Restriction map for pF1KB3513
 (Linear) (Six-base) MAPSORT of: KIBB3513.seq  Check: 2708  from: 1  to: 3069



KIBB3513.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2758    3069 
   Size:       2758     311
 
AccI GT'mk_AC

Cuts at:      0     776    3069 
   Size:        776    2293
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124    1226    1718    3069 
   Size:        124    1102     492    1351

  Fragments arranged by size: 

                1351    1102     492     124
 
AflII C'TTAA_G

Cuts at:      0    1327    3069 
   Size:       1327    1742
 
AflIII A'CryG_T

Cuts at:      0    1169    3069 
   Size:       1169    1900
 
AleI CACnn'nnGTG

Cuts at:      0     889    2000    3069 
   Size:        889    1111    1069

  Fragments arranged by size: 

                1111    1069     889
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     645    3069 
   Size:        645    2424
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     857     889    3069 
   Size:        857      32    2180

  Fragments arranged by size: 

                2180     857      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1587    2586    3069 
   Size:       1587     999     483

  Fragments arranged by size: 

                1587     999     483
 
ApoI r'AATT_y

Cuts at:      0     593    1728    2823    3069 
   Size:        593    1135    1095     246

  Fragments arranged by size: 

                1135    1095     593     246
 
AvaI C'yCGr_G

Cuts at:      0    2353    3069 
   Size:       2353     716
 
AvrII C'CTAG_G

Cuts at:      0    1656    3069 
   Size:       1656    1413
 
BamHI G'GATC_C

Cuts at:      0    1538    3069 
   Size:       1538    1531
 
BanI G'GyrC_C

Cuts at:      0     754    1525    3069 
   Size:        754     771    1544

  Fragments arranged by size: 

                1544     771     754
 
BanII G_rGCy'C

Cuts at:      0    1502    1763    3069 
   Size:       1502     261    1306

  Fragments arranged by size: 

                1502    1306     261
 
BbeI G_GCGC'C

Cuts at:      0    1529    3069 
   Size:       1529    1540
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1931    2702    2823    3069 
   Size:       1931     771     121     246

  Fragments arranged by size: 

                1931     771     246     121
 
BbvCI CC'TCA_GC

Cuts at:      0     497    2084    3069 
   Size:        497    1587     985

  Fragments arranged by size: 

                1587     985     497
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2951    3069 
   Size:       2951     118
 
BclI T'GATC_A

Cuts at:      0     126    2546    3069 
   Size:        126    2420     523

  Fragments arranged by size: 

                2420     523     126
 
BglI GCCn_nnn'nGGC

Cuts at:      0    3041    3069 
   Size:       3041      28
 
BglII A'GATC_T

Cuts at:      0    2855    3069 
   Size:       2855     214
 
Bme1580I G_kGCm'C

Cuts at:      0    2440    3069 
   Size:       2440     629
 
BmgBI CAC'GTC

Cuts at:      0      19    1109    3069 
   Size:         19    1090    1960

  Fragments arranged by size: 

                1960    1090      19
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     791    1945    2413    2676    3069 
   Size:        791    1154     468     263     393

  Fragments arranged by size: 

                1154     791     468     393     263
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     668     730    2150    2300    3069 
   Size:        668      62    1420     150     769

  Fragments arranged by size: 

                1420     769     668     150      62
 
Bpu10I CC'TnA_GC

Cuts at:      0     497    2084    3069 
   Size:        497    1587     985

  Fragments arranged by size: 

                1587     985     497
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     195     958     973    3069 
   Size:        195     763      15    2096

  Fragments arranged by size: 

                2096     763     195      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     581     686    1087    1847    1909    3069 
   Size:        581     105     401     760      62    1160

  Fragments arranged by size: 

                1160     760     581     401     105      62
 
BsaAI yAC'GTr

Cuts at:      0     763    3069 
   Size:        763    2306
 
BsaBI GATnn'nnATC

Cuts at:      0     189    3069 
   Size:        189    2880
 
BsaHI Gr'CG_yC

Cuts at:      0    1526    1607    3033    3069 
   Size:       1526      81    1426      36

  Fragments arranged by size: 

                1526    1426      81      36
 
BsaWI w'CCGG_w

Cuts at:      0    1151    3069 
   Size:       1151    1918
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     231     261     666     696    1637    1667    2005    2035 
   Size:        231      30     405      30     941      30     338      30

