Restriction map for pF1KB3505
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BspDI       BspEI       
BspHI       BspMI       BsrFI       BssSI       Bst1107I    
BstEII      BstPI       BstZ17I     Bsu36I      Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     FauI        FbaI        
FseI        FspI        HaeIII      HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MluI        
MluNI       MroI        MscI        MslI        NaeI        
NarI        NcoI        NgoMIV      NlaIV       NotI        
NruI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SfiI        SfoI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          217  GTCTAC               GT^[AC][GT]AC
AflIII        970  ACATGT               A^C[GA][CT]GT
BanII         840  GAGCCC               G[GA]GC[CT]^C
BbsI          922  GAAGAC               GAAGAC(2/6)
BlpI           12  GCTGAGC              GC^T[ACGT]AGC
BmyI          840  GAGCCC               G[AGT]GC[ACT]^C
Bpu1102I       12  GCTGAGC              GC^T[ACGT]AGC
BpuAI         922  GAAGAC               GAAGAC(2/6)
BsaI         1054  GGTCTC               GGTCTC(1/5)
BseRI         190  GAGGAG               GAGGAG(10/8)
BsiEI          57  CGACCG               CG[GA][CT]^CG
BsiYI          66  CCTCCGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           66  CCTCCGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI        1055  GTCTC                GTCTC(1/5)
BsmI          725  GAATGC               GAATGC(1/-1)
Bsp1286I      840  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      405  TGTACA               T^GTACA
BspLU11I      970  ACATGT               A^CATGT
BsrDI         637  CATTGC               GCAATG(2/0)
BsrGI         405  TGTACA               T^GTACA
BsrI          234  CCAGT                ACTGG(1/-1)
BssHII         91  GCGCGC               G^CGCGC
BstAPI        361  GCAGAACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         643  TTCGAA               TT^CGAA
BstNI         693  CCTGG                CC^[AT]GG
BstXI         168  CCATGAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII          12  GCTGAGC              GC^T[ACGT]AGC
DraI          586  TTTAAA               TTT^AAA
DraIII        677  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI          66  CCTCCGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        693  CCTGG                ^CC[AT]GG
EcoT22I       727  ATGCAT               ATGCA^T
HaeII          16  AGCGCC               [GA]GCGC^[CT]
HapII         297  CCGG                 C^CGG
HpaII         297  CCGG                 C^CGG
MfeI          143  CAATTG               C^AATTG
MspA1I        896  CAGCTG               C[AC]G^C[GT]G
MspI          297  CCGG                 C^CGG
MunI          143  CAATTG               C^AATTG
MvaI          693  CCTGG                CC^[AT]GG
NheI          327  GCTAGC               G^CTAGC
NsiI          727  ATGCAT               ATGCA^T
NspI          970  ACATGT               [GA]CATG^[CT]
NspV          643  TTCGAA               TT^CGAA
PvuII         896  CAGCTG               CAG^CTG
SexAI         692  ACCTGGT              A^CC[AT]GGT
SfuI          643  TTCGAA               TT^CGAA
SmaI          296  CCCGGG               CCC^GGG
SspBI         405  TGTACA               T^GTACA
XmaCI         296  CCCGGG               C^CCGGG
XmaI          296  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII          92  CGCG                 CG^CG
               94  CGCG                
AvaI          296  CCCGGG               C^[CT]CG[GA]G
              428  CCCGAG              
BcnI          296  CCCGG                CC^[GC]GG
              297  CCGGG               
BglII         576  AGATCT               A^GATCT
              613  AGATCT              
BpmI          332  CTGGAG               CTGGAG(16/14)
              371  CTGGAG              
BsaJI          20  CCGAGG               C^C[ACGT][ACGT]GG
              296  CCCGGG              
BsgI            6  CTGCAC               GTGCAG(16/14)
              994  GTGCAG              
BsoBI         296  CCCGGG               C^[CT]CG[GA]G
              428  CCCGAG              
BsrBI          40  GAGCGG               CCGCTC(-3/-3)
              619  GAGCGG              
BstUI          92  CGCG                 CG^CG
               94  CGCG                
EarI          187  GAAGAG               CTCTTC(1/4)
              889  GAAGAG              
HphI          124  GGTGA                GGTGA(8/7)
              286  GGTGA               
Ksp632I       187  GAAGAG               CTCTTC(1/4)
              889  GAAGAG              
MseI          443  TTAA                 T^TAA
              587  TTAA                
MvnI           92  CGCG                 CG^CG
               94  CGCG                
NciI          296  CCCGG                CC^[GC]GG
              297  CCGGG               
NdeI          756  CATATG               CA^TATG
              884  CATATG              
PleI          289  GACTC                GAGTC(4/5)
              507  GACTC               
PstI          359  CTGCAG               CTGCA^G
              452  CTGCAG              
SfaNI         307  GATGC                GCATC(5/9)
             1009  GATGC               
TaqI           56  TCGA                 T^CGA
              644  TCGA                
Tru9I         443  TTAA                 T^TAA
              587  TTAA                
TspRI         212  CAGTG                CA[GC]TG[ACGT][ACGT]^
              677  CACTG               
TthHB8I        56  TCGA                 T^CGA
              644  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BfaI        BssKI       BstF5I      BstYI       Cac8I       
CfoI        DdeI        DpnII       Fnu4HI      FokI        
HhaI        HinP1I      HinfI       ItaI        MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MwoI        NdeII       NlaIII      RsaI        Sau3AI      
ScrFI       SfcI        TfiI        TseI        Tsp45I      
Tsp509I     XhoII       
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