Restriction map for
pF1KB3690
(Circular) (Six-base) MAPSORT of: pF1KB3690.seq Check: 5673 from: 1 to: 4385
pF1KB3690 4385 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 1043 1423 1043
Size: 380 4005
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 180 4225 180
Size: 4045 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 847 1220 2210 2642 2995 3657 622
Size: 225 373 990 432 353 662 1350
Fragments arranged by size:
1350 990 662 432 373 353 225
AflIII A'CryG_T
Cuts at: 1737 3537 3818 1737
Size: 1800 281 2304
Fragments arranged by size:
2304 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 732 732
Size: 4385
AlwNI CAG_nnn'CTG
Cuts at: 192 958 1320 1690 3128 192
Size: 766 362 370 1438 1449
Fragments arranged by size:
1449 1438 766 370 362
ApaI G_GGCC'C
Cuts at: 216 216
Size: 4385
ApaLI G'TGCA_C
Cuts at: 636 3223 636
Size: 2587 1798
ApoI r'AATT_y
Cuts at: 406 916 1115 1389 3738 3994 406
Size: 510 199 274 2349 256 797
Fragments arranged by size:
2349 797 510 274 256 199
AseI AT'TA_AT
Cuts at: 20 436 20
Size: 416 3969
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 783 1405 783
Size: 622 3763
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 886 919 886
Size: 33 4352
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 684 1401 1815 2063 2098 684
Size: 717 414 248 35 2971
Fragments arranged by size:
2971 717 414 248 35
BanII G_rGCy'C
Cuts at: 216 1399 2429 216
Size: 1183 1030 2172
Fragments arranged by size:
2172 1183 1030
BbeI G_GCGC'C
Cuts at: 688 1819 2067 688
Size: 1131 248 3006
Fragments arranged by size:
3006 1131 248
BbsI GAAGACnn'nnnn_
Cuts at: 1268 1268
Size: 4385
BciVI GTATCCnnnnn_n'
Cuts at: 914 2283 3339 4385 914
Size: 1369 1056 1046 914
Fragments arranged by size:
1369 1056 1046 914
BclI T'GATC_A
Cuts at: 801 1905 801
Size: 1104 3281
BglII A'GATC_T
Cuts at: 993 1900 3937 993
Size: 907 2037 1441
Fragments arranged by size:
2037 1441 907
BlpI GC'TnA_GC
Cuts at: 1497 1497
Size: 4385
Bme1580I G_kGCm'C
Cuts at: 216 640 2010 2103 3227 216
Size: 424 1370 93 1124 1374
Fragments arranged by size:
1374 1370 1124 424 93
BmrI ACTGGGnnnn_n'
Cuts at: 1122 1597 1678 2013 4157 1122
Size: 475 81 335 2144 1350
Fragments arranged by size:
2144 1350 475 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2440 3154 3452 1561
Size: 879 714 298 2494
Fragments arranged by size:
2494 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 663 1738 663
Size: 1075 3310
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 685 1816 2064 2766 4262 685
Size: 1131 248 702 1496 808
Fragments arranged by size:
1496 1131 808 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3184 3331 3605 1574
Size: 521 678 411 147 274 2354
Fragments arranged by size:
2354 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 828 1810 2568 3233 3641 828
Size: 982 758 665 408 1572
Fragments arranged by size:
1572 982 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 579
Size: 4385
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3203 104
Size: 1823 46 1230 1286
Fragments arranged by size:
1823 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 640 1399 2177 2367 3227 640
Size: 759 778 190 860 1798
Fragments arranged by size:
1798 860 778 759 190
BsmI GAATG_Cn'
Cuts at: 534 534
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 216 640 1399 2010 2103 2177 2367 2429 3227
Size: 424 759 611 93 74 190 62 798
Cuts at: 3227 216
Size: 1374
Fragments arranged by size:
1374 798 759 611 424 190 93 74
62
BspHI T'CATG_A
Cuts at: 480 647 851 2817 480
Size: 167 204 1966 2048
Fragments arranged by size:
2048 1966 204 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1435 1649 1951 2332 1435
Size: 214 302 381 3488
Fragments arranged by size:
3488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2677 2731 4215 4380 50
Size: 2627 54 1484 165 55
Fragments arranged by size:
2627 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2297 2297
Size: 4385
BsrFI r'CCGG_y
Cuts at: 2383 2564 2383
Size: 181 4204
BssSI C'ACGA_G
Cuts at: 2656 3364 2656
Size: 708 3677
BstAPI GCAn_nnn'nTGC
Cuts at: 1762 1762
Size: 4385
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstYI r'GATC_y
Cuts at: 1 605 993 1034 1410 1877 1900 2235 2481
Size: 604 388 41 376 467 23 335 246
Cuts at: 2481 2787 2799 2885 3937 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1052 604 467 449 388 376 335 306
