Restriction map for pF1KB3699
 (Linear) (Six-base) MAPSORT of: KIBB3699.seq  Check: 9879  from: 1  to: 2553



KIBB3699.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AclI AA'CG_TT

Cuts at:      0    1224    2553 
   Size:       1224    1329
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     847     856    1345    1385    1420    1723    1788    1789 
   Size:        847       9     489      40      35     303      65       1

Cuts at:   1789    2242    2400    2553 
   Size:        453     158     153

  Fragments arranged by size: 

                 847     489     453     303     158     153      65      40
                  35       9       1
 
AflII C'TTAA_G

Cuts at:      0    2134    2553 
   Size:       2134     419
 
AhdI GACnn_n'nnGTC

Cuts at:      0     234    2553 
   Size:        234    2319
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     155    2553 
   Size:        155    2398
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     380     412     766     798    2553 
   Size:        380      32     354      32    1755

  Fragments arranged by size: 

                1755     380     354      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     825     930    1323    1335    2423    2553 
   Size:        825     105     393      12    1088     130

  Fragments arranged by size: 

                1088     825     393     130     105      12
 
ApaI G_GGCC'C

Cuts at:      0     375    1492    2553 
   Size:        375    1117    1061

  Fragments arranged by size: 

                1117    1061     375
 
ApoI r'AATT_y

Cuts at:      0     265     350    1676    2553 
   Size:        265      85    1326     877

  Fragments arranged by size: 

                1326     877     265      85
 
AvaI C'yCGr_G

Cuts at:      0    1162    2006    2553 
   Size:       1162     844     547

  Fragments arranged by size: 

                1162     844     547
 
BamHI G'GATC_C

Cuts at:      0     297     443    1596    2553 
   Size:        297     146    1153     957

  Fragments arranged by size: 

                1153     957     297     146
 
BanI G'GyrC_C

Cuts at:      0    1786    2181    2553 
   Size:       1786     395     372

  Fragments arranged by size: 

                1786     395     372
 
BanII G_rGCy'C

Cuts at:      0     375     747    1492    1639    2198    2294    2553 
   Size:        375     372     745     147     559      96     259

  Fragments arranged by size: 

                 745     559     375     372     259     147      96
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     125     507     556    1780    2553 
   Size:        125     382      49    1224     773

  Fragments arranged by size: 

                1224     773     382     125      49
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1884    1918    2553 
   Size:       1884      34     635

  Fragments arranged by size: 

                1884     635      34
 
BfrBI ATG'CAT

Cuts at:      0    2303    2553 
   Size:       2303     250
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1643    2553 
   Size:       1643     910
 
BlpI GC'TnA_GC

Cuts at:      0    1423    1549    2197    2553 
   Size:       1423     126     648     356

  Fragments arranged by size: 

                1423     648     356     126
 
Bme1580I G_kGCm'C

Cuts at:      0     375    1492    2553 
   Size:        375    1117    1061

  Fragments arranged by size: 

                1117    1061     375
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     300    2553 
   Size:        300    2253
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793     812    1033    1497    1702    2035    2149    2173 
   Size:        793      19     221     464     205     333     114      24

Cuts at:   2173    2284    2296    2553 
   Size:        111      12     257

  Fragments arranged by size: 

                 793     464     333     257     221     205     114     111
                  24      19      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     985    2553 
   Size:        985    1568
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2097    2553 
   Size:       2097     456
 
BsaWI w'CCGG_w

Cuts at:      0    1118    2553 
   Size:       1118    1435
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     380     410     766     796    2553 
   Size:        380      30     356      30    1757

  Fragments arranged by size: 

                1757     380     356      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     721    1091    1503    1972    2553 
   Size:        721     370     412     469     581

  Fragments arranged by size: 

                 721     581     469     412     370
 
BseYI C'CCAG_C

Cuts at:      0     719     959    1485    2509    2553 
   Size:        719     240     526    1024      44

  Fragments arranged by size: 

                1024     719     526     240      44
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     125    1267    2553 
   Size:         37      88    1142    1286

  Fragments arranged by size: 

                1286    1142      88      37
 
BsiHKAI G_wGCw'C

Cuts at:      0     747    1850    1871    2198    2553 
   Size:        747    1103      21     327     355

  Fragments arranged by size: 

