Restriction map for pF1KB3523
 (Linear) (Six-base) MAPSORT of: KIBB3523.seq  Check: 4188  from: 1  to: 2574



KIBB3523.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     513    2574 
   Size:        513    2061
 
AccI GT'mk_AC

Cuts at:      0     206    2030    2574 
   Size:        206    1824     544

  Fragments arranged by size: 

                1824     544     206
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     525     871    1017    1569    2262    2324    2574 
   Size:        525     346     146     552     693      62     250

  Fragments arranged by size: 

                 693     552     525     346     250     146      62
 
AfeI AGC'GCT

Cuts at:      0    2231    2574 
   Size:       2231     343
 
AflIII A'CryG_T

Cuts at:      0     143     770    2574 
   Size:        143     627    1804

  Fragments arranged by size: 

                1804     627     143
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2377    2574 
   Size:       2377     197
 
AleI CACnn'nnGTG

Cuts at:      0    1126    2574 
   Size:       1126    1448
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     862    1319    1663    1834    2574 
   Size:        862     457     344     171     740

  Fragments arranged by size: 

                 862     740     457     344     171
 
AlwNI CAG_nnn'CTG

Cuts at:      0     262    1666    2574 
   Size:        262    1404     908

  Fragments arranged by size: 

                1404     908     262
 
ApaI G_GGCC'C

Cuts at:      0    1575    1614    2574 
   Size:       1575      39     960

  Fragments arranged by size: 

                1575     960      39
 
ApoI r'AATT_y

Cuts at:      0    1279    2574 
   Size:       1279    1295
 
AvaI C'yCGr_G

Cuts at:      0     395    2210    2574 
   Size:        395    1815     364

  Fragments arranged by size: 

                1815     395     364
 
BanI G'GyrC_C

Cuts at:      0     230     745    1203    1578    1746    2431    2574 
   Size:        230     515     458     375     168     685     143

  Fragments arranged by size: 

                 685     515     458     375     230     168     143
 
BanII G_rGCy'C

Cuts at:      0    1360    1575    1614    1790    1880    2143    2574 
   Size:       1360     215      39     176      90     263     431

  Fragments arranged by size: 

                1360     431     263     215     176      90      39
 
BbeI G_GCGC'C

Cuts at:      0     234    2574 
   Size:        234    2340
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     868    1916    1922    2574 
   Size:        868    1048       6     652

  Fragments arranged by size: 

                1048     868     652       6
 
BbvCI CC'TCA_GC

Cuts at:      0    2054    2574 
   Size:       2054     520
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2281    2315    2574 
   Size:       2281      34     259

  Fragments arranged by size: 

                2281     259      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     291    1609    1651    2574 
   Size:        291    1318      42     923

  Fragments arranged by size: 

                1318     923     291      42
 
BglII A'GATC_T

Cuts at:      0    1079    2574 
   Size:       1079    1495
 
BlpI GC'TnA_GC

Cuts at:      0     818    2334    2574 
   Size:        818    1516     240

  Fragments arranged by size: 

                1516     818     240
 
Bme1580I G_kGCm'C

Cuts at:      0     748    1475    1575    1583    1614    1751    1813    2434 
   Size:        748     727     100       8      31     137      62     621

Cuts at:   2434    2574 
   Size:        140

  Fragments arranged by size: 

                 748     727     621     140     137     100      62      31
                   8
 
BmgBI CAC'GTC

Cuts at:      0     771    2574 
   Size:        771    1803
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     415    1752    1794    2079    2574 
   Size:        415    1337      42     285     495

  Fragments arranged by size: 

                1337     495     415     285      42
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2349    2574 
   Size:       2349     225
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     284     406    1093    1528    1618    1656    2574 
   Size:         83     201     122     687     435      90      38     918

  Fragments arranged by size: 

                 918     687     435     201     122      90      83      38
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     325    1616    2054    2574 
   Size:        325    1291     438     520

  Fragments arranged by size: 

                1291     520     438     325
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     934    2574 
   Size:        532     402    1640

  Fragments arranged by size: 

                1640     532     402
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      59     260    2574 
   Size:         59     201    2314

  Fragments arranged by size: 

