Restriction map for pF1KB3467
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspHI       AspI        AviII       
AvrII       BamHI       BanII       BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfrI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspMI       BsrDI       BsrFI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HapII       HgaI        HindIII     HpaI        HpaII       
KpnI        KspI        MamI        MfeI        MluI        
MroI        MspA1I      MspI        MunI        NaeI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    StuI        SwaI        Tth111I     
Van91I      XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII         974  GACGTC               GACGT^C
AccII        1849  CGCG                 CG^CG
AhdI         1276  GACAAACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1156  CCTAAGG              CC^T[ACGT]AGG
AseI          472  ATTAAT               AT^TAAT
AsnI          472  ATTAAT               AT^TAAT
Asp700I       860  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1276  GACAAACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI           28  CTCGGG               C^[CT]CG[GA]G
BalI         1878  TGGCCA               TGG^CCA
BbeI          782  GGCGCC               GGCGC^C
BciVI        1393  GGATAC               GTATCC(6/5)
BglI         1862  GCCGCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         842  AGATCT               A^GATCT
BmyI          668  GTGCCC               G[AGT]GC[ACT]^C
BseRI         119  CTCCTC               GAGGAG(10/8)
BsgI         1358  GTGCAG               GTGCAG(16/14)
BsiWI        1591  CGTACG               C^GTACG
BsmFI        1596  GGGAC                GGGAC(10/14)
BsoBI          28  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      668  GTGCCC               G[AGT]GC[ACT]^C
Bsp1407I     1575  TGTACA               T^GTACA
BspLU11I     1533  ACATGT               A^CATGT
BsrBI        1461  CCGCTC               CCGCTC(-3/-3)
BsrGI        1575  TGTACA               T^GTACA
BssSI        1178  CTCGTG               CACGAG(-5/-1)
BstUI        1849  CGCG                 CG^CG
Bsu36I       1156  CCTAAGG              CC^T[ACGT]AGG
DraII        1445  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DraIII        422  CACCAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1878  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1276  GACAAACGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1156  CCTAAGG              CC^T[ACGT]AGG
EcoO109I     1445  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HincII       1969  GTTGAC               GT[CT]^[GA]AC
HindII       1969  GTTGAC               GT[CT]^[GA]AC
KasI          782  GGCGCC               G^GCGCC
MluNI        1878  TGGCCA               TGG^CCA
MscI         1878  TGGCCA               TGG^CCA
MslI         1131  CACTATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI         1849  CGCG                 CG^CG
NarI          782  GGCGCC               GG^CGCC
NcoI         1413  CCATGG               C^CATGG
PshBI         472  ATTAAT               AT^TAAT
PstI          692  CTGCAG               CTGCA^G
SfoI          782  GGCGCC               GGC^GCC
SmlI          714  CTTGAG               C^T[CT][GA]AG
SphI          624  GCATGC               GCATG^C
SspBI        1575  TGTACA               T^GTACA
SspI         1651  AATATT               AAT^ATT
XbaI          877  TCTAGA               T^CTAGA
XmnI          860  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcsI          352  AAATTT               [GA]^AATT[CT]
              862  AAATTT              
AcyI          782  GGCGCC               G[GA]^CG[CT]C
              974  GACGTC              
AflIII       1533  ACATGT               A^C[GA][CT]GT
             1599  ACGTGT              
ApoI          352  AAATTT               [GA]^AATT[CT]
              862  AAATTT              
AvaII          36  GGACC                G^G[AT]CC
              736  GGTCC               
BsaHI         782  GGCGCC               G[GA]^CG[CT]C
              974  GACGTC              
BsaI         1345  GAGACC               GGTCTC(1/5)
             1418  GAGACC              
HaeII         107  GGCGCT               [GA]GCGC^[CT]
              782  GGCGCC              
Hin1I         782  GGCGCC               G[GA]^CG[CT]C
              974  GACGTC              
HphI          331  GGTGA                GGTGA(8/7)
             1452  TCACC               
PleI            3  GAGTC                GAGTC(4/5)
              717  GAGTC               
TaqI          930  TCGA                 T^CGA
             1094  TCGA                
Tsp45I        332  GTGAC                ^GT[GC]AC
              383  GTGAC               
TthHB8I       930  TCGA                 T^CGA
             1094  TCGA                
XcmI           39  CCAGCAGTTTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              672  CCAAACAGGGTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BanI        BbvI        BfaI        BpmI        BsaJI       
BsiYI       BslI        BsmAI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DsaI        EarI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       XhoII       
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