                1103     747     355     327      21
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2505    2553 
   Size:       2505      48
 
Bsp1286I G_dGCh'C

Cuts at:      0     375     747    1492    1639    1850    1871    2198    2294 
   Size:        375     372     745     147     211      21     327      96

Cuts at:   2294    2553 
   Size:        259

  Fragments arranged by size: 

                 745     375     372     327     259     211     147      96
                  21
 
BspEI T'CCGG_A

Cuts at:      0    1118    2553 
   Size:       1118    1435
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1840    2553 
   Size:       1840     713
 
BsrDI GCAATG_nn'

Cuts at:      0    1065    2553 
   Size:       1065    1488
 
BsrFI r'CCGG_y

Cuts at:      0    1760    2553 
   Size:       1760     793
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     258    1832    2553 
   Size:        258    1574     721

  Fragments arranged by size: 

                1574     721     258
 
BstEII G'GTnAC_C

Cuts at:      0    1201    2455    2553 
   Size:       1201    1254      98

  Fragments arranged by size: 

                1254    1201      98
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     888    2553 
   Size:        888    1665
 
BstYI r'GATC_y

Cuts at:      0     297     443     533    1596    1707    2235    2553 
   Size:        297     146      90    1063     111     528     318

  Fragments arranged by size: 

                1063     528     318     297     146     111      90
 
Bsu36I CC'TnA_GG

Cuts at:      0     814    2553 
   Size:        814    1739
 
BtgI C'CryG_G

Cuts at:      0     756    2483    2553 
   Size:        756    1727      70

  Fragments arranged by size: 

                1727     756      70
 
BtsI GCAGTG_nn'

Cuts at:      0      13     655    1978    2215    2553 
   Size:         13     642    1323     237     338

  Fragments arranged by size: 

                1323     642     338     237      13
 
DraIII CAC_nnn'GTG

Cuts at:      0    1267    2553 
   Size:       1267    1286
 
EaeI y'GGCC_r

Cuts at:      0     213    1524    2472    2553 
   Size:        213    1311     948      81

  Fragments arranged by size: 

                1311     948     213      81
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1924    2553 
   Size:       1924     629
 
EcoICRI GAG'CTC

Cuts at:      0     745    2196    2553 
   Size:        745    1451     357

  Fragments arranged by size: 

                1451     745     357
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793     812     847     856    1033    1345    1385    1420 
   Size:        793      19      35       9     177     312      40      35

Cuts at:   1420    1497    1702    1723    1788    1789    2035    2149    2173 
   Size:         77     205      21      65       1     246     114      24

Cuts at:   2173    2242    2284    2296    2400    2553 
   Size:         69      42      12     104     153

  Fragments arranged by size: 

                 793     312     246     205     177     153     114     104
                  77      69      65      42      40      35      35      24
                  21      19      12       9       1
 
EcoO109I rG'GnC_Cy

Cuts at:      0     228     371    1470    1489    2338    2553 
   Size:        228     143    1099      19     849     215

  Fragments arranged by size: 

                1099     849     228     215     143      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     160    2553 
   Size:        160    2393
 
HaeII r_GCGC'y

Cuts at:      0    1761    2553 
   Size:       1761     792
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     220     252     348     380     421     453     518     543 
   Size:        220      32      96      32      41      32      65      25

Cuts at:    543     550     575     980    1012    1581    1613    2140    2172 
   Size:          7      25     405      32     569      32     527      32

Cuts at:   2172    2239    2271    2553 
   Size:         67      32     282

  Fragments arranged by size: 

                 569     527     405     282     220      96      67      65
                  41      32      32      32      32      32      32      32
                  25      25       7
 
HindIII A'AGCT_T

Cuts at:      0    2015    2553 
   Size:       2015     538
 
MfeI C'AATT_G

Cuts at:      0     376    1388    2553 
   Size:        376    1012    1165

  Fragments arranged by size: 

                1165    1012     376
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     683     743    1394    1481    1726    2553 
   Size:        683      60     651      87     245     827

  Fragments arranged by size: 

                 827     683     651     245      87      60
 
MscI TGG'CCA

Cuts at:      0    2474    2553 
   Size:       2474      79
 
MslI CAynn'nnrTG

Cuts at:      0    1853    2553 
   Size:       1853     700
 
MspA1I CmG'CkG

Cuts at:      0      51     198     771     790     822    1548    1920    2127 
   Size:         51     147     573      19      32     726     372     207