                2314     201      59
 
BsaHI Gr'CG_yC

Cuts at:      0     231    2012    2574 
   Size:        231    1781     562

  Fragments arranged by size: 

                1781     562     231
 
BsaWI w'CCGG_w

Cuts at:      0     983    1179    1869    2574 
   Size:        983     196     690     705

  Fragments arranged by size: 

                 983     705     690     196
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      56      86     463     493     755     785    1903    1933 
   Size:         56      30     377      30     262      30    1118      30

Cuts at:   1933    2574 
   Size:        641

  Fragments arranged by size: 

                1118     641     377     262      56      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      31     256     346    1513    1531    1555    1798    1846 
   Size:         31     225      90    1167      18      24     243      48

Cuts at:   1846    1849    1932    2574 
   Size:          3      83     642

  Fragments arranged by size: 

                1167     642     243     225      90      83      48      31
                  24      18       3
 
BseYI C'CCAG_C

Cuts at:      0     741     808    1137    1439    2574 
   Size:        741      67     329     302    1135

  Fragments arranged by size: 

                1135     741     329     302      67
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     467     517    2574 
   Size:        467      50    2057

  Fragments arranged by size: 

                2057     467      50
 
BsiEI CG_ry'CG

Cuts at:      0     470    2574 
   Size:        470    2104
 
BsiHKAI G_wGCw'C

Cuts at:      0     598     707     824    1031    1360    1642    1790    2574 
   Size:        598     109     117     207     329     282     148     784

  Fragments arranged by size: 

                 784     598     329     282     207     148     117     109
            
 
BsmI GAATG_Cn'

Cuts at:      0    1095    2574 
   Size:       1095    1479
 
Bsp1286I G_dGCh'C

Cuts at:      0     598     707     748     824    1031    1360    1475    1575 
   Size:        598     109      41      76     207     329     115     100

Cuts at:   1575    1583    1614    1642    1751    1790    1813    1880    2143 
   Size:          8      31      28     109      39      23      67     263

Cuts at:   2143    2434    2574 
   Size:        291     140

  Fragments arranged by size: 

                 598     329     291     263     207     140     115     109
                 109     100      76      67      41      39      31      28
                  23       8
 
BspEI T'CCGG_A

Cuts at:      0     983    1869    2574 
   Size:        983     886     705

  Fragments arranged by size: 

                 983     886     705
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     513    1611    2574 
   Size:        513    1098     963

  Fragments arranged by size: 

                1098     963     513
 
BsrBI CCG'CTC

Cuts at:      0    1200    2333    2574 
   Size:       1200    1133     241

  Fragments arranged by size: 

                1200    1133     241
 
BsrDI GCAATG_nn'

Cuts at:      0    1232    1847    2574 
   Size:       1232     615     727

  Fragments arranged by size: 

                1232     727     615
 
BsrFI r'CCGG_y

Cuts at:      0     193    1292    2574 
   Size:        193    1099    1282

  Fragments arranged by size: 

                1282    1099     193
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     495    2574 
   Size:        495    2079
 
BstEII G'GTnAC_C

Cuts at:      0     438    2574 
   Size:        438    2136
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     294     450     910    1758    2538    2574 
   Size:        294     156     460     848     780      36

  Fragments arranged by size: 

                 848     780     460     294     156      36
 
BstYI r'GATC_y

Cuts at:      0    1079    2273    2574 
   Size:       1079    1194     301

  Fragments arranged by size: 

                1194    1079     301
 
Bsu36I CC'TnA_GG

Cuts at:      0      56    2171    2574 
   Size:         56    2115     403

  Fragments arranged by size: 

                2115     403      56
 
BtgI C'CryG_G

Cuts at:      0    1143    1475    2198    2312    2574 
   Size:       1143     332     723     114     262

  Fragments arranged by size: 

                1143     723     332     262     114
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1205    1937    2574 
   Size:       1205     732     637

  Fragments arranged by size: 

                1205     732     637
 
BtsI GCAGTG_nn'

Cuts at:      0     455     684    1552    2574 
   Size:        455     229     868    1022

  Fragments arranged by size: 

                1022     868     455     229
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1665    1700    2574 
   Size:       1665      35     874

  Fragments arranged by size: 

