Restriction map for pF1KB3680
 (Linear) (Six-base) MAPSORT of: KIBB3680.seq  Check: 8665  from: 1  to: 2853



KIBB3680.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488    1532    2214    2726    2853 
   Size:        488    1044     682     512     127

  Fragments arranged by size: 

                1044     682     512     488     127
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     856    2853 
   Size:        856    1997
 
AlwNI CAG_nnn'CTG

Cuts at:      0     154     493     715    1581    2853 
   Size:        154     339     222     866    1272

  Fragments arranged by size: 

                1272     866     339     222     154
 
ApoI r'AATT_y

Cuts at:      0    2348    2530    2853 
   Size:       2348     182     323

  Fragments arranged by size: 

                2348     323     182
 
AvaI C'yCGr_G

Cuts at:      0     649    2853 
   Size:        649    2204
 
BamHI G'GATC_C

Cuts at:      0    1257    2853 
   Size:       1257    1596
 
BanII G_rGCy'C

Cuts at:      0     821    1730    2770    2853 
   Size:        821     909    1040      83

  Fragments arranged by size: 

                1040     909     821      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1948    2403    2698    2853 
   Size:       1948     455     295     155

  Fragments arranged by size: 

                1948     455     295     155
 
BclI T'GATC_A

Cuts at:      0    1304    1503    2853 
   Size:       1304     199    1350

  Fragments arranged by size: 

                1350    1304     199
 
BglII A'GATC_T

Cuts at:      0    1401    2853 
   Size:       1401    1452
 
BlpI GC'TnA_GC

Cuts at:      0      31     305    2853 
   Size:         31     274    2548

  Fragments arranged by size: 

                2548     274      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     192     664     736    1724    1870    2853 
   Size:        192     472      72     988     146     983

  Fragments arranged by size: 

                 988     983     472     192     146      72
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     651    2853 
   Size:        651    2202
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     692     940    1079    1196    1817    1898    2853 
   Size:        149     543     248     139     117     621      81     955

  Fragments arranged by size: 

                 955     621     543     248     149     139     117      81
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     211    2853 
   Size:        211    2642
 
BsaBI GATnn'nnATC

Cuts at:      0    1505    2597    2853 
   Size:       1505    1092     256

  Fragments arranged by size: 

                1505    1092     256
 
BsaHI Gr'CG_yC

Cuts at:      0    2814    2853 
   Size:       2814      39
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     248     278     690     720    1871    1901    2853 
   Size:        248      30     412      30    1151      30     952

  Fragments arranged by size: 

                1151     952     412     248      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     475    2125    2161    2853 
   Size:        475    1650      36     692

  Fragments arranged by size: 

                1650     692     475      36
 
BseYI C'CCAG_C

Cuts at:      0    1375    2281    2853 
   Size:       1375     906     572

  Fragments arranged by size: 

                1375     906     572
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     701    1625    1754    2482    2853 
   Size:        701     924     129     728     371

  Fragments arranged by size: 

                 924     728     701     371     129
 
BsiHKAI G_wGCw'C

Cuts at:      0     821    1011    2853 
   Size:        821     190    1842

  Fragments arranged by size: 

                1842     821     190
 
BsmI GAATG_Cn'

Cuts at:      0    2568    2853 
   Size:       2568     285
 
Bsp1286I G_dGCh'C

Cuts at:      0     821    1011    1730    2770    2853 
   Size:        821     190     719    1040      83

  Fragments arranged by size: 

                1040     821     719     190      83
 
BspHI T'CATG_A

Cuts at:      0      92    1148    2853 
   Size:         92    1056    1705

  Fragments arranged by size: 

                1705    1056      92
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1761    2853 
   Size:       1761    1092
 
BsrBI CCG'CTC

Cuts at:      0    1617    2853 
   Size:       1617    1236
 
BsrDI GCAATG_nn'

Cuts at:      0     431     432    1475    2853 
   Size:        431       1    1043    1378

  Fragments arranged by size: 

                1378    1043     431       1
 
BsrGI T'GTAC_A

Cuts at:      0    2573    2853 
   Size:       2573     280
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1006    1900    2853 
   Size:       1006     894     953

  Fragments arranged by size: 

                1006     953     894
 
BstEII G'GTnAC_C

Cuts at:      0     536     642    2853 
   Size:        536     106    2211

  Fragments arranged by size: 

                2211     536     106
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     549    1818    2853 
   Size:        549    1269    1035

  Fragments arranged by size: 

                1269    1035     549
 
BstYI r'GATC_y

Cuts at:      0    1257    1401    2001    2639    2853 
   Size:       1257     144     600     638     214

  Fragments arranged by size: 

                1257     638     600     214     144
 
Bsu36I CC'TnA_GG

Cuts at:      0    2153    2853 
   Size:       2153     700
 
BtgI C'CryG_G

Cuts at:      0      52    1241    1280    2853 
   Size:         52    1189      39    1573

  Fragments arranged by size: 

                1573    1189      52      39
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1931    2853 
   Size:       1931     922
 
BtsI GCAGTG_nn'

Cuts at:      0    1296    1538    2248    2615    2853 
   Size:       1296     242     710     367     238

  Fragments arranged by size: 

                1296     710     367     242     238
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1044    1079    2853 
   Size:       1044      35    1774

  Fragments arranged by size: 

                1774    1044      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2593    2853 
   Size:       2593     260
 
EaeI y'GGCC_r

Cuts at:      0     156     891    2135    2654    2853 
   Size:        156     735    1244     519     199

