Restriction map for pF1KB3644
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AccII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AocI        ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvaI        AvrII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BclI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HgaI        HincII      
HindII      HpaI        KasI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NarI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII           3  GACGTC               GACGT^C
Acc65I        487  GGTACC               G^GTACC
AccI          718  GTAGAC               GT^[AC][GT]AC
AcyI            3  GACGTC               G[GA]^CG[CT]C
Alw44I        334  GTGCAC               G^TGCAC
AlwNI         725  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       140  AGCGCT               AGC^GCT
ApaI           63  GGGCCC               GGGCC^C
ApaLI         334  GTGCAC               G^TGCAC
Asp718I       487  GGTACC               G^GTACC
BalI          425  TGGCCA               TGG^CCA
BanI          487  GGTACC               G^G[CT][GA]CC
BanII          63  GGGCCC               G[GA]GC[CT]^C
BciVI          71  GGATAC               GTATCC(6/5)
BfaI         1016  CTAG                 C^TAG
BglII         798  AGATCT               A^GATCT
BsaHI           3  GACGTC               G[GA]^CG[CT]C
BspHI         587  TCATGA               T^CATGA
BsrFI          50  GCCGGC               [GA]^CCGG[CT]
BstAPI        575  GCAAGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         461  CCACCATCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         50  GCCGGC               [GA]^CCGG[CT]
DrdI          855  GACAGTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      140  AGCGCT               AGC^GCT
EcoNI         900  CCTGCCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          522  GCGGG                CCCGC(4/6)
Hin1I           3  GACGTC               G[GA]^CG[CT]C
HindIII       199  AAGCTT               A^AGCTT
KpnI          487  GGTACC               GGTAC^C
MaeI         1016  CTAG                 C^TAG
MluNI         425  TGGCCA               TGG^CCA
MscI          425  TGGCCA               TGG^CCA
MseI         1081  TTAA                 T^TAA
MslI          177  CATTGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        448  CAGCTG               C[AC]G^C[GT]G
NaeI           50  GCCGGC               GCC^GGC
NgoMIV         50  GCCGGC               G^CCGGC
PvuII         448  CAGCTG               CAG^CTG
RcaI          587  TCATGA               T^CATGA
SapI          207  GCTCTTC              GCTCTTC(1/4)
SexAI         695  ACCAGGT              A^CC[AT]GGT
SfaNI        1124  GCATC                GCATC(5/9)
SphI          549  GCATGC               GCATG^C
TfiI          370  GAATC                G^A[AT]TC
Tru9I        1081  TTAA                 T^TAA
XcmI          375  CCAAACCACCATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

BbvI          107  GCAGC                GCAGC(8/12)
              629  GCTGC               
BcnI          303  CCCGG                CC^[GC]GG
              509  CCCGG               
BpmI          289  CTGGAG               CTGGAG(16/14)
              347  CTGGAG              
BsrDI         129  CATTGC               GCAATG(2/0)
             1130  GCAATG              
BstF5I        465  CATCC                GGATG(2/0)
              684  CATCC               
BstYI         798  AGATCT               [GA]^GATC[CT]
              911  AGATCC              
DraII         158  AGGACCT              [GA]G^G[ACGT]CC[CT]
              281  GGGACCT             
EaeI          305  CGGCCA               [CT]^GGCC[GA]
              425  TGGCCA              
EcoO109I      158  AGGACCT              [GA]G^G[ACGT]CC[CT]
              281  GGGACCT             
EcoT14I        14  CCAAGG               C^C[AT][AT]GG
              668  CCAAGG              
Fnu4HI        107  GCAGC                GC^[ACGT]GC
              629  GCTGC               
FokI          465  CATCC                GGATG(9/13)
              684  CATCC               
HaeII         140  AGCGCT               [GA]GCGC^[CT]
              191  GGCGCT              
ItaI          107  GCAGC                GC^[ACGT]GC
              629  GCTGC               
MaeII           4  ACGT                 A^CGT
              113  ACGT                
MflI          798  AGATCT               [GA]^GATC[CT]
              911  AGATCC              
NciI          303  CCCGG                CC^[GC]GG
              509  CCCGG               
NspI          182  ACATGC               [GA]CATG^[CT]
              549  GCATGC              
PpuMI         158  AGGACCT              [GA]G^G[AT]CC[CT]
              281  GGGACCT             
SmlI          154  CTCAAG               C^T[CT][GA]AG
             1063  CTCAAG              
StyI           14  CCAAGG               C^C[AT][AT]GG
              668  CCAAGG              
TseI          107  GCAGC                G^C[AT]GC
              629  GCTGC               
XhoII         798  AGATCT               [GA]^GATC[CT]
              911  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AvaII       AviII       BmyI        BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EarI        EcoRII      FspI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       Tsp45I      TspRI       
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