Restriction map for pF1KB3512
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BcnI        BfaI        BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRV       EcoT22I     
FseI        HgaI        Hin1I       HindIII     HpaI        
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MluI        MluNI       MroI        
MscI        MslI        MspA1I      MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspI        SwaI        
TaqI        Tth111I     TthHB8I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcsI          176  GAATTC               [GA]^AATT[CT]
AflII         415  CTTAAG               C^TTAAG
AlwI         1156  GGATC                GGATC(4/5)
ApoI          176  GAATTC               [GA]^AATT[CT]
AseI          952  ATTAAT               AT^TAAT
AsnI          952  ATTAAT               AT^TAAT
AvaII         354  GGACC                G^G[AT]CC
AviII         717  TGCGCA               TGC^GCA
BanII          40  GAGCCC               G[GA]GC[CT]^C
BfrI          415  CTTAAG               C^TTAAG
BglII         411  AGATCT               A^GATCT
BmyI           40  GAGCCC               G[AGT]GC[ACT]^C
BsaBI        1157  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          328  GGTCTC               GGTCTC(1/5)
BsmFI          70  GGGAC                GGGAC(10/14)
Bsp1286I       40  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      592  TGTACA               T^GTACA
BspMI         399  GCAGGT               ACCTGC(4/8)
BsrGI         592  TGTACA               T^GTACA
BstAPI       1162  GCATCCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII         18  GGTAACC              G^GT[ACGT]ACC
BstPI          18  GGTAACC              G^GT[ACGT]ACC
BstYI         411  AGATCT               [GA]^GATC[CT]
EcoO65I        18  GGTAACC              G^GT[ACGT]ACC
EcoRI         176  GAATTC               G^AATTC
FauI          621  GCGGG                CCCGC(4/6)
FspI          717  TGCGCA               TGC^GCA
HaeII         587  GGCGCT               [GA]GCGC^[CT]
HapII         162  CCGG                 C^CGG
HincII        223  GTCAAC               GT[CT]^[GA]AC
HindII        223  GTCAAC               GT[CT]^[GA]AC
HpaII         162  CCGG                 C^CGG
MamI         1157  GATCAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1145  CAATTG               C^AATTG
MflI          411  AGATCT               [GA]^GATC[CT]
MspI          162  CCGG                 C^CGG
MunI         1145  CAATTG               C^AATTG
NcoI          334  CCATGG               C^CATGG
NdeI          496  CATATG               CA^TATG
NlaIV        1083  GGAGCC               GG[ACGT]^[ACGT]CC
PflMI         190  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         353  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         952  ATTAAT               AT^TAAT
SphI          183  GCATGC               GCATG^C
SspBI         592  TGTACA               T^GTACA
StuI         1094  AGGCCT               AGG^CCT
Van91I        190  CCAGATGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          190  CCAGATGCTGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         411  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          974  CCGC                 CCGC(-3/-1)
              621  GCGG                
AluI          617  AGCT                 AG^CT
              888  AGCT                
AlwNI         191  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              352  CAGGACCTG           
Asp700I         7  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              172  GAAGGAATTC          
BclI          708  TGATCA               T^GATCA
              743  TGATCA              
BglI          961  GCCATGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              973  GCCGCCCAGGC         
BsgI          397  GTGCAG               GTGCAG(16/14)
             1139  GTGCAG              
BsiYI         190  CCAGATGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1166  CCAATGCAGGG         
BslI          190  CCAGATGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
             1166  CCAATGCAGGG         
CfoI          588  GCGC                 GCG^C
              718  GCGC                
DraII         353  AGGACCT              [GA]G^G[ACGT]CC[CT]
              679  AGGGCCT             
DsaI           86  CCACGG               C^C[GA][CT]GG
              334  CCATGG              
EcoO109I      353  AGGACCT              [GA]G^G[ACGT]CC[CT]
              679  AGGGCCT             
EcoT14I       334  CCATGG               C^C[AT][AT]GG
             1065  CCAAGG              
FbaI          708  TGATCA               T^GATCA
              743  TGATCA              
HhaI          588  GCGC                 GCG^C
              718  GCGC                
HinP1I        588  GCGC                 G^CGC
              718  GCGC                
SfcI          126  CTACAG               C^T[GA][CT]AG
              988  CTACAG              
SmlI          415  CTTAAG               C^T[CT][GA]AG
             1206  CTCAAG              
StyI          334  CCATGG               C^C[AT][AT]GG
             1065  CCAAGG              
Tsp45I        486  GTCAC                ^GT[GC]AC
              518  GTGAC               
XmnI            7  GAATATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              172  GAAGGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AfaI        BbsI        BbvI        BpuAI       BsaJI       
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MvaI        MwoI        NdeII       NlaIII      NspI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
TfiI        Tru9I       TseI        Tsp509I     TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com