Cuts at:   2035    3069 
   Size:       1034

  Fragments arranged by size: 

                1034     941     405     338     231      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     599     636    1555    1609    3069 
   Size:        599      37     919      54    1460

  Fragments arranged by size: 

                1460     919     599      54      37
 
BseYI C'CCAG_C

Cuts at:      0    1632    3044    3069 
   Size:       1632    1412      25

  Fragments arranged by size: 

                1632    1412      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     221    2806    3069 
   Size:        221    2585     263

  Fragments arranged by size: 

                2585     263     221
 
BsiEI CG_ry'CG

Cuts at:      0    1793    3069 
   Size:       1793    1276
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     706    3069 
   Size:        706    2363
 
Bsp1286I G_dGCh'C

Cuts at:      0    1502    1763    2440    3069 
   Size:       1502     261     677     629

  Fragments arranged by size: 

                1502     677     629     261
 
BspHI T'CATG_A

Cuts at:      0    2492    3069 
   Size:       2492     577
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1264    2758    3069 
   Size:       1264    1494     311

  Fragments arranged by size: 

                1494    1264     311
 
BsrBI CCG'CTC

Cuts at:      0    2541    3069 
   Size:       2541     528
 
BsrDI GCAATG_nn'

Cuts at:      0     722     848    1917    1928    2324    3069 
   Size:        722     126    1069      11     396     745

  Fragments arranged by size: 

                1069     745     722     396     126      11
 
BssSI C'ACGA_G

Cuts at:      0     197    3069 
   Size:        197    2872
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1348    3069 
   Size:       1348    1721
 
BstBI TT'CG_AA

Cuts at:      0     516    3069 
   Size:        516    2553
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1050    1890    2463    3069 
   Size:       1050     840     573     606

  Fragments arranged by size: 

                1050     840     606     573
 
BstYI r'GATC_y

Cuts at:      0     209     564    1538    2855    3069 
   Size:        209     355     974    1317     214

  Fragments arranged by size: 

                1317     974     355     214     209
 
BtgI C'CryG_G

Cuts at:      0    1569    1783    1805    1919    3069 
   Size:       1569     214      22     114    1150

  Fragments arranged by size: 

                1569    1150     214     114      22
 
BtsI GCAGTG_nn'

Cuts at:      0    1298    2007    2719    2794    3069 
   Size:       1298     709     712      75     275

  Fragments arranged by size: 

                1298     712     709     275      75
 
ClaI AT'CG_AT

Cuts at:      0    1739    3069 
   Size:       1739    1330
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1101    3069 
   Size:       1101    1968
 
EaeI y'GGCC_r

Cuts at:      0    1010    1790    3069 
   Size:       1010     780    1279

  Fragments arranged by size: 

                1279    1010     780
 
EagI C'GGCC_G

Cuts at:      0    1790    3069 
   Size:       1790    1279
 
EarI CTCTTCn'nnn_

Cuts at:      0     359    2491    2873    2990    3069 
   Size:        359    2132     382     117      79

  Fragments arranged by size: 

                2132     382     359     117      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     668     730    1226    1718    2150    2300    3069 
   Size:        124     544      62     496     492     432     150     769

  Fragments arranged by size: 

                 769     544     496     492     432     150     124      62
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1591    3069 
   Size:       1591    1478
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1638    2075    2583    3069 
   Size:       1638     437     508     486

  Fragments arranged by size: 

                1638     508     486     437
 
EcoRI G'AATT_C

Cuts at:      0     593    2823    3069 
   Size:        593    2230     246

  Fragments arranged by size: 

                2230     593     246
 
HaeII r_GCGC'y

Cuts at:      0    1529    3069 
   Size:       1529    1540
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     631     663     666     698    1197    1229    1637    1669 
   Size:        631      32       3      32     499      32     408      32

Cuts at:   1669    1764    1796    2005    2037    2067    2099    3069 
   Size:         95      32     209      32      30      32     970

  Fragments arranged by size: 

                 970     631     499     408     209      95      32      32
                  32      32      32      32      32      30       3
 
