Restriction map for pF1KB3458
##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BfrI        BlnI        BsaBI       BsiWI       BsmBI       
BspHI       BspLU11I    BsrBI       BssHII      BstBI       
CpoI        DraI        DraIII      Eam1105I    Eco47III    
EcoT22I     FseI        FspI        HpaI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspI        
SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII        3244  GACGTC               GACGT^C
AccI         1071  GTATAC               GT^[AC][GT]AC
AccIII        389  TCCGGA               T^CCGGA
AflIII       2537  ACACGT               A^C[GA][CT]GT
ApaI         3160  GGGCCC               GGGCC^C
BamHI        1373  GGATCC               G^GATCC
BbrPI        2538  CACGTG               CAC^GTG
BbvCI        1396  CCTCAGC              CCTCAGC(-5/-2)
BcgI          876  GCAACAAATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         231  GGATAC               GTATCC(6/5)
BglI          139  GCCCCTCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1317  AGATCT               A^GATCT
BsaI         1301  GAGACC               GGTCTC(1/5)
BseAI         389  TCCGGA               T^CCGGA
BsiEI        1720  CGGCCG               CG[GA][CT]^CG
BsmI          111  GAATGC               GAATGC(1/-1)
BspDI        2026  ATCGAT               AT^CGAT
BspEI         389  TCCGGA               T^CCGGA
BssSI        1858  CACGAG               CACGAG(-5/-1)
Bst1107I     1071  GTATAC               GTA^TAC
BstZ17I      1071  GTATAC               GTA^TAC
ClaI         2026  ATCGAT               AT^CGAT
EagI         1720  CGGCCG               C^GGCCG
EclXI        1720  CGGCCG               C^GGCCG
Eco52I       1720  CGGCCG               C^GGCCG
EcoRI         467  GAATTC               G^AATTC
EcoRV        2137  GATATC               GAT^ATC
HincII        475  GTCAAC               GT[CT]^[GA]AC
HindII        475  GTCAAC               GT[CT]^[GA]AC
HindIII       400  AAGCTT               A^AGCTT
MroI          389  TCCGGA               T^CCGGA
PaeR7I        237  CTCGAG               C^TCGAG
PmaCI        2538  CACGTG               CAC^GTG
PmlI         2538  CACGTG               CAC^GTG
PshAI        1323  GACCCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI         2757  CCTGCAGG             CCTGCA^GG
SexAI        2873  ACCAGGT              A^CC[AT]GGT
SmaI         1325  CCCGGG               CCC^GGG
Sse8387I     2757  CCTGCAGG             CCTGCA^GG
StuI          146  AGGCCT               AGG^CCT
XhoI          237  CTCGAG               C^TCGAG
XmaCI        1325  CCCGGG               C^CCGGG
XmaI         1325  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccII        1820  CGCG                 CG^CG
             1822  CGCG                
AocI          142  CCTCAGG              CC^T[ACGT]AGG
             2777  CCTCAGG             
AspI         3241  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3244  GACGTCGTC           
BbsI         1221  GAAGAC               GAAGAC(2/6)
             3031  GTCTTC              
BclI         3206  TGATCA               T^GATCA
             3312  TGATCA              
BpmI         2215  CTCCAG               CTGGAG(16/14)
             2060  CTGGAG              
BpuAI        1221  GAAGAC               GAAGAC(2/6)
             3031  GTCTTC              
BsaAI         290  TACGTG               [CT]AC^GT[GA]
             2538  CACGTG              
BsaWI         389  TCCGGA               [AT]^CCGG[AT]
             2573  ACCGGA              
Bsp1407I       17  TGTACA               T^GTACA
             2484  TGTACA              
BsrDI        2261  CATTGC               GCAATG(2/0)
             1925  GCAATG              
BsrGI          17  TGTACA               T^GTACA
             2484  TGTACA              
BstUI        1820  CGCG                 CG^CG
             1822  CGCG                
Bsu36I        142  CCTCAGG              CC^T[ACGT]AGG
             2777  CCTCAGG             
DrdI         3238  GACGACGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3241  GACGACGTCGTC        
Eco81I        142  CCTCAGG              CC^T[ACGT]AGG
             2777  CCTCAGG             
FbaI         3206  TGATCA               T^GATCA
             3312  TGATCA              
MseI         2371  TTAA                 T^TAA
             2444  TTAA                
MvnI         1820  CGCG                 CG^CG
             1822  CGCG                
PflFI        3241  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3244  GACGTCGTC           
SapI          658  GCTCTTC              GCTCTTC(1/4)
             1917  GCTCTTC             
ScaI         1127  AGTACT               AGT^ACT
             2528  AGTACT              
SspBI          17  TGTACA               T^GTACA
             2484  TGTACA              
Tru9I        2371  TTAA                 T^TAA
             2444  TTAA                
Tth111I      3241  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3244  GACGTCGTC           
XcmI           30  CCATGTGGCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3215  CCATCAAGGATGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvI        BcnI        BfaI        BlpI        
BmyI        Bpu1102I    BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmlI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI        
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