Restriction map for pF1KB3626
##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

Acc65I      AccIII      AflIII      AgeI        Alw44I      
AocI        ApaI        ApaLI       AscI        Asp718I     
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BlnI        BlpI        Bpu1102I    BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BsrBI       BssHII      Bst1107I    BstZ17I     Bsu36I      
CelII       ClaI        DrdI        EagI        EclXI       
Eco52I      Eco81I      EcoRV       FbaI        FseI        
HincII      HindII      HpaI        KasI        KpnI        
KspI        MluI        MroI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SwaI        XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AatII        3187  GACGTC               GACGT^C
AccI         1699  GTCTAC               GT^[AC][GT]AC
AccII        1975  CGCG                 CG^CG
AclI          925  AACGTT               AA^CGTT
Aor51HI      2615  AGCGCT               AGC^GCT
AseI         2533  ATTAAT               AT^TAAT
AsnI         2533  ATTAAT               AT^TAAT
AspI         2125  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1643  CCCGAG               C^[CT]CG[GA]G
AviII        1592  TGCGCA               TGC^GCA
BanI         1468  GGTGCC               G^G[CT][GA]CC
BbvCI        1392  GCTGAGG              CCTCAGC(-5/-2)
BglI          138  GCCCCTCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2780  AGATCT               A^GATCT
BsaAI        1387  TACGTG               [CT]AC^GT[GA]
BsaBI        1252  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI        1725  CGATCG               CG[GA][CT]^CG
BsmI         2251  GAATGC               GAATGC(1/-1)
BsoBI        1643  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1333  TGTACA               T^GTACA
BsrDI          29  GCAATG               GCAATG(2/0)
BsrFI        2423  GCCGGT               [GA]^CCGG[CT]
BsrGI        1333  TGTACA               T^GTACA
BstBI         909  TTCGAA               TT^CGAA
BstEII       2746  GGTAACC              G^GT[ACGT]ACC
BstPI        2746  GGTAACC              G^GT[ACGT]ACC
BstUI        1975  CGCG                 CG^CG
Cfr10I       2423  GCCGGT               [GA]^CCGG[CT]
CpoI         1535  CGGACCG              CG^G[AT]CCG
DraI         3456  TTTAAA               TTT^AAA
Eco47III     2615  AGCGCT               AGC^GCT
EcoO65I      2746  GGTAACC              G^GT[ACGT]ACC
EcoRI        3397  GAATTC               G^AATTC
EcoT22I      2253  ATGCAT               ATGCA^T
FauI         2474  CCCGC                CCCGC(4/6)
FspI         1592  TGCGCA               TGC^GCA
HaeII        2615  AGCGCT               [GA]GCGC^[CT]
MamI         1252  GATGCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1975  CGCG                 CG^CG
NsiI         2253  ATGCAT               ATGCA^T
NspI          199  GCATGC               [GA]CATG^[CT]
NspV          909  TTCGAA               TT^CGAA
PflFI        2125  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        2533  ATTAAT               AT^TAAT
Psp1406I      925  AACGTT               AA^CGTT
PstI         2673  CTGCAG               CTGCA^G
PvuI         1725  CGATCG               CGAT^CG
RsrII        1535  CGGACCG              CG^G[AT]CCG
SapI         2545  GAAGAGC              GCTCTTC(1/4)
SexAI        2126  ACCTGGT              A^CC[AT]GGT
SfuI          909  TTCGAA               TT^CGAA
SphI          199  GCATGC               GCATG^C
SspBI        1333  TGTACA               T^GTACA
SspI          852  AATATT               AAT^ATT
Tth111I      2125  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflII        2784  CTTAAG               C^TTAAG
             2890  CTTAAG              
AhdI          295  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3187  GACGTCTGGTC         
AlwNI        1585  CAGATCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2582  CAGCAACTG           
Asp700I      1476  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2896  GAATATCTTC          
AspEI         295  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3187  GACGTCTGGTC         
BamHI        1730  GGATCC               G^GATCC
             2630  GGATCC              
BbsI           69  GAAGAC               GAAGAC(2/6)
             2836  GAAGAC              
BfrI         2784  CTTAAG               C^TTAAG
             2890  CTTAAG              
BpuAI          69  GAAGAC               GAAGAC(2/6)
             2836  GAAGAC              
BsaWI         320  ACCGGA               [AT]^CCGG[AT]
             2039  ACCGGA              
BssSI        1195  CACGAG               CACGAG(-5/-1)
             1776  CACGAG              
BstAPI       1584  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3213  GCATGAGCTGC         
CfoI         1593  GCGC                 GCG^C
             2616  GCGC                
DraIII       1945  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3043  CACCAAGTG           
Eam1105I      295  GACAAGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3187  GACGTCTGGTC         
EcoNI          94  CCTGAGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1734  CCTCAAGAAGG         
HhaI         1593  GCGC                 GCG^C
             2616  GCGC                
HinP1I       1593  GCGC                 G^CGC
             2616  GCGC                
HindIII      2539  AAGCTT               A^AGCTT
             3420  AAGCTT              
MfeI          342  CAATTG               C^AATTG
             3080  CAATTG              
MunI          342  CAATTG               C^AATTG
             3080  CAATTG              
PpuMI        1798  GGGACCC              [GA]G^G[AT]CC[CT]
             2016  GGGTCCT             
PvuII        1714  CAGCTG               CAG^CTG
             2089  CAGCTG              
SacI           60  GAGCTC               GAGCT^C
             2668  GAGCTC              
SfcI         1518  CTACAG               C^T[GA][CT]AG
             2673  CTGCAG              
StuI         1202  AGGCCT               AGG^CCT
             2503  AGGCCT              
XcmI         1655  CCAACCTGCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3045  CCAAGTGAAAATTGG     
XmnI         1476  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2896  GAATATCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaII       BalI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspHI       BspMI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        Hin1I       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII       
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