Restriction map for pF1KB3680
##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BlnI        
BsaAI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BspDI       BspEI       BspLU11I    BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     Cfr10I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcyI         2813  GACGCC               G[GA]^CG[CT]C
AocI         2152  CCTAAGG              CC^T[ACGT]AGG
AspI         2752  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          649  CCCGGG               C^[CT]CG[GA]G
BamHI        1257  GGATCC               G^GATCC
BglII        1401  AGATCT               A^GATCT
BsaHI        2813  GACGCC               G[GA]^CG[CT]C
BsaI          217  GAGACC               GGTCTC(1/5)
BsmI         2562  GAATGC               GAATGC(1/-1)
BsoBI         649  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     2573  TGTACA               T^GTACA
BspMI        1752  ACCTGC               ACCTGC(4/8)
BsrBI        1615  GAGCGG               CCGCTC(-3/-3)
BsrGI        2573  TGTACA               T^GTACA
Bsu36I       2152  CCTAAGG              CC^T[ACGT]AGG
CfoI         1159  GCGC                 GCG^C
DraII         932  GGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI         2587  GACCATGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2152  CCTAAGG              CC^T[ACGT]AGG
EcoNI         915  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      932  GGGTCCT              [GA]G^G[ACGT]CC[CT]
HgaI         2813  GACGC                GACGC(5/10)
HhaI         1159  GCGC                 GCG^C
Hin1I        2813  GACGCC               G[GA]^CG[CT]C
HinP1I       1159  GCGC                 G^CGC
NspI         1964  GCATGT               [GA]CATG^[CT]
PflFI        2752  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1975  CCATCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         932  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI          817  GAGCTC               GAGCT^C
SapI         1444  GAAGAGC              GCTCTTC(1/4)
SmaI          649  CCCGGG               CCC^GGG
SspBI        2573  TGTACA               T^GTACA
StuI         2467  AGGCCT               AGG^CCT
Tth111I      2752  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       1975  CCATCACATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1241  CCATGGAGGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         649  CCCGGG               C^CCGGG
XmaI          649  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcsI         2348  AAATTT               [GA]^AATT[CT]
             2530  GAATTT              
ApoI         2348  AAATTT               [GA]^AATT[CT]
             2530  GAATTT              
Asp700I       379  GAACTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2329  GAAGCTATTC          
AspHI         817  GAGCTC               G[AT]GC[AT]^C
             1007  GTGCTC              
BalI          891  TGGCCA               TGG^CCA
             2654  TGGCCA              
BclI         1304  TGATCA               T^GATCA
             1503  TGATCA              
BfaI          848  CTAG                 C^TAG
             2143  CTAG                
BlpI           30  GCTAAGC              GC^T[ACGT]AGC
              304  GCTGAGC             
Bpu1102I       30  GCTAAGC              GC^T[ACGT]AGC
              304  GCTGAGC             
BsaBI        1501  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2593  GATGTCTATC          
BsiHKAI       817  GAGCTC               G[AT]GC[AT]^C
             1007  GTGCTC              
BspHI          92  TCATGA               T^CATGA
             1148  TCATGA              
BstAPI       1000  GCATCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1894  GCAGATGTTGC         
BstEII        536  GGTTACC              G^GT[ACGT]ACC
              642  GGTAACC             
BstPI         536  GGTTACC              G^GT[ACGT]ACC
              642  GGTAACC             
BstXI         542  CCACTGTGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1811  CCACAAGTATGG        
CelII          30  GCTAAGC              GC^T[ACGT]AGC
              304  GCTGAGC             
EcoO65I       536  GGTTACC              G^GT[ACGT]ACC
              642  GGTAACC             
FbaI         1304  TGATCA               T^GATCA
             1503  TGATCA              
HincII       1220  GTCAAC               GT[CT]^[GA]AC
             2481  GTCAAC              
HindII       1220  GTCAAC               GT[CT]^[GA]AC
             2481  GTCAAC              
MaeI          848  CTAG                 C^TAG
             2143  CTAG                
MamI         1501  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2593  GATGTCTATC          
MluNI         891  TGGCCA               TGG^CCA
             2654  TGGCCA              
MscI          891  TGGCCA               TGG^CCA
             2654  TGGCCA              
MseI         1100  TTAA                 T^TAA
             2489  TTAA                
PvuII        1874  CAGCTG               CAG^CTG
             2525  CAGCTG              
RcaI           92  TCATGA               T^CATGA
             1148  TCATGA              
ScaI          200  AGTACT               AGT^ACT
             1867  AGTACT              
TaqI         1335  TCGA                 T^CGA
             2709  TCGA                
Tru9I        1100  TTAA                 T^TAA
             2489  TTAA                
TthHB8I      1335  TCGA                 T^CGA
             2709  TCGA                
XmnI          379  GAACTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2329  GAAGCTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanII       BbsI        BbvI        BcnI        
BmyI        BpmI        BpuAI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EaeI        EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
XhoII       
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