Restriction map for pF1KB3644
 (Linear) (Six-base) MAPSORT of: KIBB3644.seq  Check: 6832  from: 1  to: 1137



KIBB3644.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     945    1137 
   Size:        945     192
 
AatII G_ACGT'C

Cuts at:      0       7    1137 
   Size:          7    1130
 
AccI GT'mk_AC

Cuts at:      0     719    1137 
   Size:        719     418
 
Acc65I G'GTAC_C

Cuts at:      0     487    1137 
   Size:        487     650
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     637    1137 
   Size:        637     500
 
AfeI AGC'GCT

Cuts at:      0     142    1137 
   Size:        142     995
 
AlwNI CAG_nnn'CTG

Cuts at:      0     730    1137 
   Size:        730     407
 
ApaI G_GGCC'C

Cuts at:      0      67    1137 
   Size:         67    1070
 
ApaLI G'TGCA_C

Cuts at:      0     334    1137 
   Size:        334     803
 
BanI G'GyrC_C

Cuts at:      0     487    1137 
   Size:        487     650
 
BanII G_rGCy'C

Cuts at:      0      67    1137 
   Size:         67    1070
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      65    1137 
   Size:         65    1072
 
BglII A'GATC_T

Cuts at:      0     798    1137 
   Size:        798     339
 
Bme1580I G_kGCm'C

Cuts at:      0      67     338    1113    1137 
   Size:         67     271     775      24

  Fragments arranged by size: 

                 775     271      67      24
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     373    1137 
   Size:        373     764
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     368    1137 
   Size:        310      58     769

  Fragments arranged by size: 

                 769     310      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139    1048    1137 
   Size:        139     909      89

  Fragments arranged by size: 

                 909     139      89
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     523     775    1043    1137 
   Size:        523     252     268      94

  Fragments arranged by size: 

                 523     268     252      94
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1137 
   Size:          4    1133
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     610     922    1087    1137 
   Size:        610     312     165      50

  Fragments arranged by size: 

                 610     312     165      50
 
BseYI C'CCAG_C

Cuts at:      0     446    1137 
   Size:        446     691
 
BsiHKAI G_wGCw'C

Cuts at:      0     128     338     458    1137 
   Size:        128     210     120     679

  Fragments arranged by size: 

                 679     210     128     120
 
Bsp1286I G_dGCh'C

Cuts at:      0      67     128     338     458    1113    1137 
   Size:         67      61     210     120     655      24

  Fragments arranged by size: 

                 655     210     120      67      61      24
 
BspHI T'CATG_A

Cuts at:      0     587    1137 
   Size:        587     550
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     293     908     945    1137 
   Size:        293     615      37     192

  Fragments arranged by size: 

                 615     293     192      37
 
BsrDI GCAATG_nn'

Cuts at:      0     128    1137 
   Size:        128    1009
 
BsrFI r'CCGG_y

Cuts at:      0      50    1137 
   Size:         50    1087
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     581    1137 
   Size:        581     556
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     468    1137 
   Size:        468     669
 
BstYI r'GATC_y

Cuts at:      0     798     911    1137 
   Size:        798     113     226

  Fragments arranged by size: 

                 798     226     113
 
BtsI GCAGTG_nn'

Cuts at:      0     193     819    1113    1137 
   Size:        193     626     294      24

  Fragments arranged by size: 

                 626     294     193      24
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     861    1137 
   Size:        861     276
 
EaeI y'GGCC_r

Cuts at:      0     305     425    1137 
   Size:        305     120     712

  Fragments arranged by size: 

                 712     305     120
 
EarI CTCTTCn'nnn_

Cuts at:      0     214     364     812    1137 
   Size:        214     150     448     325

  Fragments arranged by size: 

                 448     325     214     150
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      96    1137 
   Size:         96    1041
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     368     637    1137 
   Size:        310      58     269     500

  Fragments arranged by size: 

                 500     310     269      58
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     904    1137 
   Size:        904     233
 
EcoO109I rG'GnC_Cy

Cuts at:      0     159     282    1137 
   Size:        159     123     855

  Fragments arranged by size: 

                 855     159     123
 
FspI TGC'GCA

Cuts at:      0     610     889    1123    1137 
   Size:        610     279     234      14

  Fragments arranged by size: 

                 610     279     234      14
 
FspAI rTGC'GCAy

Cuts at:      0     889    1123    1137 
   Size:        889     234      14

  Fragments arranged by size: 

                 889     234      14
 
HaeII r_GCGC'y

Cuts at:      0     144     195    1137 
   Size:        144      51     942

  Fragments arranged by size: 

                 942     144      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     712     744    1137 
   Size:        712      32     393

  Fragments arranged by size: 

                 712     393      32
 
HindIII A'AGCT_T

Cuts at:      0     199    1137 
   Size:        199     938
 
KpnI G_GTAC'C

Cuts at:      0     491    1137 
   Size:        491     646
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1049    1137 
   Size:       1049      88
 
MscI TGG'CCA

Cuts at:      0     427    1137 
   Size:        427     710
 
MslI CAynn'nnrTG

Cuts at:      0     181    1137 
   Size:        181     956
 
MspA1I CmG'CkG

Cuts at:      0     450    1137 
   Size:        450     687
 
NaeI GCC'GGC

Cuts at:      0      52    1137 
   Size:         52    1085
 
NgoMIV G'CCGG_C

Cuts at:      0      50    1137 
   Size:         50    1087
 
NspI r_CATG'y

Cuts at:      0     186     553    1137 
   Size:        186     367     584

  Fragments arranged by size: 

                 584     367     186
 
PasI CC'CwG_GG

Cuts at:      0      67    1137 
   Size:         67    1070
 
PfoI T'CCnGG_A

Cuts at:      0     467    1137 
   Size:        467     670
 
PpuMI rG'GwC_Cy

Cuts at:      0     159     282    1137 
   Size:        159     123     855

  Fragments arranged by size: 

                 855     159     123
 
PspOMI G'GGCC_C

Cuts at:      0      63    1137 
   Size:         63    1074
 
PvuII CAG'CTG

Cuts at:      0     450    1137 
   Size:        450     687
 
SapI GCTCTTCn'nnn_

Cuts at:      0     214    1137 
   Size:        214     923
 
SexAI A'CCwGG_T

Cuts at:      0     695    1137 
   Size:        695     442
 
SmlI C'TyrA_G

Cuts at:      0     154    1063    1137 
   Size:        154     909      74

  Fragments arranged by size: 

                 909     154      74
 
SphI G_CATG'C

Cuts at:      0     553    1137 
   Size:        553     584
 
StyI C'CwwG_G

Cuts at:      0      14     668    1137 
   Size:         14     654     469

  Fragments arranged by size: 

                 654     469      14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     382    1137 
   Size:        382     755
 
ZraI GAC'GTC

Cuts at:      0       5    1137 
   Size:          5    1132
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AlwNI     ApaI
    ApaLI     BanI    BanII    BciVI    BglII Bme1580I     BplI     BpmI
    BpuEI     BsaI    BsaHI    BseRI    BseYI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtsI     DrdI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    FspAI
    HaeII    Hin4I  HindIII     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI   NgoMIV     NspI     PasI     PfoI    PpuMI   PspOMI    PvuII
     SapI    SexAI     SmlI     SphI     StyI     XcmI     ZraI

 Enzymes that do not cut: 

     AclI    AflII   AflIII     AgeI     AhdI     AleI     AlfI     AloI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmrI     BmtI   Bpu10I    BsaAI    BsaBI    BsaWI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI   HincII     HpaI     KasI     MfeI     MluI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI
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