Restriction map for pF1KB3513
##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AviII       BcgI        BlpI        
Bpu1102I    BseAI       BsiHKAI     BsiWI       BsmI        
Bsp1407I    BspEI       BsrFI       BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        DraIII      
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MluI        
MroI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccI          775  GTCTAC               GT^[AC][GT]AC
AccII         218  CGCG                 CG^CG
AflII        1327  CTTAAG               C^TTAAG
AflIII       1169  ACATGT               A^C[GA][CT]GT
AvaI         2353  CCCGAG               C^[CT]CG[GA]G
AvrII        1656  CCTAGG               C^CTAGG
BalI         1010  TGGCCA               TGG^CCA
BamHI        1538  GGATCC               G^GATCC
BbeI         1525  GGCGCC               GGCGC^C
BbrPI         761  CACGTG               CAC^GTG
BciVI        2940  GTATCC               GTATCC(6/5)
BcnI          180  CCGGG                CC^[GC]GG
BfrI         1327  CTTAAG               C^TTAAG
BglI         3035  GCCCTGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2855  AGATCT               A^GATCT
BlnI         1656  CCTAGG               C^CTAGG
BsaAI         761  CACGTG               [CT]AC^GT[GA]
BsaBI         185  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1151  ACCGGA               [AT]^CCGG[AT]
BsiEI        1790  CGGCCG               CG[GA][CT]^CG
BsmBI         712  GAGACG               CGTCTC(1/5)
BsoBI        2353  CCCGAG               C^[CT]CG[GA]G
BspDI        1738  ATCGAT               AT^CGAT
BspHI        2492  TCATGA               T^CATGA
BspLU11I     1169  ACATGT               A^CATGT
BsrBI        2539  GAGCGG               CCGCTC(-3/-3)
BssSI         197  CTCGTG               CACGAG(-5/-1)
BstAPI       1342  GCAGGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         515  TTCGAA               TT^CGAA
BstUI         218  CGCG                 CG^CG
ClaI         1738  ATCGAT               AT^CGAT
DrdI         1095  GACCTTGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1790  CGGCCG               C^GGCCG
EclXI        1790  CGGCCG               C^GGCCG
Eco52I       1790  CGGCCG               C^GGCCG
EcoNI        1587  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII        1525  GGCGCC               [GA]GCGC^[CT]
KasI         1525  GGCGCC               G^GCGCC
MamI          185  GATTAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1010  TGGCCA               TGG^CCA
MscI         1010  TGGCCA               TGG^CCA
MvnI          218  CGCG                 CG^CG
NarI         1525  GGCGCC               GG^CGCC
NciI          180  CCGGG                CC^[GC]GG
NspI         1169  ACATGT               [GA]CATG^[CT]
NspV          515  TTCGAA               TT^CGAA
PflMI        2580  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         761  CACGTG               CAC^GTG
PmlI          761  CACGTG               CAC^GTG
PpuMI        2074  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI         2492  TCATGA               T^CATGA
SexAI        2956  ACCTGGT              A^CC[AT]GGT
SfoI         1525  GGCGCC               GGC^GCC
SfuI          515  TTCGAA               TT^CGAA
StuI         1982  AGGCCT               AGG^CCT
Van91I       2580  CCAGGCCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI        1582  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2581  CAGGCCCTG           
Asp700I      1687  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2625  GAACGGCTTC          
BanI          754  GGCACC               G^G[CT][GA]CC
             1525  GGCGCC              
BanII        1498  GAGCCC               G[GA]GC[CT]^C
             1759  GGGCTC              
BbvCI         496  CCTCAGC              CCTCAGC(-5/-2)
             2083  CCTCAGC             
BclI          126  TGATCA               T^GATCA
             2546  TGATCA              
BsgI          200  GTGCAG               GTGCAG(16/14)
             2785  GTGCAG              
BspMI        2749  ACCTGC               ACCTGC(4/8)
             1273  GCAGGT              
CfoI          217  GCGC                 GCG^C
             1526  GCGC                
EaeI         1010  TGGCCA               [CT]^GGCC[GA]
             1790  CGGCCG              
EcoRI         593  GAATTC               G^AATTC
             2823  GAATTC              
FauI         1937  CCCGC                CCCGC(4/6)
             1332  GCGGG               
FbaI          126  TGATCA               T^GATCA
             2546  TGATCA              
HgaI         1606  GACGC                GACGC(5/10)
             3032  GACGC               
HhaI          217  GCGC                 GCG^C
             1526  GCGC                
HinP1I        217  GCGC                 G^CGC
             1526  GCGC                
MfeI          741  CAATTG               C^AATTG
             2270  CAATTG              
MunI          741  CAATTG               C^AATTG
             2270  CAATTG              
PstI           35  CTGCAG               CTGCA^G
              854  CTGCAG              
XmnI         1687  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2625  GAACGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AspI        AvaII       BbsI        
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
Hin1I       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PflFI       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     XhoII       
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