Restriction map for pF1KB3717
 (Linear) (Six-base) MAPSORT of: KIBB3717.seq  Check: 19  from: 1  to: 1434



KIBB3717.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AccI GT'mk_AC

Cuts at:      0      98     181     936    1269    1434 
   Size:         98      83     755     333     165

  Fragments arranged by size: 

                 755     333     165      98      83
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1294    1434 
   Size:       1294     140
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1265    1434 
   Size:       1265     169
 
AlwNI CAG_nnn'CTG

Cuts at:      0     867     983    1434 
   Size:        867     116     451

  Fragments arranged by size: 

                 867     451     116
 
ApaLI G'TGCA_C

Cuts at:      0     445     995    1434 
   Size:        445     550     439

  Fragments arranged by size: 

                 550     445     439
 
AseI AT'TA_AT

Cuts at:      0     743    1106    1434 
   Size:        743     363     328

  Fragments arranged by size: 

                 743     363     328
 
AvaI C'yCGr_G

Cuts at:      0      77     242    1434 
   Size:         77     165    1192

  Fragments arranged by size: 

                1192     165      77
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      90     123    1434 
   Size:         90      33    1311

  Fragments arranged by size: 

                1311      90      33
 
BamHI G'GATC_C

Cuts at:      0    1384    1434 
   Size:       1384      50
 
BanII G_rGCy'C

Cuts at:      0     961    1434 
   Size:        961     473
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      75     662     795    1088    1434 
   Size:         75     587     133     293     346

  Fragments arranged by size: 

                 587     346     293     133      75
 
BclI T'GATC_A

Cuts at:      0    1298    1434 
   Size:       1298     136
 
Bme1580I G_kGCm'C

Cuts at:      0     449     999    1434 
   Size:        449     550     435

  Fragments arranged by size: 

                 550     449     435
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1213    1434 
   Size:       1213     221
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671    1434 
   Size:        671     763
 
Bpu10I CC'TnA_GC

Cuts at:      0     960    1434 
   Size:        960     474
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     229     356     371    1434 
   Size:        229     127      15    1063

  Fragments arranged by size: 

                1063     229     127      15
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     623     653    1434 
   Size:        623      30     781

  Fragments arranged by size: 

                 781     623      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     232    1434 
   Size:        232    1202
 
BseYI C'CCAG_C

Cuts at:      0    1332    1434 
   Size:       1332     102
 
BsiHKAI G_wGCw'C

Cuts at:      0     449     999    1419    1434 
   Size:        449     550     420      15

  Fragments arranged by size: 

                 550     449     420      15
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1252    1434 
   Size:       1252     182
 
Bsp1286I G_dGCh'C

Cuts at:      0     449     961     999    1419    1434 
   Size:        449     512      38     420      15

  Fragments arranged by size: 

                 512     449     420      38      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     812    1434 
   Size:        812     622
 
BsrFI r'CCGG_y

Cuts at:      0     925    1434 
   Size:        925     509
 
BsrGI T'GTAC_A

Cuts at:      0     858    1434 
   Size:        858     576
 
BstBI TT'CG_AA

Cuts at:      0    1428    1434 
   Size:       1428       6
 
BstEII G'GTnAC_C

Cuts at:      0      51    1434 
   Size:         51    1383
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     373    1434 
   Size:        373    1061
 
BstYI r'GATC_y

Cuts at:      0     221    1384    1434 
   Size:        221    1163      50

  Fragments arranged by size: 

                1163     221      50
 
BstZ17I GTA'TAC

Cuts at:      0     182     937    1434 
   Size:        182     755     497

  Fragments arranged by size: 

                 755     497     182
 
Bsu36I CC'TnA_GG

Cuts at:      0     454     941    1434 
   Size:        454     487     493

  Fragments arranged by size: 

                 493     487     454
 
BtgI C'CryG_G

Cuts at:      0    1208    1388    1434 
   Size:       1208     180      46

  Fragments arranged by size: 

                1208     180      46
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     665     700    1434 
   Size:        665      35     734

  Fragments arranged by size: 

