AC
XbaI          828  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AspHI         403  GAGCTC               G[AT]GC[AT]^C
             1285  GTGCTC              
BanII         403  GAGCTC               G[GA]GC[CT]^C
             1249  GAGCCC              
BpmI          101  CTCCAG               CTGGAG(16/14)
               42  CTGGAG              
BsiHKAI       403  GAGCTC               G[AT]GC[AT]^C
             1285  GTGCTC              
Bsp1407I        2  TGTACA               T^GTACA
             1262  TGTACA              
BsrGI           2  TGTACA               T^GTACA
             1262  TGTACA              
BstNI         618  CCAGG                CC^[AT]GG
             1070  CCTGG               
Cac8I        1174  GCTTGC               GC[ACGT]^[ACGT]GC
             1341  GCTTGC              
EcoRII        618  CCAGG                ^CC[AT]GG
             1070  CCTGG               
HgaI           34  GACGC                GACGC(5/10)
              825  GCGTC               
HindIII       650  AAGCTT               A^AGCTT
              851  AAGCTT              
MaeII         795  ACGT                 A^CGT
             1334  ACGT                
MslI          349  CACCGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1054  CATTATGATG          
MvaI          618  CCAGG                CC^[AT]GG
             1070  CCTGG               
NlaIV          96  GGATCC               GG[ACGT]^[ACGT]CC
              253  GGGACC              
SapI          679  GAAGAGC              GCTCTTC(1/4)
              106  GCTCTTC             
SspBI           2  TGTACA               T^GTACA
             1262  TGTACA              
SspI          517  AATATT               AAT^ATT
              672  AATATT              
TaqI           67  TCGA                 T^CGA
             1092  TCGA                
TfiI          427  GAATC                G^A[AT]TC
              807  GATTC               
TthHB8I        67  TCGA                 T^CGA
             1092  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BfaI        BmyI        BsaJI       Bsp1286I    BsrI        
BssKI       BstF5I      BstYI       Cfr13I      DdeI        
DpnII       EarI        FokI        HinfI       HphI        
Ksp632I     MaeI        MboI        MboII       MflI        
MnlI        MseI        MwoI        NdeII       NlaIII      
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
Tru9I       Tsp509I     TspRI       XhoII       
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