Restriction map for pF1KB3467
 (Linear) (Six-base) MAPSORT of: KIBB3467.seq  Check: 4753  from: 1  to: 1986



KIBB3467.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AatII G_ACGT'C

Cuts at:      0     978    1986 
   Size:        978    1008
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     237    1821    1986 
   Size:         53     184    1584     165

  Fragments arranged by size: 

                1584     184     165      53
 
AflIII A'CryG_T

Cuts at:      0    1533    1599    1986 
   Size:       1533      66     387

  Fragments arranged by size: 

                1533     387      66
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1281    1986 
   Size:       1281     705
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     641    1986 
   Size:        641    1345
 
AlwNI CAG_nnn'CTG

Cuts at:      0     377     682    1173    1986 
   Size:        377     305     491     813

  Fragments arranged by size: 

                 813     491     377     305
 
ApoI r'AATT_y

Cuts at:      0     352     862    1986 
   Size:        352     510    1124

  Fragments arranged by size: 

                1124     510     352
 
AseI AT'TA_AT

Cuts at:      0     473    1986 
   Size:        473    1513
 
AvaI C'yCGr_G

Cuts at:      0      28    1986 
   Size:         28    1958
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1386    1419    1986 
   Size:       1386      33     567

  Fragments arranged by size: 

                1386     567      33
 
BanI G'GyrC_C

Cuts at:      0     782     907    1792    1819    1986 
   Size:        782     125     885      27     167

  Fragments arranged by size: 

                 885     782     167     125      27
 
BbeI G_GCGC'C

Cuts at:      0     786    1986 
   Size:        786    1200
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1387    1986 
   Size:       1387     599
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1868    1986 
   Size:       1868     118
 
BglII A'GATC_T

Cuts at:      0     842    1986 
   Size:        842    1144
 
Bme1580I G_kGCm'C

Cuts at:      0     672    1986 
   Size:        672    1314
 
BmgBI CAC'GTC

Cuts at:      0    1600    1986 
   Size:       1600     386
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     476    1986 
   Size:        476    1510
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     476     596     953    1390    1986 
   Size:        476     120     357     437     596

  Fragments arranged by size: 

                 596     476     437     357     120
 
Bpu10I CC'TnA_GC

Cuts at:      0    1464    1986 
   Size:       1464     522
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     735    1986 
   Size:        735    1251
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1339    1412    1986 
   Size:       1339      73     574

  Fragments arranged by size: 

                1339     574      73
 
BsaHI Gr'CG_yC

Cuts at:      0     783     975    1986 
   Size:        783     192    1011

  Fragments arranged by size: 

                1011     783     192
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     787     817    1095    1125    1986 
   Size:        787      30     278      30     861

  Fragments arranged by size: 

                 861     787     278      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     110    1986 
   Size:        110    1876
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1379    1986 
   Size:       1379     607
 
BsiWI C'GTAC_G

Cuts at:      0    1591    1986 
   Size:       1591     395
 
Bsp1286I G_dGCh'C

Cuts at:      0     672    1986 
   Size:        672    1314
 
BsrBI CCG'CTC

Cuts at:      0    1463    1986 
   Size:       1463     523
 
BsrGI T'GTAC_A

Cuts at:      0    1575    1986 
   Size:       1575     411
 
BssSI C'ACGA_G

Cuts at:      0    1178    1986 
   Size:       1178     808
 
BstYI r'GATC_y

Cuts at:      0     217     842    1671    1986 
   Size:        217     625     829     315

  Fragments arranged by size: 

                 829     625     315     217
 
Bsu36I CC'TnA_GG

Cuts at:      0    1157    1986 
   Size:       1157     829
 
BtgI C'CryG_G

Cuts at:      0     327    1413    1881    1986 
   Size:        327    1086     468     105

  Fragments arranged by size: 

                1086     468     327     105
 
BtsI GCAGTG_nn'

Cuts at:      0      20    1872    1986 
   Size:         20    1852     114

  Fragments arranged by size: 

                1852     114      20
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1466    1501    1986 
   Size:       1466      35     485

  Fragments arranged by size: 

                1466     485      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     427    1986 
   Size:        427    1559
 
EaeI y'GGCC_r

Cuts at:      0    1878    1986 
   Size:       1878     108
 
EarI CTCTTCn'nnn_

Cuts at:      0      72    1212    1436    1986 
   Size:         72    1140     224     550

  Fragments arranged by size: 

                1140     550     224      72
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1114    1886    1986 
   Size:       1114     772     100

  Fragments arranged by size: 

                1114     772     100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     237     476     596     953    1390    1821    1986 
   Size:         53     184     239     120     357     437     431     165