                1665     874      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     699    2574 
   Size:        699    1875
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1705    2574 
   Size:       1705     869
 
EaeI y'GGCC_r

Cuts at:      0     195     467    1130    1415    2567    2574 
   Size:        195     272     663     285    1152       7

  Fragments arranged by size: 

                1152     663     285     272     195       7
 
EagI C'GGCC_G

Cuts at:      0     467    2574 
   Size:        467    2107
 
EarI CTCTTCn'nnn_

Cuts at:      0    1036    1532    2574 
   Size:       1036     496    1042

  Fragments arranged by size: 

                1042    1036     496
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     237     329    2574 
   Size:         19     218      92    2245

  Fragments arranged by size: 

                2245     218      92      19
 
EcoICRI GAG'CTC

Cuts at:      0    1358    1788    2574 
   Size:       1358     430     786

  Fragments arranged by size: 

                1358     786     430
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      83     284     406     525     871    1017    1093    1528 
   Size:         83     201     122     119     346     146      76     435

Cuts at:   1528    1569    1618    1656    2262    2324    2574 
   Size:         41      49      38     606      62     250

  Fragments arranged by size: 

                 606     435     346     250     201     146     122     119
                  83      76      62      49      41      38
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     254     328    2574 
   Size:        254      74    2246

  Fragments arranged by size: 

                2246     254      74
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51     123     246     456    1254    1610    1611    2371 
   Size:         51      72     123     210     798     356       1     760

Cuts at:   2371    2535    2574 
   Size:        164      39

  Fragments arranged by size: 

                 798     760     356     210     164     123      72      51
                  39       1
 
EcoRI G'AATT_C

Cuts at:      0    1279    2574 
   Size:       1279    1295
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     555    1519    1746    1906    2574 
   Size:        555     964     227     160     668

  Fragments arranged by size: 

                 964     668     555     227     160
 
HaeII r_GCGC'y

Cuts at:      0      25     234    2233    2574 
   Size:         25     209    1999     341

  Fragments arranged by size: 

                1999     341     209      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      56      88     463     495     760     792     811     843 
   Size:         56      32     375      32     265      32      19      32

Cuts at:    843    1903    1935    2265    2297    2335    2363    2367    2395 
   Size:       1060      32     330      32      38      28       4      28

Cuts at:   2395    2474    2506    2574 
   Size:         79      32      68

  Fragments arranged by size: 

                1060     375     330     265      79      68      56      38
                  32      32      32      32      32      32      32      28
                  28      19       4
 
HincII GTy'rAC

Cuts at:      0    1449    2574 
   Size:       1449    1125
 
KasI G'GCGC_C

Cuts at:      0     230    2574 
   Size:        230    2344
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     866    1972    2574 
   Size:        536     330    1106     602

  Fragments arranged by size: 

                1106     602     536     330
 
MscI TGG'CCA

Cuts at:      0    1132    1417    2569    2574 
   Size:       1132     285    1152       5

  Fragments arranged by size: 

                1152    1132     285       5
 
MslI CAynn'nnrTG

Cuts at:      0     136     844    1126    1774    2087    2574 
   Size:        136     708     282     648     313     487

  Fragments arranged by size: 

                 708     648     487     313     282     136
 
MspA1I CmG'CkG

Cuts at:      0     906    1001    1464    1986    2048    2338    2574 
   Size:        906      95     463     522      62     290     236

  Fragments arranged by size: 

                 906     522     463     290     236      95      62
 
NarI GG'CG_CC

Cuts at:      0     231    2574 
   Size:        231    2343
 
NcoI C'CATG_G

Cuts at:      0    1143    1475    2198    2312    2574 
   Size:       1143     332     723     114     262

  Fragments arranged by size: 

                1143     723     332     262     114
 
NdeI CA'TA_TG

Cuts at:      0     780    2574 
   Size:        780    1794
 
NspI r_CATG'y

Cuts at:      0     538     849    2574 
   Size:        538     311    1725

  Fragments arranged by size: 

                1725     538     311
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     699    1989    2069    2565    2574 
   Size:        699    1290      80     496       9

  Fragments arranged by size: 

                1290     699     496      80       9
 
PfoI T'CCnGG_A

Cuts at:      0     800     929    2381    2391    2574 
   Size:        800     129    1452      10     183