                 734     665      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     671    1294    1434 
   Size:        671     623     140

  Fragments arranged by size: 

                 671     623     140
 
EcoO109I rG'GnC_Cy

Cuts at:      0     792    1434 
   Size:        792     642
 
EcoRV GAT'ATC

Cuts at:      0     582    1434 
   Size:        582     852
 
HaeII r_GCGC'y

Cuts at:      0    1333    1434 
   Size:       1333     101
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1251    1283    1434 
   Size:       1251      32     151

  Fragments arranged by size: 

                1251     151      32
 
HincII GTy'rAC

Cuts at:      0     519    1270    1434 
   Size:        519     751     164

  Fragments arranged by size: 

                 751     519     164
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     354    1434 
   Size:        354    1080
 
MslI CAynn'nnrTG

Cuts at:      0    1006    1324    1434 
   Size:       1006     318     110

  Fragments arranged by size: 

                1006     318     110
 
MspA1I CmG'CkG

Cuts at:      0    1405    1434 
   Size:       1405      29
 
NaeI GCC'GGC

Cuts at:      0     927    1434 
   Size:        927     507
 
NcoI C'CATG_G

Cuts at:      0    1208    1388    1434 
   Size:       1208     180      46

  Fragments arranged by size: 

                1208     180      46
 
NgoMIV G'CCGG_C

Cuts at:      0     925    1434 
   Size:        925     509
 
NspI r_CATG'y

Cuts at:      0    1068    1373    1434 
   Size:       1068     305      61

  Fragments arranged by size: 

                1068     305      61
 
PpuMI rG'GwC_Cy

Cuts at:      0     792    1434 
   Size:        792     642
 
PshAI GACnn'nnGTC

Cuts at:      0      58    1275    1434 
   Size:         58    1217     159

  Fragments arranged by size: 

                1217     159      58
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     172     204    1434 
   Size:        172      32    1230

  Fragments arranged by size: 

                1230     172      32
 
PstI C_TGCA'G

Cuts at:      0     897    1434 
   Size:        897     537
 
SalI G'TCGA_C

Cuts at:      0    1268    1434 
   Size:       1268     166
 
SfcI C'TryA_G

Cuts at:      0      95     270     893    1434 
   Size:         95     175     623     541

  Fragments arranged by size: 

                 623     541     175      95
 
SmaI CCC'GGG

Cuts at:      0      79    1434 
   Size:         79    1355
 
SphI G_CATG'C

Cuts at:      0    1068    1373    1434 
   Size:       1068     305      61

  Fragments arranged by size: 

                1068     305      61
 
SspI AAT'ATT

Cuts at:      0     297     411     902    1434 
   Size:        297     114     491     532

  Fragments arranged by size: 

                 532     491     297     114
 
StuI AGG'CCT

Cuts at:      0     346    1434 
   Size:        346    1088
 
StyI C'CwwG_G

Cuts at:      0    1208    1388    1434 
   Size:       1208     180      46

  Fragments arranged by size: 

                1208     180      46
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     785    1014    1085    1324    1434 
   Size:        785     229      71     239     110

  Fragments arranged by size: 

                 785     239     229     110      71
 
TatI w'GTAC_w

Cuts at:      0     858    1434 
   Size:        858     576
 
Tth111I GACn'n_nGTC

Cuts at:      0      33    1434 
   Size:         33    1401
 
XmaI C'CCGG_G

Cuts at:      0      77    1434 
   Size:         77    1357
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI    ApaLI     AseI     AvaI     BaeI
    BamHI    BanII     BbsI     BclI Bme1580I     BmrI     BpmI   Bpu10I
     BsaI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrFI    BsrGI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    CspCI  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I   HincII
     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI    PpuMI
    PshAI     PsrI     PstI     SalI     SfcI     SmaI     SphI     SspI
     StuI     StyI    TaqII     TatI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI     ApoI     AscI    AsiSI    AvrII
     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI
    BsrDI   BssHII    BssSI   BstAPI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     SwaI
     TstI     XbaI     XcmI     XhoI     XmnI     ZraI
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