Restriction map for pF1KB3690
 (Linear) (Six-base) MAPSORT of: KIBB3690.seq  Check: 9387  from: 1  to: 1272



KIBB3690.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AccI GT'mk_AC

Cuts at:      0     935    1272 
   Size:        935     337
 
AclI AA'CG_TT

Cuts at:      0      72    1272 
   Size:         72    1200
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     739    1112    1272 
   Size:        514     225     373     160

  Fragments arranged by size: 

                 514     373     225     160
 
AhdI GACnn_n'nnGTC

Cuts at:      0     624    1272 
   Size:        624     648
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84     850    1212    1272 
   Size:         84     766     362      60

  Fragments arranged by size: 

                 766     362      84      60
 
ApaI G_GGCC'C

Cuts at:      0     108    1272 
   Size:        108    1164
 
ApaLI G'TGCA_C

Cuts at:      0     528    1272 
   Size:        528     744
 
ApoI r'AATT_y

Cuts at:      0     298     808    1007    1272 
   Size:        298     510     199     265

  Fragments arranged by size: 

                 510     298     265     199
 
AseI AT'TA_AT

Cuts at:      0     328    1272 
   Size:        328     944
 
AvaI C'yCGr_G

Cuts at:      0     675    1272 
   Size:        675     597
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     778     811    1272 
   Size:        778      33     461

  Fragments arranged by size: 

                 778     461      33
 
BanI G'GyrC_C

Cuts at:      0     576    1272 
   Size:        576     696
 
BanII G_rGCy'C

Cuts at:      0     108    1272 
   Size:        108    1164
 
BbeI G_GCGC'C

Cuts at:      0     580    1272 
   Size:        580     692
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1160    1272 
   Size:       1160     112
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     806    1272 
   Size:        806     466
 
BclI T'GATC_A

Cuts at:      0     693    1272 
   Size:        693     579
 
BglII A'GATC_T

Cuts at:      0     885    1272 
   Size:        885     387
 
Bme1580I G_kGCm'C

Cuts at:      0     108     532    1272 
   Size:        108     424     740

  Fragments arranged by size: 

                 740     424     108
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1014    1272 
   Size:       1014     258
 
BsaAI yAC'GTr

Cuts at:      0     555    1272 
   Size:        555     717
 
BsaHI Gr'CG_yC

Cuts at:      0     577    1272 
   Size:        577     695
 
BseYI C'CCAG_C

Cuts at:      0     720    1272 
   Size:        720     552
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     471    1272 
   Size:        471     801
 
BsiHKAI G_wGCw'C

Cuts at:      0     532    1272 
   Size:        532     740
 
BsmI GAATG_Cn'

Cuts at:      0     426    1272 
   Size:        426     846
 
Bsp1286I G_dGCh'C

Cuts at:      0     108     532    1272 
   Size:        108     424     740

  Fragments arranged by size: 

                 740     424     108
 
BspHI T'CATG_A

Cuts at:      0     372     539     743    1272 
   Size:        372     167     204     529

  Fragments arranged by size: 

                 529     372     204     167
 
BstYI r'GATC_y

Cuts at:      0     497     885     926    1272 
   Size:        497     388      41     346

  Fragments arranged by size: 

                 497     388     346      41
 
ClaI AT'CG_AT

Cuts at:      0    1174    1272 
   Size:       1174      98
 
EarI CTCTTCn'nnn_

Cuts at:      0     675    1272 
   Size:        675     597
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     739    1112    1272 
   Size:        514     225     373     160

  Fragments arranged by size: 

                 514     373     225     160
 
EcoO109I rG'GnC_Cy

Cuts at:      0     104    1252    1272 
   Size:        104    1148      20

  Fragments arranged by size: 

                1148     104      20
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     194    1272 
   Size:        194    1078
 
HaeII r_GCGC'y

Cuts at:      0     580    1272 
   Size:        580     692
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     195     227     610     642     877     909    1174    1206 
   Size:        195      32     383      32     235      32     265      32

Cuts at:   1206    1272 
   Size:         66

  Fragments arranged by size: 

                 383     265     235     195      66      32      32      32
                  32
 
KasI G'GCGC_C

Cuts at:      0     576    1272 
   Size:        576     696
 
MfeI C'AATT_G

Cuts at:      0      92    1043    1272 
   Size:         92     951     229

  Fragments arranged by size: 

                 951     229      92
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1004    1272 
   Size:       1004     268
 
MslI CAynn'nnrTG

Cuts at:      0     472    1272 
   Size:        472     800
 
MspA1I CmG'CkG

Cuts at:      0      84     433     720    1272 
   Size:         84     349     287     552

  Fragments arranged by size: 

                 552     349     287      84
 
NarI GG'CG_CC

Cuts at:      0     577    1272 
   Size:        577     695
 
NspI r_CATG'y

Cuts at:      0    1042    1272 
   Size:       1042     230
 
PfoI T'CCnGG_A

Cuts at:      0     798    1272 
   Size:        798     474
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     209     241    1272 
   Size:        209      32    1031

  Fragments arranged by size: 

                1031     209      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1252    1272 
   Size:       1252      20
 
PshAI GACnn'nnGTC

Cuts at:      0    1232    1272 
   Size:       1232      40
 
PspOMI G'GGCC_C

Cuts at:      0     104    1272 
   Size:        104    1168
 
PvuII CAG'CTG

Cuts at:      0      84     433     720    1272 
   Size:         84     349     287     552

  Fragments arranged by size: 

                 552     349     287      84
 
SanDI GG'GwC_CC

Cuts at:      0    1252    1272 
   Size:       1252      20
 
SfcI C'TryA_G

Cuts at:      0     932    1272 
   Size:        932     340
 
SfoI GGC'GCC

Cuts at:      0     578    1272 
   Size:        578     694
 
SmaI CCC'GGG

Cuts at:      0     677    1272 
   Size:        677     595
 
StyI C'CwwG_G

Cuts at:      0     108    1272 
   Size:        108    1164
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     968    1272 
   Size:        968     304
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     105     137    1272 
   Size:        105      32    1135

  Fragments arranged by size: 

                1135     105      32
 
XbaI T'CTAG_A

Cuts at:      0     291    1272 
   Size:        291     981
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     406    1272 
   Size:        406     866
 
XmaI C'CCGG_G

Cuts at:      0     675    1272 
   Size:        675     597
 
XmnI GAAnn'nnTTC

Cuts at:      0     299     821    1272 
   Size:        299     522     451

  Fragments arranged by size: 

                 522     451     299
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AhdI    AlwNI     ApaI    ApaLI     ApoI
     AseI     AvaI     BaeI     BanI    BanII     BbeI     BbsI    BciVI
     BclI    BglII Bme1580I     BmrI    BsaAI    BsaHI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BstYI     ClaI     EarI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I     KasI     MfeI     MmeI     MslI
   MspA1I     NarI     NspI     PfoI     PpiI    PpuMI    PshAI   PspOMI
    PvuII    SanDI     SfcI     SfoI     SmaI     StyI    TaqII     TstI
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI     AscI    AsiSI    AvrII    BamHI    BbvCI     BcgI
    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MluI     MscI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PsiI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI  Tth111I     XhoI     ZraI
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