  Fragments arranged by size: 

                1244     735     519     199     156
 
EarI CTCTTCn'nnn_

Cuts at:      0     452     620    1319    1439    2153    2853 
   Size:        452     168     699     120     714     700

  Fragments arranged by size: 

                 714     700     699     452     168     120
 
EcoICRI GAG'CTC

Cuts at:      0     819    2853 
   Size:        819    2034
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     488     692     940    1079    1196    1532    1817 
   Size:        149     339     204     248     139     117     336     285

Cuts at:   1817    1898    2214    2726    2853 
   Size:         81     316     512     127

  Fragments arranged by size: 

                 512     339     336     316     285     248     204     149
                 139     127     117      81
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     919    2853 
   Size:        919    1934
 
EcoO109I rG'GnC_Cy

Cuts at:      0     933    2853 
   Size:        933    1920
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      40      72     160     192     559     591    1114    1146 
   Size:         40      32      88      32     367      32     523      32

Cuts at:   1146    1561    1593    2853 
   Size:        415      32    1260

  Fragments arranged by size: 

                1260     523     415     367      88      40      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1222    2483    2853 
   Size:       1222    1261     370

  Fragments arranged by size: 

                1261    1222     370
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    1341    1446    2853 
   Size:        637     704     105    1407

  Fragments arranged by size: 

                1407     704     637     105
 
MscI TGG'CCA

Cuts at:      0     893    2656    2853 
   Size:        893    1763     197

  Fragments arranged by size: 

                1763     893     197
 
MslI CAynn'nnrTG

Cuts at:      0     547    1147    1816    2399    2608    2853 
   Size:        547     600     669     583     209     245

  Fragments arranged by size: 

                 669     600     583     547     245     209
 
MspA1I CmG'CkG

Cuts at:      0    1876    2527    2819    2853 
   Size:       1876     651     292      34

  Fragments arranged by size: 

                1876     651     292      34
 
NcoI C'CATG_G

Cuts at:      0      52    1241    1280    2853 
   Size:         52    1189      39    1573

  Fragments arranged by size: 

                1573    1189      52      39
 
NspI r_CATG'y

Cuts at:      0    1968    2853 
   Size:       1968     885
 
PasI CC'CwG_GG

Cuts at:      0    1592    2853 
   Size:       1592    1261
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1981    2853 
   Size:       1981     872
 
PfoI T'CCnGG_A

Cuts at:      0     917    2853 
   Size:        917    1936
 
PpuMI rG'GwC_Cy

Cuts at:      0     933    2853 
   Size:        933    1920
 
PstI C_TGCA'G

Cuts at:      0    1758    1875    2255    2388    2420    2853 
   Size:       1758     117     380     133      32     433

  Fragments arranged by size: 

                1758     433     380     133     117      32
 
PvuII CAG'CTG

Cuts at:      0    1876    2527    2853 
   Size:       1876     651     326

  Fragments arranged by size: 

                1876     651     326
 
SacI G_AGCT'C

Cuts at:      0     821    2853 
   Size:        821    2032
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1439    2853 
   Size:       1439    1414
 
ScaI AGT'ACT

Cuts at:      0     202    1869    2853 
   Size:        202    1667     984

  Fragments arranged by size: 

                1667     984     202
 
SfcI C'TryA_G

Cuts at:      0     485     563    1118    1754    1871    2251    2313    2384 
   Size:        485      78     555     636     117     380      62      71

Cuts at:   2384    2416    2853 
   Size:         32     437

  Fragments arranged by size: 

                 636     555     485     437     380     117      78      71
                  62      32
 
SmaI CCC'GGG

Cuts at:      0     651    2853 
   Size:        651    2202
 
StuI AGG'CCT

Cuts at:      0    2469    2853 
   Size:       2469     384
 
StyI C'CwwG_G

Cuts at:      0      52    1241    1280    2853 
   Size:         52    1189      39    1573

  Fragments arranged by size: 

                1573    1189      52      39
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     987    2853 
   Size:        987    1866
 
TatI w'GTAC_w

Cuts at:      0     200     904    1867    2573    2853 
   Size:        200     704     963     706     280

  Fragments arranged by size: 

                 963     706     704     280     200
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     231     248     263     280     666     690     698     722 
   Size:        231      17      15      17     386      24       8      24

Cuts at:    722    2853 
   Size:       2131

  Fragments arranged by size: 

                2131     386     231      24      24      17      17      15
                   8
 
Tth111I GACn'n_nGTC

Cuts at:      0    2755    2853 
   Size:       2755      98
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1248    2853 
   Size:       1248    1605
 
XmaI C'CCGG_G

Cuts at:      0     649    2853 
   Size:        649    2204
 
XmnI GAAnn'nnTTC

Cuts at:      0     383    2333    2853 
   Size:        383    1950     520

  Fragments arranged by size: 

                1950     520     383
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApoI     AvaI    BamHI    BanII     BbsI
     BclI    BglII     BlpI     BmrI     BplI     BpmI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BstAPI   BstEII    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PfoI
    PpuMI     PstI    PvuII     SacI     SapI     ScaI     SfcI     SmaI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI Bme1580I    BmgBI     BmtI   Bpu10I    BpuEI    BsaAI
    BsaWI    BsiEI    BsiWI    BsmBI    BspEI    BsrFI   BssHII    BssSI
    BstBI  BstZ17I     ClaI     DraI   DraIII     EagI     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     XbaI     XhoI     ZraI
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