  Fragments arranged by size: 

                1452     800     183     129      10
 
PpuMI rG'GwC_Cy

Cuts at:      0      51     246    2371    2535    2574 
   Size:         51     195    2125     164      39

  Fragments arranged by size: 

                2125     195     164      51      39
 
PspOMI G'GGCC_C

Cuts at:      0    1571    1610    2574 
   Size:       1571      39     964

  Fragments arranged by size: 

                1571     964      39
 
PstI C_TGCA'G

Cuts at:      0    1652    1823    2574 
   Size:       1652     171     751

  Fragments arranged by size: 

                1652     751     171
 
PvuII CAG'CTG

Cuts at:      0    1001    1464    1986    2338    2574 
   Size:       1001     463     522     352     236

  Fragments arranged by size: 

                1001     522     463     352     236
 
SacI G_AGCT'C

Cuts at:      0    1360    1790    2574 
   Size:       1360     430     784

  Fragments arranged by size: 

                1360     784     430
 
SanDI GG'GwC_CC

Cuts at:      0      51    2574 
   Size:         51    2523
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1036    2574 
   Size:       1036    1538
 
SbfI CC_TGCA'GG

Cuts at:      0    1652    2574 
   Size:       1652     922
 
SexAI A'CCwGG_T

Cuts at:      0    1703    2574 
   Size:       1703     871
 
SfcI C'TryA_G

Cuts at:      0     254    1648    1819    2574 
   Size:        254    1394     171     755

  Fragments arranged by size: 

                1394     755     254     171
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1609    1651    2574 
   Size:       1609      42     923

  Fragments arranged by size: 

                1609     923      42
 
SfoI GGC'GCC

Cuts at:      0     232    2574 
   Size:        232    2342
 
SmaI CCC'GGG

Cuts at:      0     397    2212    2574 
   Size:        397    1815     362

  Fragments arranged by size: 

                1815     397     362
 
SmlI C'TyrA_G

Cuts at:      0     547     949    2574 
   Size:        547     402    1625

  Fragments arranged by size: 

                1625     547     402
 
SphI G_CATG'C

Cuts at:      0     538    2574 
   Size:        538    2036
 
StuI AGG'CCT

Cuts at:      0    1603    2221    2574 
   Size:       1603     618     353

  Fragments arranged by size: 

                1603     618     353
 
StyI C'CwwG_G

Cuts at:      0    1143    1475    1890    2198    2312    2531    2574 
   Size:       1143     332     415     308     114     219      43

  Fragments arranged by size: 

                1143     415     332     308     219     114      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1138    1453    1734    2475    2574 
   Size:       1138     315     281     741      99

  Fragments arranged by size: 

                1138     741     315     281      99
 
TatI w'GTAC_w

Cuts at:      0     773    1433    2574 
   Size:        773     660    1141

  Fragments arranged by size: 

                1141     773     660
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     755     787    2574 
   Size:        755      32    1787

  Fragments arranged by size: 

                1787     755      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      70     424     796    2574 
   Size:         70     354     372    1778

  Fragments arranged by size: 

                1778     372     354      70
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2196    2574 
   Size:       2196     378
 
XmaI C'CCGG_G

Cuts at:      0     395    2210    2574 
   Size:        395    1815     364

  Fragments arranged by size: 

                1815     395     364
 
XmnI GAAnn'nnTTC

Cuts at:      0    1081    1565    2574 
   Size:       1081     484    1009

  Fragments arranged by size: 

                1081    1009     484
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApaI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspMI    BsrBI    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI    HaeII    Hin4I   HincII     KasI     MmeI     MscI     MslI
   MspA1I     NarI     NcoI     NdeI     NspI    PflMI     PfoI    PpuMI
   PspOMI     PstI    PvuII     SacI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SmaI     SmlI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AloI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BciVI     BclI    BfrBI
     BmtI    BsaAI    BsaBI    BsiWI    BsmBI    BspHI    BsrGI   BssHII
    BssSI    BstBI  BstZ17I     ClaI     DraI    EcoRV     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MfeI     MluI     NaeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI     ScaI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI     XbaI     XhoI     ZraI
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