Cuts at:   2127    2553 
   Size:        426

  Fragments arranged by size: 

                 726     573     426     372     207     147      51      32
                  19
 
NaeI GCC'GGC

Cuts at:      0    1762    2553 
   Size:       1762     791
 
NgoMIV G'CCGG_C

Cuts at:      0    1760    2553 
   Size:       1760     793
 
NsiI A_TGCA'T

Cuts at:      0    2305    2553 
   Size:       2305     248
 
NspI r_CATG'y

Cuts at:      0     972    1557    2553 
   Size:        972     585     996

  Fragments arranged by size: 

                 996     972     585
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     189     762    2553 
   Size:        189     573    1791

  Fragments arranged by size: 

                1791     573     189
 
PfoI T'CCnGG_A

Cuts at:      0     439     446    1049    2051    2553 
   Size:        439       7     603    1002     502

  Fragments arranged by size: 

                1002     603     502     439       7
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     380     412     766     798    1369    1401    2553 
   Size:        380      32     354      32     571      32    1152

  Fragments arranged by size: 

                1152     571     380     354      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     228    2338    2553 
   Size:        228    2110     215

  Fragments arranged by size: 

                2110     228     215
 
PspOMI G'GGCC_C

Cuts at:      0     371    1488    2553 
   Size:        371    1117    1065

  Fragments arranged by size: 

                1117    1065     371
 
PstI C_TGCA'G

Cuts at:      0      23     251     599    1910    2553 
   Size:         23     228     348    1311     643

  Fragments arranged by size: 

                1311     643     348     228      23
 
PvuII CAG'CTG

Cuts at:      0      51     198     771     790     822    1548    2553 
   Size:         51     147     573      19      32     726    1005

  Fragments arranged by size: 

                1005     726     573     147      51      32      19
 
SacI G_AGCT'C

Cuts at:      0     747    2198    2553 
   Size:        747    1451     355

  Fragments arranged by size: 

                1451     747     355
 
ScaI AGT'ACT

Cuts at:      0     100    2553 
   Size:        100    2453
 
SfcI C'TryA_G

Cuts at:      0      19     247     595     922    1584    1906    2553 
   Size:         19     228     348     327     662     322     647

  Fragments arranged by size: 

                 662     647     348     327     322     228      19
 
SmaI CCC'GGG

Cuts at:      0    2008    2553 
   Size:       2008     545
 
SmlI C'TyrA_G

Cuts at:      0    1000    2134    2553 
   Size:       1000    1134     419

  Fragments arranged by size: 

                1134    1000     419
 
SphI G_CATG'C

Cuts at:      0     972    1557    2553 
   Size:        972     585     996

  Fragments arranged by size: 

                 996     972     585
 
StuI AGG'CCT

Cuts at:      0    1532    2553 
   Size:       1532    1021
 
StyI C'CwwG_G

Cuts at:      0     161    1466    2334    2553 
   Size:        161    1305     868     219

  Fragments arranged by size: 

                1305     868     219     161
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1830    2012    2553 
   Size:       1830     182     541

  Fragments arranged by size: 

                1830     541     182
 
TatI w'GTAC_w

Cuts at:      0      98    2553 
   Size:         98    2455
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1970    2002    2553 
   Size:       1970      32     551

  Fragments arranged by size: 

                1970     551      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1779    2553 
   Size:       1779     774
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1607    1661    2301    2553 
   Size:       1607      54     640     252

  Fragments arranged by size: 

                1607     640     252      54
 
XmaI C'CCGG_G

Cuts at:      0    2006    2553 
   Size:       2006     547
 

 Enzymes that do cut:

     AclI     AcuI    AflII     AhdI     AlfI     AloI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BfrBI
     BglI     BlpI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     EaeI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NaeI   NgoMIV     NsiI     NspI    PflMI     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII     SacI     ScaI     SfcI     SmaI
     SmlI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI   AflIII     AgeI     AleI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI
    BciVI     BclI    BglII    BmgBI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BspHI    BsrBI    BsrGI
   BssHII    BssSI    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EarI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MluI     NarI     NcoI
     NdeI     NheI     NotI     NruI     PacI     PasI     PciI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI     XhoI
     XmnI     ZraI
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