  Fragments arranged by size: 

                 437     431     357     239     184     165     120      53
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1446    1986 
   Size:       1446     540
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     264    1465    1986 
   Size:        264    1201     521

  Fragments arranged by size: 

                1201     521     264
 
HaeII r_GCGC'y

Cuts at:      0     111     786    1986 
   Size:        111     675    1200

  Fragments arranged by size: 

                1200     675     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81     391     423     554     586    1161    1193 
   Size:         49      32     310      32     131      32     575      32

Cuts at:   1193    1267    1299    1986 
   Size:         74      32     687

  Fragments arranged by size: 

                 687     575     310     131      74      49      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1971    1986 
   Size:       1971      15
 
KasI G'GCGC_C

Cuts at:      0     782    1986 
   Size:        782    1204
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1037    1649    1986 
   Size:       1037     612     337

  Fragments arranged by size: 

                1037     612     337
 
MscI TGG'CCA

Cuts at:      0    1880    1986 
   Size:       1880     106
 
MslI CAynn'nnrTG

Cuts at:      0    1135    1986 
   Size:       1135     851
 
NarI GG'CG_CC

Cuts at:      0     783    1986 
   Size:        783    1203
 
NcoI C'CATG_G

Cuts at:      0    1413    1986 
   Size:       1413     573
 
NspI r_CATG'y

Cuts at:      0     628    1403    1537    1986 
   Size:        628     775     134     449

  Fragments arranged by size: 

                 775     628     449     134
 
PasI CC'CwG_GG

Cuts at:      0    1962    1986 
   Size:       1962      24
 
PciI A'CATG_T

Cuts at:      0    1533    1986 
   Size:       1533     453
 
PfoI T'CCnGG_A

Cuts at:      0     969    1986 
   Size:        969    1017
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     431     463    1986 
   Size:        431      32    1523

  Fragments arranged by size: 

                1523     431      32
 
PsiI TTA'TAA

Cuts at:      0    1955    1986 
   Size:       1955      31
 
PstI C_TGCA'G

Cuts at:      0     696    1986 
   Size:        696    1290
 
SfcI C'TryA_G

Cuts at:      0     692    1211    1490    1986 
   Size:        692     519     279     496

  Fragments arranged by size: 

                 692     519     496     279
 
SfoI GGC'GCC

Cuts at:      0     784    1986 
   Size:        784    1202
 
SmlI C'TyrA_G

Cuts at:      0     714    1986 
   Size:        714    1272
 
SphI G_CATG'C

Cuts at:      0     628    1986 
   Size:        628    1358
 
SspI AAT'ATT

Cuts at:      0    1653    1986 
   Size:       1653     333
 
StyI C'CwwG_G

Cuts at:      0     806    1413    1611    1986 
   Size:        806     607     198     375

  Fragments arranged by size: 

                 806     607     375     198
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1251    1986 
   Size:       1251     735
 
TatI w'GTAC_w

Cuts at:      0      91     837    1575    1986 
   Size:         91     746     738     411

  Fragments arranged by size: 

                 746     738     411      91
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     965     997    1986 
   Size:        965      32     989

  Fragments arranged by size: 

                 989     965      32
 
XbaI T'CTAG_A

Cuts at:      0     877    1986 
   Size:        877    1109
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      46     679    1986 
   Size:         46     633    1307

  Fragments arranged by size: 

                1307     633      46
 
XmnI GAAnn'nnTTC

Cuts at:      0     864    1986 
   Size:        864    1122
 
ZraI GAC'GTC

Cuts at:      0     976    1986 
   Size:        976    1010
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AhdI     AlfI    AlwNI     ApoI     AseI
     AvaI     BaeI     BanI     BbeI    BciVI     BglI    BglII Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI
    BseRI     BsgI    BsiWI Bsp1286I    BsrBI    BsrGI    BssSI    BstYI
   Bsu36I     BtgI     BtsI    CspCI   DraIII     EaeI     EarI     EciI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII     KasI     MmeI
     MscI     MslI     NarI     NcoI     NspI     PasI     PciI     PfoI
     PpiI     PsiI     PstI     SfcI     SfoI     SmlI     SphI     SspI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI    BanII
     BbsI    BbvCI     BcgI     BclI    BfrBI     BlpI     BmtI     BplI
    BsaAI    BsaBI    BsaWI    BseYI    BsiEI  BsiHKAI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     ClaI     DraI     DrdI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     HpaI     KpnI     MfeI     MluI   MspA1I     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI    PflMI     PmeI     PmlI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI
     SwaI  Tth111I     XhoI     XmaI
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