KasI G'GCGC_C

Cuts at:      0    1525    3069 
   Size:       1525    1544
 
MfeI C'AATT_G

Cuts at:      0     741    2270    3069 
   Size:        741    1529     799

  Fragments arranged by size: 

                1529     799     741
 
MscI TGG'CCA

Cuts at:      0    1012    3069 
   Size:       1012    2057
 
MslI CAynn'nnrTG

Cuts at:      0     796     889    1768    2000    2101    2443    3069 
   Size:        796      93     879     232     101     342     626

  Fragments arranged by size: 

                 879     796     626     342     232     101      93
 
MspA1I CmG'CkG

Cuts at:      0      40     205    2031    2678    3069 
   Size:         40     165    1826     647     391

  Fragments arranged by size: 

                1826     647     391     165      40
 
NarI GG'CG_CC

Cuts at:      0    1526    3069 
   Size:       1526    1543
 
NspI r_CATG'y

Cuts at:      0    1173    3069 
   Size:       1173    1896
 
PasI CC'CwG_GG

Cuts at:      0    1970    2586    3069 
   Size:       1970     616     483

  Fragments arranged by size: 

                1970     616     483
 
PciI A'CATG_T

Cuts at:      0    1169    3069 
   Size:       1169    1900
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2586    3069 
   Size:       2586     483
 
PmlI CAC'GTG

Cuts at:      0     763    3069 
   Size:        763    2306
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1128    1160    3069 
   Size:       1128      32    1909

  Fragments arranged by size: 

                1909    1128      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2075    3069 
   Size:       2075     994
 
PstI C_TGCA'G

Cuts at:      0      39     858    3069 
   Size:         39     819    2211

  Fragments arranged by size: 

                2211     819      39
 
PvuII CAG'CTG

Cuts at:      0      40     205    2031    3069 
   Size:         40     165    1826    1038

  Fragments arranged by size: 

                1826    1038     165      40
 
SexAI A'CCwGG_T

Cuts at:      0    2956    3069 
   Size:       2956     113
 
SfcI C'TryA_G

Cuts at:      0      35     359     854    3069 
   Size:         35     324     495    2215

  Fragments arranged by size: 

                2215     495     324      35
 
SfoI GGC'GCC

Cuts at:      0    1527    3069 
   Size:       1527    1542
 
SmlI C'TyrA_G

Cuts at:      0     174     937     952    1327    3069 
   Size:        174     763      15     375    1742

  Fragments arranged by size: 

                1742     763     375     174      15
 
StuI AGG'CCT

Cuts at:      0    1984    3069 
   Size:       1984    1085
 
StyI C'CwwG_G

Cuts at:      0    1097    1656    1979    3069 
   Size:       1097     559     323    1090

  Fragments arranged by size: 

                1097    1090     559     323
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     385     605     895    2817    2911    3036    3069 
   Size:        385     220     290    1922      94     125      33

  Fragments arranged by size: 

                1922     385     290     220     125      94      33
 
TatI w'GTAC_w

Cuts at:      0     941    1992    3069 
   Size:        941    1051    1077

  Fragments arranged by size: 

                1077    1051     941
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     231     263    3069 
   Size:        231      32    2806

  Fragments arranged by size: 

                2806     231      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     678    2800    3007    3069 
   Size:        678    2122     207      62

  Fragments arranged by size: 

                2122     678     207      62
 
XmnI GAAnn'nnTTC

Cuts at:      0    1691    2629    3069 
   Size:       1691     938     440

  Fragments arranged by size: 

                1691     938     440
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AleI     AlfI     AloI
    AlwNI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI     BglI    BglII Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI     BtsI     ClaI     DrdI     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I     KasI     MfeI
     MscI     MslI   MspA1I     NarI     NspI     PasI     PciI    PflMI
     PmlI     PpiI    PpuMI     PstI    PvuII    SexAI     SfcI     SfoI
     SmlI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     ApaI    ApaLI
     AscI     AseI    AsiSI     BaeI     BcgI    BfrBI     BlpI     BmtI
     BplI  BsiHKAI    BsiWI     BsmI    BspEI    BsrFI    BsrGI   BssHII
   BstEII  BstZ17I   Bsu36I    BtgZI    CspCI     DraI   DraIII     EciI
  EcoICRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PfoI     PmeI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     XbaI
     XcmI     XhoI     XmaI     ZraI
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