Restriction map for pF1KB3717
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
Aor51HI     ApaI        ApoI        AscI        Asp700I     
Asp718I     AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BssHII      BssSI       BstAPI      
BstUI       CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoT22I     FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SwaI        Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AhdI         1260  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          497  AGCT                 AG^CT
AspEI        1260  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI           30  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1384  GGATCC               G^GATCC
BanII         957  GGGCTC               G[GA]GC[CT]^C
BclI         1298  TGATCA               T^GATCA
BpmI          650  CTGGAG               CTGGAG(16/14)
BseRI         217  GAGGAG               GAGGAG(10/8)
BsmBI        1258  GAGACG               CGTCTC(1/5)
Bsp1407I      858  TGTACA               T^GTACA
BspMI         821  GCAGGT               ACCTGC(4/8)
BsrFI         925  GCCGGC               [GA]^CCGG[CT]
BsrGI         858  TGTACA               T^GTACA
BstBI        1427  TTCGAA               TT^CGAA
BstEII         51  GGTGACC              G^GT[ACGT]ACC
BstPI          51  GGTGACC              G^GT[ACGT]ACC
BstXI         366  CCAATCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1330  GCGC                 GCG^C
Cfr10I        925  GCCGGC               [GA]^CCGG[CT]
DraII         791  AGGTCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I     1260  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      791  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I        51  GGTGACC              G^GT[ACGT]ACC
EcoRV         580  GATATC               GAT^ATC
FauI           25  GCGGG                CCCGC(4/6)
FbaI         1298  TGATCA               T^GATCA
HaeII        1329  GGCGCT               [GA]GCGC^[CT]
HgaI           59  GCGTC                GACGC(5/10)
HhaI         1330  GCGC                 GCG^C
HinP1I       1330  GCGC                 G^CGC
MspA1I       1403  CAGCGG               C[AC]G^C[GT]G
NaeI          925  GCCGGC               GCC^GGC
NgoMIV        925  GCCGGC               G^CCGGC
NspV         1427  TTCGAA               TT^CGAA
PflFI          30  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         791  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          893  CTGCAG               CTGCA^G
SalI         1268  GTCGAC               G^TCGAC
SfuI         1427  TTCGAA               TT^CGAA
SmaI           77  CCCGGG               CCC^GGG
SspBI         858  TGTACA               T^GTACA
StuI          344  AGGCCT               AGG^CCT
TfiI          760  GATTC                G^A[AT]TC
Tth111I        30  GACGTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI          77  CCCGGG               C^CCGGG
XmaI           77  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AciI           25  GCGG                 CCGC(-3/-1)
             1405  GCGG                
Alw44I        445  GTGCAC               G^TGCAC
              995  GTGCAC              
AlwNI         862  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              978  CAGCATCTG           
AocI          453  CCTCAGG              CC^T[ACGT]AGG
              940  CCTGAGG             
ApaLI         445  GTGCAC               G^TGCAC
              995  GTGCAC              
AseI          742  ATTAAT               AT^TAAT
             1105  ATTAAT              
AsnI          742  ATTAAT               AT^TAAT
             1105  ATTAAT              
AvaI           77  CCCGGG               C^[CT]CG[GA]G
              242  CTCGGG              
AvaII         437  GGTCC                G^G[AT]CC
              792  GGTCC               
BcnI           77  CCCGG                CC^[GC]GG
               78  CCGGG               
BsoBI          77  CCCGGG               C^[CT]CG[GA]G
              242  CTCGGG              
Bst1107I      180  GTATAC               GTA^TAC
              935  GTATAC              
BstF5I       1392  GGATG                GGATG(2/0)
             1407  GGATG               
BstYI         221  AGATCC               [GA]^GATC[CT]
             1384  GGATCC              
BstZ17I       180  GTATAC               GTA^TAC
              935  GTATAC              
Bsu36I        453  CCTCAGG              CC^T[ACGT]AGG
              940  CCTGAGG             
DsaI         1208  CCATGG               C^C[GA][CT]GG
             1388  CCATGG              
Eco81I        453  CCTCAGG              CC^T[ACGT]AGG
              940  CCTGAGG             
EcoT14I      1208  CCATGG               C^C[AT][AT]GG
             1388  CCATGG              
FokI         1392  GGATG                GGATG(9/13)
             1407  GGATG               
HapII          78  CCGG                 C^CGG
              926  CCGG                
HincII        517  GTTGAC               GT[CT]^[GA]AC
             1268  GTCGAC              
HindII        517  GTTGAC               GT[CT]^[GA]AC
             1268  GTCGAC              
HpaII          78  CCGG                 C^CGG
              926  CCGG                
MflI          221  AGATCC               [GA]^GATC[CT]
             1384  GGATCC              
MslI         1002  CATCATTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1320  CATCATCATG          
MspI           78  CCGG                 C^CGG
              926  CCGG                
MwoI            5  GCCGATGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              165  GCCCCCTCTGC         
NciI           77  CCCGG                CC^[GC]GG
               78  CCGGG               
NcoI         1208  CCATGG               C^CATGG
             1388  CCATGG              
NspI         1064  GCATGC               [GA]CATG^[CT]
             1369  GCATGC              
PshAI          54  GACCAGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1271  GACTGAAGTC          
PshBI         742  ATTAAT               AT^TAAT
             1105  ATTAAT              
SfaNI           8  GATGC                GCATC(5/9)
              980  GCATC               
SphI         1064  GCATGC               GCATG^C
             1369  GCATGC              
StyI         1208  CCATGG               C^C[AT][AT]GG
             1388  CCATGG              
XhoII         221  AGATCC               [GA]^GATC[CT]
             1384  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AccI        AfaI        AlwI        AspHI       BbsI        
BbvI        BmyI        BpuAI       BsaI        BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
HaeIII      HinfI       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfcI        
SspI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     
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