246 86 41 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 1628 1628
Size: 4385
ClaI AT'CG_AT
Cuts at: 1282 1282
Size: 4385
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 2091 3435 2091
Size: 1344 3041
EaeI y'GGCC_r
Cuts at: 1970 2144 2535 2562 3907 1970
Size: 174 391 27 1345 2448
Fragments arranged by size:
2448 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 783 2408 2618 783
Size: 1625 210 2550
Fragments arranged by size:
2550 1625 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 3335 3481 4195 2593
Size: 742 146 714 2783
Fragments arranged by size:
2783 742 714 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 847 1220 2210 2642 2995 3657 622
Size: 225 373 990 432 353 662 1350
Fragments arranged by size:
1350 990 662 432 373 353 225
EcoO109I rG'GnC_Cy
Cuts at: 212 1360 1524 212
Size: 1148 164 3073
Fragments arranged by size:
3073 1148 164
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 302 2404 302
Size: 2102 2283
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 688 1819 2067 3297 4068 688
Size: 1131 248 1230 771 1005
Fragments arranged by size:
1230 1131 1005 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 303 335 718 750 985 1017 1282 1314 2227
Size: 32 383 32 235 32 265 32 913
Cuts at: 2227 2257 2259 2289 303
Size: 30 2 30 2399
Fragments arranged by size:
2399 913 383 265 235 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 684 1815 2063 684
Size: 1131 248 3006
Fragments arranged by size:
3006 1131 248
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MfeI C'AATT_G
Cuts at: 200 1151 200
Size: 951 3434
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1112 3144 3328 1112
Size: 2032 184 2169
Fragments arranged by size:
2169 2032 184
MscI TGG'CCA
Cuts at: 2146 2146
Size: 4385
MslI CAynn'nnrTG
Cuts at: 580 2374 2501 580
Size: 1794 127 2464
Fragments arranged by size:
2464 1794 127
MspA1I CmG'CkG
Cuts at: 192 541 828 1496 1810 2170 2954 3199 192
Size: 349 287 668 314 360 784 245 1378
Fragments arranged by size:
1378 784 668 360 349 314 287 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 685 1816 2064 685
Size: 1131 248 3006
Fragments arranged by size:
3006 1131 248
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 1150 1438 1938 3541 1150
Size: 288 500 1603 1994
Fragments arranged by size:
1994 1603 500 288
PciI A'CATG_T
Cuts at: 3537 3537
Size: 4385
PfoI T'CCnGG_A
Cuts at: 906 906
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 317 349 2831 2863 317
Size: 32 2482 32 1839
Fragments arranged by size:
2482 1839 32 32
PpuMI rG'GwC_Cy
Cuts at: 1360 1360
Size: 4385
PshAI GACnn'nnGTC
Cuts at: 1340 1340
Size: 4385
PspOMI G'GGCC_C
Cuts at: 212 212
Size: 4385
PstI C_TGCA'G
Cuts at: 1432 2117 1432
Size: 685 3700
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 192 541 828 1810 2170 192
Size: 349 287 982 360 2407
Fragments arranged by size:
2407 982 360 349 287
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SanDI GG'GwC_CC
Cuts at: 1360 1360
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 2408 2618 2408
Size: 210 4175
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 1040 1428 2113 3081 3272 33
Size: 1007 388 685 968 191 1146
Fragments arranged by size:
1146 1007 968 685 388 191
SfoI GGC'GCC
Cuts at: 686 1817 2065 686
Size: 1131 248 3006
Fragments arranged by size:
3006 1131 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 785 1407 785
Size: 622 3763
SmlI C'TyrA_G
Cuts at: 1540 2455 3169 3431 1540
Size: 915 714 262 2494
Fragments arranged by size:
2494 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 1438 1938 1438
Size: 500 3885
StyI C'CwwG_G
Cuts at: 216 1519 1873 216
Size: 1303 354 2728
Fragments arranged by size:
2728 1303 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1076 1968 2101 2560 2769 1076
Size: 892 133 459 209 2692
Fragments arranged by size:
2692 892 459 209 133
TatI w'GTAC_w
Cuts at: 3900 3900
Size: 4385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 213 245 3902 3934 213
Size: 32 3657 32 664
Fragments arranged by size:
3657 664 32 32
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 399 1416 399
Size: 1017 3368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 514 1880 514
Size: 1366 3019
XmaI C'CCGG_G
Cuts at: 783 1405 783
Size: 622 3763
XmnI GAAnn'nnTTC
Cuts at: 407 929 407
Size: 522 3863
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmBI BspEI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI