Restriction map for pF1KB3523
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp718I     AviII       AvrII       BamHI       BbrPI       
BciVI       BclI        BfrI        BlnI        BsaAI       
BsaBI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NaeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfuI        SgrAI       SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII         290  CGCG                 CG^CG
AcsI         1279  GAATTC               [GA]^AATT[CT]
AhdI         2372  GACCTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2229  AGCGCT               AGC^GCT
ApoI         1279  GAATTC               [GA]^AATT[CT]
AspEI        2372  GACCTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI          230  GGCGCC               GGCGC^C
BbvCI        2053  GCTGAGG              CCTCAGC(-5/-2)
BcgI         2292  CGACCTGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         2129  CTAG                 C^TAG
BglII        1079  AGATCT               A^GATCT
BsiEI         467  CGGCCG               CG[GA][CT]^CG
BsmI         1089  GAATGC               GAATGC(1/-1)
BstAPI        489  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        438  GGTGACC              G^GT[ACGT]ACC
BstPI         438  GGTGACC              G^GT[ACGT]ACC
BstUI         290  CGCG                 CG^CG
DraIII        694  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1699  GACTACCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          467  CGGCCG               C^GGCCG
Eam1105I     2372  GACCTAAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         467  CGGCCG               C^GGCCG
Eco47III     2229  AGCGCT               AGC^GCT
Eco52I        467  CGGCCG               C^GGCCG
EcoO65I       438  GGTGACC              G^GT[ACGT]ACC
EcoRI        1279  GAATTC               G^AATTC
HincII       1447  GTTGAC               GT[CT]^[GA]AC
HindII       1447  GTTGAC               GT[CT]^[GA]AC
KasI          230  GGCGCC               G^GCGCC
MaeI         2129  CTAG                 C^TAG
MseI         1490  TTAA                 T^TAA
MvnI          290  CGCG                 CG^CG
NarI          230  GGCGCC               GG^CGCC
NdeI          779  CATATG               CA^TATG
SapI         1029  GCTCTTC              GCTCTTC(1/4)
SbfI         1647  CCTGCAGG             CCTGCA^GG
SexAI        1703  ACCTGGT              A^CC[AT]GGT
SfoI          230  GGCGCC               GGC^GCC
SphI          534  GCATGC               GCATG^C
Sse8387I     1647  CCTGCAGG             CCTGCA^GG
Tru9I        1490  TTAA                 T^TAA
XcmI         2189  CCATTGTCTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   44 enzymes) #
###################################

AccI          205  GTCTAC               GT^[AC][GT]AC
             2029  GTCTAC              
AccIII        983  TCCGGA               T^CCGGA
             1869  TCCGGA              
AcyI          230  GGCGCC               G[GA]^CG[CT]C
             2011  GACGCC              
AflIII        143  ACACGT               A^C[GA][CT]GT
              770  ACGTGT              
AlwNI         257  CAGGCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1661  CAGTAGCTG           
AocI           55  CCTAAGG              CC^T[ACGT]AGG
             2170  CCTGAGG             
ApaI         1571  GGGCCC               GGGCC^C
             1610  GGGCCC              
Asp700I      1077  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1561  GAACTGGTTC          
AvaI          395  CCCGGG               C^[CT]CG[GA]G
             2210  CCCGGG              
BlpI          817  GCTGAGC              GC^T[ACGT]AGC
             2333  GCTCAGC             
Bpu1102I      817  GCTGAGC              GC^T[ACGT]AGC
             2333  GCTCAGC             
BsaHI         230  GGCGCC               G[GA]^CG[CT]C
             2011  GACGCC              
BsaI           65  GAGACC               GGTCTC(1/5)
              266  GAGACC              
BseAI         983  TCCGGA               T^CCGGA
             1869  TCCGGA              
BsgI          446  GTGCAG               GTGCAG(16/14)
              496  GTGCAG              
BsoBI         395  CCCGGG               C^[CT]CG[GA]G
             2210  CCCGGG              
BspEI         983  TCCGGA               T^CCGGA
             1869  TCCGGA              
BspMI         522  GCAGGT               ACCTGC(4/8)
             1620  GCAGGT              
BsrBI        2331  CCGCTC               CCGCTC(-3/-3)
             1198  GAGCGG              
BsrDI        1233  CATTGC               GCAATG(2/0)
             1848  CATTGC              
BsrFI         193  ACCGGC               [GA]^CCGG[CT]
             1292  ACCGGC              
BstYI        1079  AGATCT               [GA]^GATC[CT]
             2273  GGATCT              
Bsu36I         55  CCTAAGG              CC^T[ACGT]AGG
             2170  CCTGAGG             
CelII         817  GCTGAGC              GC^T[ACGT]AGC
             2333  GCTCAGC             
Cfr10I        193  ACCGGC               [GA]^CCGG[CT]
             1292  ACCGGC              
EarI         1030  CTCTTC               CTCTTC(1/4)
             1537  GAAGAG              
Eco81I         55  CCTAAGG              CC^T[ACGT]AGG
             2170  CCTGAGG             
EcoNI         250  CCTGCTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              324  CCTTAGCGAGG         
HgaI          662  GACGC                GACGC(5/10)
             2011  GACGC               
Hin1I         230  GGCGCC               G[GA]^CG[CT]C
             2011  GACGCC              
Ksp632I      1030  CTCTTC               CTCTTC(1/4)
             1537  GAAGAG              
MflI         1079  AGATCT               [GA]^GATC[CT]
             2273  GGATCT              
MroI          983  TCCGGA               T^CCGGA
             1869  TCCGGA              
NspI          534  GCATGC               [GA]CATG^[CT]
              845  ACATGC              
PstI         1648  CTGCAG               CTGCA^G
             1819  CTGCAG              
SacI         1356  GAGCTC               GAGCT^C
             1786  GAGCTC              
SfiI         1602  GGCCTACGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1644  GGCCCTGCAGGCC       
SmaI          395  CCCGGG               CCC^GGG
             2210  CCCGGG              
SmlI          547  CTCAAG               C^T[CT][GA]AG
              949  CTCAAG              
StuI         1601  AGGCCT               AGG^CCT
             2219  AGGCCT              
XhoII        1079  AGATCT               [GA]^GATC[CT]
             2273  GGATCT              
XmaCI         395  CCCGGG               C^CCGGG
             2210  CCCGGG              
XmaI          395  CCCGGG               C^CCGGG
             2210  CCCGGG              
XmnI         1077  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1561  GAACTGGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
AspI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflFI       PflMI       PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
TseI        Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      
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